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InvisibleLayYouIn
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Sterilize Jar For Print In Oven?
    #7333857 - 08/25/07 05:54 PM (16 years, 7 months ago)

if i have a jar that has 7 holes in the top of it, with polyester fiber in those holes, and tin foil wrap all around it, can i sterilize it in the over to prepare for a spore print?

if yes, what temp and how long?

thanks.

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Invisiblebudmanman
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7333891 - 08/25/07 06:01 PM (16 years, 7 months ago)

I would pressure cook it my self and have no holes in the lid.


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Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: budmanman]
    #7333991 - 08/25/07 06:32 PM (16 years, 7 months ago)

Quote:

budmanman said:
I would pressure cook it my self and have no holes in the lid.




could i use an oven though? it would make it a lot easier and i dont see why it wouldn't work...

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Invisiblebudmanman
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7334003 - 08/25/07 06:35 PM (16 years, 7 months ago)

well with a pc you KNOW it is sterile, no doubt about it.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: budmanman]
    #7334012 - 08/25/07 06:37 PM (16 years, 7 months ago)

Quote:

budmanman said:
well with a pc you KNOW it is sterile, no doubt about it.




would an oven not work?

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Invisibledysphoria
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Re: Sterilize Jar For Print In Oven? [Re: budmanman]
    #7334015 - 08/25/07 06:38 PM (16 years, 7 months ago)

yes, oven is fine for sterilization of items that do not require moisture and which do not melt.

in other words, aluminum and glass and metal, etc.

afterall, an oven can get far hotter than a pc can.


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InvisiblePremedman1
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7334018 - 08/25/07 06:38 PM (16 years, 7 months ago)

Quote:

LayYouIn said:
Quote:

budmanman said:
well with a pc you KNOW it is sterile, no doubt about it.




would an oven not work?




Sure it would.


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Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.

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Invisiblebudmanman
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Re: Sterilize Jar For Print In Oven? [Re: Premedman1]
    #7334024 - 08/25/07 06:40 PM (16 years, 7 months ago)

Dont get the jars to hot and then let them cool to fast!


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: dysphoria]
    #7334025 - 08/25/07 06:40 PM (16 years, 7 months ago)

would polyester fiber melt?

what temp should i do this at?

for how long?

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Invisiblemusher_420
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7334083 - 08/25/07 07:05 PM (16 years, 7 months ago)

From here
http://textbookofbacteriology.net/control.html

Methods of Sterilization

Heat: most important and widely used. For sterilization always consider type of heat, time of application and temperature to ensure destruction of all microorganisms. Endospores of bacteria are considered the most thermoduric of all cells so their destruction guarantees sterility.

Incineration: burns organisms and physically destroys them. Used for needles , inoculating wires, glassware, etc. and objects not destroyed in the incineration process.

Boiling: 100o for 30 minutes. Kills everything except some endospores (Actually, for the purposes of purifying drinking water 100o for five minutes is probably adequate though there have been some reports that Giardia cysts can survive this process). To kill endospores, and therefore sterilize the solution, very long or intermittent boiling is required.

Autoclaving (steam under pressure or pressure cooker): 121o for 15 minutes (15#/in2 pressure). Good for sterilizing almost anything, but heat-labile substances will be denatured or destroyed.

Dry heat (hot air oven): 160o/2hours or 170o/1hour. Used for glassware, metal, and objects that won't melt.

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InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: musher_420]
    #7334158 - 08/25/07 07:33 PM (16 years, 7 months ago)

Quote:

musher_420 said:
170o/1hour. Used for glassware, metal, and objects that won't melt.




that's 170f?

btw, nice link...

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Invisiblemycocurious
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7334517 - 08/25/07 09:33 PM (16 years, 7 months ago)

Personally, I've done nothing more than...

1. clean thoroughly with soap and water.
2. boil for 20-30 minutes
3. profit.

Sterilizing equipment is easiest sterilized in boiling water. Sterilizing food sources (substrates, grains, pf-styled jars.) is a different story altogether and should be done in a PC/autoclave.


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.

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InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7337717 - 08/26/07 07:52 PM (16 years, 7 months ago)

so what temp and for how long?

is it 170f for 1 hour?  :confused:

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InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7341375 - 08/27/07 06:01 PM (16 years, 7 months ago)

270f for 30 minutes?

will the rubber on the jar melt? will the polyester fiber melt?

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InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7341671 - 08/27/07 07:20 PM (16 years, 7 months ago)

Quote:

LayYouIn said:
270f for 30 minutes?

will the rubber on the jar melt? will the polyester fiber melt?




does anyone know?

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Invisiblemycocurious
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7341720 - 08/27/07 07:32 PM (16 years, 7 months ago)

once again, boiling it in a pot of water to sterilize the empty jars would be _the_ safest way to go here.


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.

Extras: Filter Print Post Top
InvisibleLayYouIn
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Re: Sterilize Jar For Print In Oven? [Re: mycocurious]
    #7341742 - 08/27/07 07:36 PM (16 years, 7 months ago)

Quote:

mycocurious said:
once again, boiling it in a pot of water to sterilize the empty jars would be _the_ safest way to go here.




fine.  :crankey:

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Invisiblemycocurious
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Re: Sterilize Jar For Print In Oven? [Re: LayYouIn]
    #7341823 - 08/27/07 07:53 PM (16 years, 7 months ago)

you could put it in the oven, but we're talking about glass and anything outside a 212(F) water bath makes me worried it would shatter.

Even when boiling it, it can shatter. Make sure you start it off in cold water, bring that to a slow boil and then take the pot of the heat and let it cool slowly.

I'm overly paranoid about a shattering glass after I've seen it embedded into cabinets.


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.

Extras: Filter Print Post Top
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