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OfflineCheesekiller
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First LC
    #7515159 - 10/14/07 11:46 AM (14 years, 1 month ago)

My friend gave me some syringes. One was a Koh Samui super strain. Problem was that one only had about 2 CC's left. So I decided to make a LC. I used about two tablespoons of light karo corn syrup, in about 12 oz of water. I used one of the same lids from my grain jars.



PC for 20 min @15PSI. Flame sterilize the needle and knocked up the cool jar. I have had it incubating at 80F for about 5 days now.




Questions.
1)Is it all done.

2) when I use it to knock up my grain jars do I have to make sure and get a bunch of that cloud in my syringe?

3) Is it bad to shake it up at this time?


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OfflineXeluc
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Re: First LC [Re: Cheesekiller]
    #7515356 - 10/14/07 01:08 PM (14 years, 1 month ago)

1) Looks good to me.
2) Sure, although if there's any VISIBLE mycelium in the syringe you use, you have plenty, I wouldn't worry too much about it
3)nope! I would, although like #2, it doesn't really matter as long as you end up with myc in the syringe, it wont hurt it to shake though.


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OfflineCheesekiller
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Re: First LC [Re: Xeluc]
    #7515413 - 10/14/07 01:27 PM (14 years, 1 month ago)

Thanks, I have heard that this Koh Samui is explosive, so this LC should really help out the colonization.

Any tips on getting the stuff into the syringe? I was thinking about using alcohol to sterilize a few empty syringes, maybe fill and drain once or twice?? Then fill up the syringe with the LC? I'm not too sure about that? I could PC the syringes with water, then drain when cold and fill like that??

After that I have the process down pretty good.

-CK


--------------------
Bulk grower with "some" success.

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OfflineXeluc
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Re: First LC [Re: Cheesekiller]
    #7515699 - 10/14/07 02:51 PM (14 years, 1 month ago)

cover syringes in foil, boil or pc them, then load up. :smile: the syringes don't have to be filled when sterilizing, i wouldn't even fill them. As for how to suck it up, just stick your needle in and aim for the cloud of myc! If you have a hard time sucking it up, shake shake shake!


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OfflineFunkatron9000
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Re: First LC [Re: Xeluc]
    #7515835 - 10/14/07 03:29 PM (14 years, 1 month ago)

looking good man, boil some water up and suck it in the syringe acouple times. let it cool and get in there and show that cloud who is boss.


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OfflineCheesekiller
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Re: First LC [Re: Funkatron9000]
    #7516574 - 10/14/07 06:46 PM (14 years, 1 month ago)

Sweet, I'll post pics of this periodically.

-CK


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Offlinejust me
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Re: First LC [Re: Cheesekiller]
    #7516625 - 10/14/07 06:59 PM (14 years, 1 month ago)

in the same boat as you killer, my LC doesnt look as pretty as yours tho. hope you dont mind if i post a pic of it on here, but i have all the same Q's as you, and just need someone to tell me "its ok" haha

thanks for starting this thread tho, all my Q's other than is my LC ok were answered!



thanks, just me


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Invisiblemushbaby
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Re: First LC [Re: just me]
    #7517219 - 10/14/07 09:39 PM (14 years, 1 month ago)

Y'all meant stir not shake correct? Try not to get the filter patch wet. Possible way for contaminants to be introduced to your lc. But it might depend on the filter patch.


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OfflineXeluc
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Re: First LC [Re: mushbaby]
    #7517327 - 10/14/07 10:21 PM (14 years, 1 month ago)

well when i said shake, I was thinking he had a tyvek filter, which repels water (unless the lc is real hot for some reason, then I get small droplets forming) regardless though, "Agitate" your LC :wink:


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Invisiblelarge_dose
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Re: First LC [Re: Xeluc]
    #7517419 - 10/14/07 10:46 PM (14 years, 1 month ago)

From what it looks like you could get at least 3-4 loaded syringes.

I usually aim right for the cloud and try to suck up as much as myc possible. Once im done there is visible myc all the way up the syringe.

I never shake at that stage.

Heres a pic of Karo LC's done the exact procedure as yours 3 weeks in:


If your's have only been colonizing 5 days than thats some aggressive stuff


Looks great to me.:thumbup:


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OfflineCheesekiller
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Re: First LC [Re: large_dose]
    #7528731 - 10/17/07 07:50 PM (14 years, 1 month ago)

Getting the stuff into the syringe was tough. It snotted up when trying to aspirate, so I shook. Then I got floaties in the syringes. I blasted 7 QTs WBS with the stuff yesterday, and threw them in the incubator. There is a little activity today but nothing to post pics of yet.

Next time I'm gonna use a real low pro jar for the LC, so I can get my syringe in there a little easier (without having to tilt it on its side).

Wish you all luck. I'm betting 6 days to full colonization. I have to get my poo ready soon!! Any bets??

-CK


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OfflineMepher
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Re: First LC [Re: Cheesekiller]
    #7529861 - 10/18/07 03:34 AM (14 years, 1 month ago)

Looks like you're on the right path, Cheesekiller. It can take a few days to see growth, but your jars will go WAY faster with the LC than using ungerminated spores. Good luck!


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OfflineCheesekiller
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CONTAMINATED [Re: Mepher]
    #7552178 - 10/23/07 08:40 PM (14 years, 1 month ago)

:frown:

I think when I shook up the LC I got contams somehow.  All of the 7 qt jars had white shit all over them after 2 days.  The I noticed green SHIT.

Another error that may have caused (probably) was I forgot to shake the jars after PC'ing.  So the next day I sterilized a knife, broke the jars open, and agitated with the knife.  This could have sucked in contams. 

This worked for me in the past when I forgot that crucial step (shaking after PCing) with no contamination, so the only other source  would have been the original syringe. I got it with only 2CC's of left over from a friend. 

Question. 

Wouldn't I have noticed contams in the LC??  It looked like the lid had some crap on the inside of the tyvek patch.  I figured from shaking it up. 

Does the LC actually need air exchange?

With that I just PC'ed 7 more qts with WBS, and have some fresh home made muli-spore Amazonian Cube syringes.  Never fails.  This is not the last attempt at an LC however.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup


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OfflineVisionsToReality
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Re: CONTAMINATED [Re: Cheesekiller]
    #7552269 - 10/23/07 08:59 PM (14 years, 1 month ago)

Ive read LCs do better with air exchange but its not essential
and that contams are hard to notice in LC until you inoc grains and then it becomes evident (a downside of LC, whereas w/ g2g this is not the case)


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Invisibledjsollest
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Re: CONTAMINATED [Re: Cheesekiller]
    #7552293 - 10/23/07 09:04 PM (14 years, 1 month ago)

Hey man all my LC's have turned out JUST like yours and I had the exact same thing happen in my brf jars after inoc with the LC . They all had nice white growth in a day or two then like day 3-4 I saw little green dots EVERYWHERE!?!? Whats the deal with this? And what do you mean "shaking after PCing jars"
Ive never heard of this, could you explain it?


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Offlinecroptop30
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Re: CONTAMINATED [Re: djsollest]
    #7552368 - 10/23/07 09:20 PM (14 years, 1 month ago)

I never use an air hole I use baby bottles with RTV sealant in the nipple and I throw a marble in so you can stir it. you don't need alot of fresh air it will grow without an air hole. let it grow in a dark closet for about a week or two and then throw it in the frigerator from then out it will stay fresh for along time.


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Offlineprimordialoddity
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Re: CONTAMINATED [Re: croptop30]
    #7553257 - 10/24/07 01:13 AM (14 years, 1 month ago)

I shot up 2 quarts with 2-3ml of B+ spores in 500ml malt/dex...

it has been 2.5 days and I see no growth, good or bad... Thought i'd have visible myc by now, but I'll wait a week then try again...

I made my own stirrer...I;m itchn to use it!


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Offlineveda_sticks
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Re: CONTAMINATED [Re: primordialoddity]
    #7553682 - 10/24/07 06:31 AM (14 years, 1 month ago)

its takes 3-4 days for the spores to germinate, wait longer.


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OfflineCheesekiller
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Re: CONTAMINATED [Re: djsollest]
    #7554364 - 10/24/07 12:17 PM (14 years, 1 month ago)

Quote:

djsollest said:
And what do you mean "shaking after PCing jars"
Ive never heard of this, could you explain it?




This is because if you are using grains like WBS in particular, the grains get all clotted together after pressure cooking. If you shake them about an hour after PCing then the grains break up easily. In this case I had forgotten, then used a sterilized knife to break them up. I really think my contamination came from the LC though. Next time I will try Agars tek with the baby bottles etc.

-CK


--------------------
Bulk grower with "some" success.

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Nice Lids

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OfflineMotorCityMadman
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Re: CONTAMINATED [Re: Cheesekiller]
    #7555068 - 10/24/07 03:53 PM (14 years, 1 month ago)

Personally I've had way more contams with LCs than anything else. I'm strictly g2g at this point. Only reason I would ever do LC is if I were trying to knock more jars than would fit in my glove box.


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