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Offlinebrodus24
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Amazing Isolate EATS contams!!!
    #7301685 - 08/16/07 04:53 PM (16 years, 7 months ago)

This is the coolest thing that I've happened upon in this hobby, next to my shroom-on-shroom grow.

I have a PB (Thai Pink Buffalo) strain that I isolated from the largest, fastest growing fruit. This was a veil/cap tissue isolation in bacteriostatic water.

Anyhow...its INSANE...I've had two WBS jars now that initially contaminated a bit...in both cases the PB myc overtook the contams, spitting out a great deal of metabolites (myco-piss) along the way. First jar had pink mold...this current jar had green/gray trich. that was evident upon shaking...now its almost all gone and covered with healthy white Mycelium!

I'm taking photos to post, because its unbelievable...

FYI, I had other jars that had no contams. These jars were jars that had initially been inoculated and for whatever reason, they never spawned mycelium....so I tossed in some PB-isolate liquid, and within a couple days had mycelium...then some contams and mycelium...and now ONLY mycelium!

I'm going to clone this isolate forever!

ON a side note, I just watched a YouTube video with Paul Stamets explaining mushroom-based bio-remediation, and if mycelium can eat and digest oil slicks, it makes sense that they can overtake contams.

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OfflineDr_T
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301734 - 08/16/07 05:12 PM (16 years, 7 months ago)

Nice!

(Could I get a sample? Please?)


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.

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Offlinedeformedreality
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301757 - 08/16/07 05:22 PM (16 years, 7 months ago)

too bad that its frowned on to send myc lc through the mail to other members.. id probally even pay for it... COUGH COUGH COUGH


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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: deformedreality]
    #7301842 - 08/16/07 05:59 PM (16 years, 7 months ago)

Yeah, its going to stay in my collection forever I think :smile:

I'm slowly going all-clone/isolate...this is how some people have their WBS jars colonize in 14-18 days, while others take 30+.

I've had cakes of the same multi-spore produce shat, or produce huge.  Its all because I didn't have an isolate.

I think my new strategy for each new strain is:
1. FIRST test the strain on BRF...isolate the best, make an LC of that
2. THEN grow out the iso-LC on WBS to poo/straw.
3. Repeat forever until I have 5 or so strains that represent 5 or so different characteristics I look for (size, colonization speed, etc.)

In this manner, I'm never out on a shitty casing, slug-slow WBS colonization, or sub-par flush.

I took some photos, but the light doesn't show the digested trich very well...I'll try to find another way to photograph.

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Offlinexaxphaanes
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301898 - 08/16/07 06:12 PM (16 years, 7 months ago)

not to rain on your parade but all that happened is the mycelium covered up the contam, the contam is still there if you spawn i almost guarantee that it will contam i have had this happen a few times.


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"Anything i say is fictional"
  what you should look for in manure

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Offlinedeformedreality
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301924 - 08/16/07 06:20 PM (16 years, 7 months ago)

well im just glad i dont have to rely on a myc that can get rid of contams.. hell i can basically wipe my ass with cakes and they wont contam..


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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
    #7302097 - 08/16/07 07:16 PM (16 years, 7 months ago)

Actually, the first jar is already cased in CC and 7 days done--just looked at it: zero contams, just a ton of fluffy white mycelium.

I'll post results on everything, but its not at all unlikely that the contams we overtaken. Ref: myco-remediation.

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Offlinexaxphaanes
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7302181 - 08/16/07 07:38 PM (16 years, 7 months ago)

ok well gl out of maybe 100 jars that i tried ended up with contams.


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"Anything i say is fictional"
  what you should look for in manure

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Invisiblejeetered
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
    #7302244 - 08/16/07 07:59 PM (16 years, 7 months ago)

ok i've searched every term possible on youtube for the video you refer to... don't find it. can we get a link? i've read it but not seen it.

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Invisiblejeetered
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Re: Amazing Isolate EATS contams!!! [Re: jeetered]
    #7302264 - 08/16/07 08:04 PM (16 years, 7 months ago)


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Offlinedeformedreality
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7302280 - 08/16/07 08:11 PM (16 years, 7 months ago)

the question is, are the oysters still edible...


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Offlinexaxphaanes
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Re: Amazing Isolate EATS contams!!! [Re: jeetered]
    #7302302 - 08/16/07 08:16 PM (16 years, 7 months ago)

yes that video is pretty good..also check out anastomosis which is basically the joining of two diff mycelia i.e green mold/cobweb etc etc


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"Anything i say is fictional"
  what you should look for in manure

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Offlineimplee
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
    #7302363 - 08/16/07 08:32 PM (16 years, 7 months ago)

Mushrooms are hardcore!

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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: implee]
    #7305056 - 08/17/07 01:56 PM (16 years, 7 months ago)

Just search Paul Stamets on YouTube...there's a lot of good stuff...he's my hero

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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7386501 - 09/09/07 01:16 AM (16 years, 6 months ago)

FYI, this casing is fruiting now and I have an insane amount of pins...I'll post photos.

You won't believe it.

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OfflineThe shroomy 1
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Re: Amazing Isolate EATS contams!!! [Re: deformedreality]
    #7386514 - 09/09/07 01:25 AM (16 years, 6 months ago)

Quote:

deformedreality said:
well im just glad i dont have to rely on a myc that can get rid of contams.. hell i can basically wipe my ass with cakes and they wont contam..




SHHHHHHHHHHHHHH!!!!!!!!!!! The contamination GODS will hear you!!


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AMU Q&A thread.

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OfflineeVenom
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Re: Amazing Isolate EATS contams!!! [Re: The shroomy 1]
    #7388115 - 09/09/07 12:59 PM (16 years, 6 months ago)

come one almost a month and still no pictures I'm dying to see them


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eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR

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InvisibleWeed_Indicated
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Re: Amazing Isolate EATS contams!!! [Re: deformedreality]
    #7388254 - 09/09/07 01:51 PM (16 years, 6 months ago)

Quote:

deformedreality said:
COUGH COUGH COUGH




LOL


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FrankHorrigan WBS Prep
Magesh's Rye Tek
SixTango's G2G Tek
Liquid Culture Basics
MonsterMitch's Monotub Tek

----
No statements made in any post or message by myself should be understood to mean that I choose to participate in activities which break federal, state, or local laws. All posts are strictly fictional.

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Offlineveda_sticks
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Re: Amazing Isolate EATS contams!!! [Re: Weed_Indicated]
    #7388296 - 09/09/07 02:06 PM (16 years, 6 months ago)

interesting


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: veda_sticks]
    #7393212 - 09/10/07 06:50 PM (16 years, 6 months ago)

Here are photos--was waiting to get my good camera back, as the cellphone just doesn't do justice.

Got some early poppers, but below them a very dense pinset.

Beware: these are LARGE:







Edited by brodus24 (09/10/07 06:51 PM)

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