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Offlinekatfish
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Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 16 years, 1 month
pouring agar for the first time, plz help!
    #7734735 - 12/08/07 09:06 PM (16 years, 3 months ago)

What up guys! I am having a PCin party at my friends house since he is letting me use his stove for my PC. I have some nutrient agar powder that I had mixed with some water and stirred up and sealed the jar, its a half pint jar btw. My friend just went to my other place to pick it up and I am about to put it in some boilling water to get it to the right tempature to start pouring plates.

I have some small petri dishes, i mean they are around half the size of a normal dish. I figure they will colonize quickly, not letting contams get a chance to form.

Is it possible to pour these without a glovebox?

Or will I just contaminate them everytime>

RR sugessted the in substitute for a golvebox you can use a clear trash bag and just put the items way deep in it. Can this workaround be used for somethign like pouring agar plates or slants?

Also can I just pour the agar into half pint jars? I mean I know you can use test tubes and petri dishes, is there any containers that agar doesn't like?


thanks for any help!

kF


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!

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OfflineNibin
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Re: pouring agar for the first time, plz help! [Re: katfish]
    #7734753 - 12/08/07 09:09 PM (16 years, 3 months ago)

Katfish, with all the problems you have had with contams, why don't you go out and buy a rubbermaid to make your glovebox with. They are cheap.


--------------------
Newcomers guide-----> For all things shroomy

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OfflinePr0_X
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
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Re: pouring agar for the first time, plz help! [Re: Nibin]
    #7734966 - 12/08/07 10:15 PM (16 years, 3 months ago)

Clear plastic garbage bag "should" work but there is no gaurntee, build yourself a glove box for agar otherwise it will contam everytime.

You can not expose a source of nutrients to open air or it will contam every single time.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl

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Offlineseg_x
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Registered: 08/31/06
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Re: pouring agar for the first time, plz help! [Re: Pr0_X]
    #7735183 - 12/08/07 11:52 PM (16 years, 3 months ago)

Yea, a rubbermaid glovebox is worth the effort.
If your getting into a more complex method such as cloning
or anything using agar... dont skip steps get your glovebox.

I made the mistake of skipping steps and ended up wasting alot of
money when I could have went about it the right way.

Plus a rubbermaid glovebox makes a good storage box for your
supplies (sprays, extra jars, etc.) when not in use.

Edited by seg_x (12/09/07 01:35 AM)

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Offlinekatfish
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Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 16 years, 1 month
Re: pouring agar for the first time, plz help! [Re: seg_x]
    #7735352 - 12/09/07 01:21 AM (16 years, 3 months ago)

yeah. i am definitly gonna make a glovebox, soon, i had the storage bin and the yellow Mr. Clean gloves, but I cracked the rubbermaid when I was trying to cut the holes. I am gonna get another plastic bin tommorrow and try again. I need a good drill and a hole saw.

kF


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!

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Offlineseg_x
was here,chillin
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Registered: 08/31/06
Posts: 368
Loc: Some place south.
Last seen: 8 years, 7 months
Re: pouring agar for the first time, plz help! [Re: katfish]
    #7735380 - 12/09/07 01:32 AM (16 years, 3 months ago)

You can lay the tub on its side and cut thru the lid,
much softer plastic on the lid. the only thing I disliked about
doing the glovebox on its side, is that it makes the box unlevel.
So I used a book to even it out. idk, just an idea.


--------------------


My Fall Mushrooms

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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: pouring agar for the first time, plz help! [Re: seg_x]
    #7735399 - 12/09/07 01:41 AM (16 years, 3 months ago)



--------------------


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OfflineIolaa
iolaa, not lolaa
Registered: 08/28/07
Posts: 335
Loc: Humco
Last seen: 6 months, 5 days
Re: pouring agar for the first time, plz help! [Re: seg_x]
    #7735414 - 12/09/07 01:48 AM (16 years, 3 months ago)

YES! cut through the lid. that's the way i did mine, and i really love it. the only thing you have to worry about is attaching the gloves. i highly reccomend 4" PVC couplers, and taping gloves around them. there's a tek somewhere on here. also, don't get a box that is too big. otherwise you'll lose hold of something and and it will roll into a corner and you won't be able to reach it.

glove boxes are awkward as HELL to work with, but worth it in the end. good luck!

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Invisibleroquet
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Registered: 05/29/07
Posts: 1,195
Loc: Dubai بجدية عربي...
Re: pouring agar for the first time, plz help! [Re: katfish]
    #7735582 - 12/09/07 06:29 AM (16 years, 3 months ago)

Quote:

katfish said:
yeah. i am definitly gonna make a glovebox, soon, i had the storage bin and the yellow Mr. Clean gloves, but I cracked the rubbermaid when I was trying to cut the holes. I am gonna get another plastic bin tommorrow and try again. I need a good drill and a hole saw.



I cracked my first glove box trying to cut it. Heat a knife in your kitchen hob flame and melt holes one after along the line of your circle. It works!

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OfflineTheFish
Shakedown Street
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Registered: 10/07/03
Posts: 440
Last seen: 8 years, 8 months
Re: pouring agar for the first time, plz help! [Re: Pr0_X]
    #7735981 - 12/09/07 10:21 AM (16 years, 3 months ago)

Quote:

Pr0_X said:You can not expose a source of nutrients to open air or it will contam every single time.




You cannot make this statement with a 100% guarantee.

If you're pc'ing your own agar, you should have no problem pouring plates in open air as long as you're cleaning your work area well. I've poured 50-60 open air plates and had 2 or 3 contaminations.

Allow your agar to cool just before solidification to prevent condensation on the lid of the dish.


--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."

Jerry Garcia

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Offlinekatfish
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Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 16 years, 1 month
Re: pouring agar for the first time, plz help! [Re: TheFish]
    #7736801 - 12/09/07 02:03 PM (16 years, 3 months ago)

well. i will go see my plates today and give a report, I have them cooling at my brothers hbouse.

kF


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!

Extras: Filter Print Post Top
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