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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
spore print to syringe
    #7954823 - 01/30/08 09:02 AM (16 years, 2 months ago)

old method poke nail size hole in jar lid cover with tyvek. fill half pint jar with 3/4 water. sterilize 30 min... make shift glove box inverted rubber maid lots of lysol....wait till jar cools. clean syringe with sterilized water inside...flame sterilize the needle shoot a little water out to cool then scrape spores into jar. cover.

then 10 clean syringes with water. flame sterilize.shoot water into jar then suck out

anybody got anything new? i do understand that my writing sucks:evil:

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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: spore print to syringe [Re: Tomandjerry58]
    #7954833 - 01/30/08 09:07 AM (16 years, 2 months ago)

that will work.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Offlinefluffyshapes
lowly lotus
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Registered: 04/02/07
Posts: 257
Loc: Georgia
Last seen: 8 months, 19 days
Re: spore print to syringe [Re: tahoe]
    #7954971 - 01/30/08 10:13 AM (16 years, 2 months ago)

all of this is done in a lysol'd environment.

what i do now is take a folded up print, wipe outside with alcohol, pick it up with flame and alcohol sterilized scissors, place in new ziploc. 

Usually i only make 6 or 7 3cc syringes at a time,
so i cut a strip of the still-folded print off of the whole.

I take out the remaining part of the print, fold it up and put it back, then seal the ziploc.


Then the tricky part is unfolding the strip of print whilst it's still in the ziploc.

then fill the syringes with sterile water.  Usually I just poke them through the ziploc, if they're small gauge needles they work fine. try to squirt the print directly.

Move print and water to the bottom corner, and scrape spores off from the outside.


move the scraped clean foil up the bag, out of the way.

now just stab your syringes through the ziploc, pull back the plunger, and you're set. :smile:


GOOD:
no glove box needed (mine is too small to do this in)

BAD:
depending on how your method of storage, the outside of your spore print might not be something you want mingling in your spore suspension.

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OfflinebrainsOplenty
myconut
Male


Registered: 06/27/06
Posts: 750
Loc: on your sister
Last seen: 1 year, 4 months
Re: spore print to syringe [Re: fluffyshapes]
    #7954991 - 01/30/08 10:22 AM (16 years, 2 months ago)

don't use lysol as it contains petroleum distilliates. just wash glove box or still air box thoroughly with 10% bleach solution.


--------------------
FOR GOD'S SAKE MAN! TELL ME ABOUT THE FUCKING GOLF SHOES!!!


LIVIN THE LIFE!!!


"WE KNOCK NIGGAS OUT AND MAKE EM BOUNCE LIKE RICKY HATTON"- IMMORTAL TECHNIQUE

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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: spore print to syringe [Re: brainsOplenty]
    #7955010 - 01/30/08 10:32 AM (16 years, 2 months ago)

im cool with the lysol.. been using it for awhile and always worked out fine. and im also afraid of airborne contaminates.

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