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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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stinkbuttdog
I scat.
Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
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Your "dirty" spore print on agar...
#7780092 - 12/20/07 12:31 AM (16 years, 3 months ago) |
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does it REALLY matter weather or not your print is dirty if you really know how to work the agar gig? Isolation should lead you to a clear specimen, right? (Provided your environmental conditions are releatively clean?) Just wonderin'.
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stinkbuttdog
I scat.
Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
#7780097 - 12/20/07 12:34 AM (16 years, 3 months ago) |
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I guess what I'm asking is, can't you pretty much take a big shit on your print and bring it back from the brink of un-usability with careful agar work? I'm really trying to think "yes", I just need your reassurance because you matter to me!
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FooMan
Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
#7780111 - 12/20/07 12:41 AM (16 years, 3 months ago) |
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Sure, but it isn't as easy as it may sound.
-------------------- Quick WBS Prep
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stinkbuttdog
I scat.
Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
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Re: Your "dirty" spore print on agar... [Re: FooMan]
#7780150 - 12/20/07 12:59 AM (16 years, 3 months ago) |
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I love you FooManShroom!
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anarchOi
Ellenalien is fat.
Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 13 years, 1 month
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
#7780200 - 12/20/07 01:28 AM (16 years, 3 months ago) |
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just don't get too excited when you see the white growing because if your print is dirty, it'll likely be the trich
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stinkbuttdog
I scat.
Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
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Re: Your "dirty" spore print on agar... [Re: anarchOi]
#7780204 - 12/20/07 01:31 AM (16 years, 3 months ago) |
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Quote:
anarchOi said: just don't get too excited when you see the white growing because if your print is dirty, it'll likely be the trich
Distinguishable under the microscope.
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Nibin
Getting there
Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 10 months
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
#7780381 - 12/20/07 03:41 AM (16 years, 3 months ago) |
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With patient, careful work with agar you can isolate the good from the bad. But it can take it's time depending on how bad it was.
-------------------- Newcomers guide-----> For all things shroomy
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mebesideme
o-bliminal
Registered: 08/19/04
Posts: 177
Last seen: 7 years, 3 months
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Re: Your "dirty" spore print on agar... [Re: Nibin]
#7780389 - 12/20/07 03:50 AM (16 years, 3 months ago) |
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As long as your spores are stored in a viable form where they aren't being attacked/eaten without being able to germinate, I sdee no reason you couldn't recover something from pretty much any condition.
-------------------- Doot de dooh.... yep yep yep. A signature and stuff...
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Crake
Stranger
Registered: 12/04/07
Posts: 194
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Re: Your "dirty" spore print on agar... [Re: Nibin]
#7780392 - 12/20/07 03:56 AM (16 years, 3 months ago) |
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I think Stinkbutt has made his/her point in case. Indeed, one could actually take a shit on the print... lol, and raise a strong specimen (of bacteria, virus or fungus) from this print.
Agar is the light at the end of the tunnel. I strongly encourage all to invest time and/or money into this facet, and adjacent methodology of cultivation as it propagates an ultimately stronger specimen.
-------------------- MANGO Snowman wakes before dawn. He lies unmoving, listening to the tide coming in, wave after wave sloshing over the various barricades, wish-wash, wish-wash, the rhythm of heartbeat. He would so like to believe he is still asleep.
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stinkbuttdog
I scat.
Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
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Re: Your "dirty" spore print on agar... [Re: Crake]
#7780396 - 12/20/07 04:15 AM (16 years, 3 months ago) |
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Much love,...:D
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: Your "dirty" spore print on agar... [Re: stinkbuttdog]
#7780537 - 12/20/07 06:26 AM (16 years, 3 months ago) |
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Agar is a recomended method. Prints are not sterile, while it is possible to make a viable syringe from a print, using agar has the advantage that contamination can be seperated from clean myc via transfering to new plates.
As long as you get spores to germinate and grow enough myc to get a even just a tiny peice of tissue. You can transfer to a new plate, and grow it out. Contam shows up?? Transfer again.
There is also the advantage that while you are transfering you can start sectoring and isolating.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: Your "dirty" spore print on agar... [Re: veda_sticks]
#7780555 - 12/20/07 06:43 AM (16 years, 3 months ago) |
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I would like to add that agar also be good for cloning, i have read a few posts here and there were some people just had a hell of a time will LC cloning.
AGAR!!!
yup.
One day, when i buy my own house, i will have my own we lab.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Brainiac
Rogue Scientist
Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋
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Re: Your "dirty" spore print on agar... [Re: veda_sticks]
#7780701 - 12/20/07 07:58 AM (16 years, 3 months ago) |
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Some people use brown box cardbored to start spores, MR..
-------------------- Fair is Fair
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anarchOi
Ellenalien is fat.
Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 13 years, 1 month
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Re: Your "dirty" spore print on agar... [Re: Brainiac]
#7780903 - 12/20/07 09:26 AM (16 years, 3 months ago) |
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LCs suck ass for cloning if you can't get agar i still highly reccomend the BRF substitute
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 29 days
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Re: Your "dirty" spore print on agar... [Re: anarchOi]
#7781026 - 12/20/07 10:24 AM (16 years, 3 months ago) |
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Agar is the gold standard for isolating because the mycelium and contaminants only grow on the surface where they're visible and available for transfer with a scalpel. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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SuchSmartMonkeys
mycologically driven individual
Registered: 10/26/05
Posts: 1,154
Last seen: 3 years, 20 days
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Re: Your "dirty" spore print on agar... [Re: veda_sticks]
#10195184 - 04/19/09 01:05 PM (14 years, 10 months ago) |
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Quote:
veda_sticks said: I would like to add that agar also be good for cloning, i have read a few posts here and there were some people just had a hell of a time will LC cloning.
AGAR!!!
yup.
One day, when i buy my own house, i will have my own we lab.
oh, you don't need a lab... All you need is a glovebox. I've done some agar work in some shifty spots with very little contam... If there's a will, there's a way in this hobby, hehe
and how would you isolate anything with LC? You just have a bunch of mycelium suspended in liquid, there's no way to tell strains apart from eachother, no way to look for favorable growth, and it seems like it would be tough to make a transfer from a specific colony, even if the colonies were distinguishable... When an LC has contam in it, it's often times hard to tell the difference between the contam and the healthy mycelium of whatever you're going for. Has anyone ever used LC for "isolation"?
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