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Offlinesmokeydablunt
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Registered: 07/29/07
Posts: 67
Last seen: 15 years, 6 months
Contamination?
    #7810672 - 12/29/07 02:22 PM (16 years, 3 months ago)

Hi, i just recently inoculated 6 jars. This was my first go at it. I boguth them pre sterilized. Which probly was my problem. But i didnt have a pressure cooker and stilll dont and im going to buy one soon. Im just lookin around for a deal. But anyways. The jars have ;like a greenish bluish spots on it. I could see them before i could see any mycelium. Heres some pics.


Yea All the jars have this. If they are contaminated. How should i dispose of this. I heard just dump outside in a bag. But how would i clean the jars? Thanks

Edited by smokeydablunt (12/29/07 02:25 PM)

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Offlineguyoncouch
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Registered: 12/11/07
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Re: Contamination? [Re: smokeydablunt]
    #7810678 - 12/29/07 02:26 PM (16 years, 3 months ago)

as for cleanin the jars, bleach would suffice.

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Offlinebeeplus
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Registered: 11/26/07
Posts: 56
Last seen: 13 years, 1 month
Re: Contamination? [Re: smokeydablunt]
    #7810685 - 12/29/07 02:28 PM (16 years, 3 months ago)

definitely trichoderma. there's really nothing you can do at this point. I heard salt works, but only on the spot with the infection. at that point its probably pretty deep in the substrate so i think you're beat.

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Offlinesmokeydablunt
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Re: Contamination? [Re: guyoncouch]
    #7810686 - 12/29/07 02:29 PM (16 years, 3 months ago)

Alright. I just need to get myself a pressure cooker. and im ready to go.

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Offlinebeeplus
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Re: Contamination? [Re: smokeydablunt]
    #7810693 - 12/29/07 02:32 PM (16 years, 3 months ago)

i like that mindset.

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OfflineSubstance D
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Re: Contamination? [Re: beeplus]
    #7810861 - 12/29/07 03:41 PM (16 years, 3 months ago)

if your gonna save the jars make sure you boil them before you open them to kill whats in there.,but if you dont need them just toss them .once again sorry about the loss heres a pic of my cakes after a week [url=[url=/


--------------------
You can turn your back on a person, but, never turn your back on a drug. Especially when it's waving a razor-sharp hunting knife in your eye.

Edited by Substance D (12/29/07 03:43 PM)

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OfflineNibin
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Re: Contamination? [Re: Substance D]
    #7811236 - 12/29/07 05:56 PM (16 years, 3 months ago)

You don't need a pressure cooker for pf cakes even if it is an added bonus. If the jars were presterilized and from a reputable vendor, the chances are it was you who introduced the contamination.


--------------------
Newcomers guide-----> For all things shroomy

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Offlinesmokeydablunt
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Re: Contamination? [Re: Nibin]
    #7811436 - 12/29/07 07:05 PM (16 years, 3 months ago)

I saw that you can use a big pot which i have but what could i use to elevate the jars so that they would be out of the water? I dont have jar lids. and how long would i steam them for and what temp. HOw long should i steam them to kill the mold and how long should i steam them to sterilize the brown rice flour and verm?

Substance d your jars are looking fucking beautiful. Keep it up :smile:

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Offlineveda_sticks
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Re: Contamination? [Re: smokeydablunt]
    #7811476 - 12/29/07 07:19 PM (16 years, 3 months ago)

You can save the jars to reuse them, u dont have to go as far as sterilising but it would be good to kill most of it just incase, getting a face full of spores it not nice.

You can use some scrunched up tinfoil to keep the jars an inch or so of the bottom, then bring the water level to a few inches above the jar.

Bring to a boil and reduce the heat so that its boiling but not rapildy boiling, make sure the lid is tight on, i sat a heavy bowl onto of the lid to help stop steam escaping.

You need to make sure it doesnt boil dry, as its the steam thats doing the sterilising. This is why you want it to be just at boiling point and not bublling furiously otherwise you will boil it dry.

Once it has reached a boil, set your timmer for 90 minutes.

90 minutes should be adequate if done right. When u make up your substrate jars if u feel safer then you can go to 2 hours (i have never had any problems with 90 minutes).

Mould spores will be killed in the steaming process, pf tek jars dont contain endospores which is why you can steam sterilise. If a jar has contaminated then its likely it was introduced during inoculation.


You did use a glove box yes??


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinesmokeydablunt
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Registered: 07/29/07
Posts: 67
Last seen: 15 years, 6 months
Re: Contamination? [Re: veda_sticks]
    #7811494 - 12/29/07 07:27 PM (16 years, 3 months ago)

Yea a ghetto ass one. But i think i found what i did wrong. I had the box in the closet and i couldnt see in there and i had the jars already in there and shit. Then i moved it into alot bigger room. then did it. ANd i think when i moved it. Shit got in there. so yea. thanks for help.

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Offlineveda_sticks
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Re: Contamination? [Re: smokeydablunt]
    #7811515 - 12/29/07 07:38 PM (16 years, 3 months ago)

Moving the box would have drawn in air threw the arm holes stiring up contams which would have.

The princaple of a still air box is not that its sterile, its that the air inside i still so no contams float about. You clean it it, put your stuff in and give it a quick spray which lysol or bleach and let it settle for 10 minutes, then carrie out your inoculations.

Also u should be flaming your needle and wiping it down with alcohol before inocing each jar, this sterilises the needle, u do it also between each jar to prevent cross contamination.

Its a good idea to flame the needle just outside the glovebox as lysol and bleach are flammible. There have been a few horror stories of glove boxes exploding.

Flame outside and bring the neelde inside and wipe it down with the alcohol then inoculate.

doing it in a small cupboard that has been cleaned is good, but i have had no problem just using my glovebox in my hall.

After you have sterilised your jars and they have cooled overnight in the pot, take the pot to the glovebox then open and transfer the jars into the box.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinesmokeydablunt
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Registered: 07/29/07
Posts: 67
Last seen: 15 years, 6 months
Re: Contamination? [Re: veda_sticks]
    #7811541 - 12/29/07 07:46 PM (16 years, 3 months ago)

Yea i did all those steps but i fucked up when i picked it up. I wanna use this big pot i have but i cant stack jars can i ?

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Invisibletahoe
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Registered: 11/26/03
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Re: Contamination? [Re: veda_sticks]
    #7811543 - 12/29/07 07:47 PM (16 years, 3 months ago)

let me guess, spores101??? they have sent out a bunch of dirty syringes

Your cakes are way too wet also. you should get your money back for them.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Offlinesmokeydablunt
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Posts: 67
Last seen: 15 years, 6 months
Re: Contamination? [Re: tahoe]
    #7811555 - 12/29/07 07:51 PM (16 years, 3 months ago)

Naw i got spores from lilshop.

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Offlineveda_sticks
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Re: Contamination? [Re: smokeydablunt]
    #7811586 - 12/29/07 08:02 PM (16 years, 3 months ago)

ACtually they do look wet now that i look at it again, i have had jars successfully colinise looking like that, but took a while.

My last batch i had colinise in about just 25 days (due to only 2 inoc points) had i used 4 inoc points they would have been done in 14. But i see some good rhyzos so its all good.

Them mix should be light and fluffy and look like it could be dry, light floury colour.

Follow the mix exactly, 2 parts verm 1 part brf mixed well then add the water and mix well. Scoop some of the mix away to revel the bottom while tilting the jar to reveal water, only a little should pool just a little amount.

If u get alot of water then drain it off. Too much moisture can slow growth and cause it to stall and in some cases wet rot.

Its all part of the learning process, took me 2 or 3 attempts to stop geting green mould


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinesmokeydablunt
Stranger
Registered: 07/29/07
Posts: 67
Last seen: 15 years, 6 months
Re: Contamination? [Re: veda_sticks]
    #7811626 - 12/29/07 08:16 PM (16 years, 3 months ago)

Isnt there a calculator on mixing verm and shit? I thoguth someone here made one? but can you stack jars just using a regular pot?

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Invisibletahoe
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Re: Contamination? [Re: smokeydablunt]
    #7811635 - 12/29/07 08:18 PM (16 years, 3 months ago)

yes you can stack jar in a regular pot. Just like a pc


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Offlinesmokeydablunt
Stranger
Registered: 07/29/07
Posts: 67
Last seen: 15 years, 6 months
Re: Contamination? [Re: tahoe]
    #7811645 - 12/29/07 08:23 PM (16 years, 3 months ago)

When your a noob this hobby can be expensive.

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