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Offlineanthony034
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Registered: 05/24/07
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Liquid culture question
    #7412775 - 09/15/07 11:04 AM (16 years, 6 months ago)

have a karo jar of spore inoculated LC. are there any definite ways to tell you have a contam?


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Offlinegrapejelly

Registered: 08/15/07
Posts: 268
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Re: Liquid culture question [Re: anthony034]
    #7412822 - 09/15/07 11:23 AM (16 years, 6 months ago)

I think if it's brown (or any color besides white) and sinks to the bottom you have a contam. Other than that, you just have to knock up a test jar or two to find out.

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OfflineCaptainLinger
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Registered: 05/25/07
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Re: Liquid culture question [Re: grapejelly]
    #7413905 - 09/15/07 05:07 PM (16 years, 6 months ago)

Quote:

I think if it's brown (or any color besides white) and sinks to the bottom you have a contam.




That doesn't make a lot of sense. Mycelium will often sink to the bottom. If you're talking to a noob, the carmelization (which will appear golden brown) of sugars tinting the white mycelium will be mistaken for your (?) brown contams.

You can't tell visually. You'll get to know the range of what they look like, but until you do, just knock up jars.

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InvisibleBlutjager
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Re: Liquid culture question [Re: CaptainLinger]
    #7414122 - 09/15/07 06:28 PM (16 years, 6 months ago)

Quote:

CaptainLinger said:You'll get to know the range of what they look like, but until you do, just knock up jars.




Generally I think more good lc gets thrown away due sediment being mistaken for contamination than bad lc gets used to knock up jars

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OfflineDr_T
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Re: Liquid culture question [Re: Blutjager]
    #7414305 - 09/15/07 07:42 PM (16 years, 6 months ago)

Shoot up a jar of grain, you'll know in a couple of days.


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Offlineanthony034
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Re: Liquid culture question [Re: grapejelly]
    #7415675 - 09/16/07 07:12 AM (16 years, 6 months ago)

Quote:

cupcake said:
I think if it's brown (or any color besides white) and sinks to the bottom you have a contam. Other than that, you just have to knock up a test jar or two to find out.




it is sinking to the bottom, but looks filmy and white. i shot up some 250ml jars as a test.


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Offlinemushyflushy
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Registered: 09/13/07
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Re: Liquid culture question [Re: anthony034]
    #7415966 - 09/16/07 08:59 AM (16 years, 6 months ago)

yes sinking powder to the bottom and if the jar looks cloudy and looses the clean look


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Offlinemushyflushy
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Registered: 09/13/07
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Re: Liquid culture question [Re: mushyflushy]
    #7415967 - 09/16/07 08:59 AM (16 years, 6 months ago)

good luck


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Offlinegrapejelly

Registered: 08/15/07
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Re: Liquid culture question [Re: mushyflushy]
    #7416171 - 09/16/07 10:41 AM (16 years, 6 months ago)

I wasn't talking about carmelization, I was talking about actual contams, which was what the OP originally asked about. Of course, it very well could be carmelization or sediment. Here is more info about CONTAMS, by Wronguy.


Quote:

Wronguy said:
It depends on the contaminate, but usually it looks brown and will typically stay near the bottom. Mycelia will typically be hindered in growth and will fall to the bottom and die, if it grows at all. What you want is a nice, white fluffy substance that will be suspended inside the LC, not laying on the bottom.



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OfflineCaptainLinger
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Registered: 05/25/07
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Re: Liquid culture question [Re: grapejelly]
    #7418624 - 09/17/07 12:43 AM (16 years, 6 months ago)

My point is that your description yields no distinction. Mycelium also frequently sinks to the bottom. Good LC can appear brown. Furthermore, contaminants are unlikely to appear as anything but white. My last PE liquid culture a while back, despite being properly sterilized, and cooked with little carmelization, developed a small cloud, turned brown, and sunk to the bottom. Weird black rods (unknown origin, but floating amongst the mycelium) showed up too. I garnered very respectable yields on coir after spawning to WBS. My latest king oyster LC, a generous donation by another member, grew amply and stayed clear. Looked great, until I knocked up jars, and each and every one of the 20 contaminated. My mushroom-selling business goes not so well :frown:.

My point is not that your direction is wrong, really, and I'm sorry if I was presumptuous. It's just that it's never worth throwing away an LC based on visual identification. Metabolites, inert jar material, carmelization, etc, can all throw off false signs. The real answer is...buck up, PC a single jar, draw a syringe, and try. There's never any reason to throw out LC until it fails.

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