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Offlineresptodd
I reject yourreality andsubstitute myown
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Registered: 10/16/07
Posts: 674
Loc: Michigan
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Germ. time
    #7574355 - 10/29/07 08:25 PM (16 years, 4 months ago)

So here I am, 1 week into inoc. with nothing to show. What is the average time to see myc growth? I am afraid I screwed up. I don't have alot of money to be messing around with, but I have very good sterile skills. I put forth plenty of energy inoculating both PF tek and grain jars, planning to transfer to Hpoo/straw/verm. Hate to name the distributer of the syringes, but visually the spore count was less than what I have seen from friends syringes. Could I have bought poor quality syringes?


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InvisibleThis guy
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Registered: 07/28/07
Posts: 183
Re: Germ. time [Re: resptodd]
    #7574377 - 10/29/07 08:33 PM (16 years, 4 months ago)

from a multispore inoculation i start to see growth anywhere between 4-7 days..

you dont have to see spores for them to be there...

you dont have to mention the vendors name.. but was it from one of the sponsors on the board?

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Offlinebudzeno
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Registered: 10/14/07
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Loc: British Columbia
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Re: Germ. time [Re: resptodd]
    #7574379 - 10/29/07 08:34 PM (16 years, 4 months ago)

I am a First time grower but i have been reading these forums for quite a long time. This might be my first post also :blush: he he. But i got 4 different syringes and they all were crystal clear with no traces of spores. Right now i have abut 24 pf jars inoculating and i have only 3 with no myc signs. I also have 2 LCs with obvious growth so i doubt you got a beat syringe, i am sure there is a slim chance though. If you used flame sterilization you could have not let the tip cool?

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OfflineCaribou_Lou
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Registered: 10/17/07
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Re: Germ. time [Re: budzeno]
    #7574394 - 10/29/07 08:37 PM (16 years, 4 months ago)

I thought just shooting some spore solution out is enough to cool the needle? I hope I didn't mess up on the jars I just did a couple days ago

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OfflinePr0_X
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Re: Germ. time [Re: resptodd]
    #7574395 - 10/29/07 08:37 PM (16 years, 4 months ago)

Seven day's is a long time to see growth, depending on what total jar temperature is at 72f to 80f being optimum to see growth in hydrated spores in three days would be correct but seeing as they were in your syringe they should have been re-hydrated, could have been cooked in the heat if you didn't catch them in time and they sat in your mail box or could be poor moisture content.

Ive botched a great many jar's of moisture content and had poor spore germination rates but if your on the money it's usually fairly quick, there's really no way to know if it was a bunk syringe less you have a way of testing it, ala, LC or Agar.

IMO Ive had jar's take up to 12 days to see first signs of mycelium but you'll know the syringe was bunk if nothing shows up at all.. and if thats the case report it to your vendor I'm sure they'll replace it.


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It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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Offlineresptodd
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Re: Germ. time [Re: This guy]
    #7574421 - 10/29/07 08:44 PM (16 years, 4 months ago)

yes


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Damn! I'm having fun! Just keep the GD monkeys away.

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Offlineresptodd
I reject yourreality andsubstitute myown
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Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 month, 14 days
Re: Germ. time [Re: resptodd]
    #7574434 - 10/29/07 08:47 PM (16 years, 4 months ago)

So, respectfully, I ask again. What is the average time in which I should see growth in the jars?


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Damn! I'm having fun! Just keep the GD monkeys away.

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Offlinebudzeno
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Registered: 10/14/07
Posts: 225
Loc: British Columbia
Last seen: 11 years, 1 month
Re: Germ. time [Re: resptodd]
    #7574451 - 10/29/07 08:52 PM (16 years, 4 months ago)

Well I am at 10 days now and i have 2 jars 85% and 3 with none and the rest in the middle. I saw first signs of growth at about 4 days. I read about people waiting a long time though.

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OfflinePr0_X
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Re: Germ. time [Re: budzeno]
    #7574574 - 10/29/07 09:25 PM (16 years, 4 months ago)

Between three and ten days.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl

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Offlineveda_sticks
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Re: Germ. time [Re: Pr0_X]
    #7575475 - 10/30/07 05:47 AM (16 years, 4 months ago)

Did you get your spores from a shroomery sponsor??

If not, its possible u have a bunk syringe, but still wait it out and see what happens. Spores are microscopic, when u see clumps of spores your seeing thousands of thousands of the things.

As long as your substrate moisture content is right, u sterilised right, allowed enough time for them to cool, and incoculated in sterile fashion like in a glovebox, flamed the needle cooled it down (squirting a little solution out is fine, but i just use and alcohol swab so to not waste spores) then everything should be fione


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
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Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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