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brodus24
Fruit of theBoomer
Registered: 03/21/07
Posts: 128
Last seen: 10 years, 8 months
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Amazing Isolate EATS contams!!!
#7301685 - 08/16/07 04:53 PM (16 years, 7 months ago) |
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This is the coolest thing that I've happened upon in this hobby, next to my shroom-on-shroom grow.
I have a PB (Thai Pink Buffalo) strain that I isolated from the largest, fastest growing fruit. This was a veil/cap tissue isolation in bacteriostatic water.
Anyhow...its INSANE...I've had two WBS jars now that initially contaminated a bit...in both cases the PB myc overtook the contams, spitting out a great deal of metabolites (myco-piss) along the way. First jar had pink mold...this current jar had green/gray trich. that was evident upon shaking...now its almost all gone and covered with healthy white Mycelium!
I'm taking photos to post, because its unbelievable...
FYI, I had other jars that had no contams. These jars were jars that had initially been inoculated and for whatever reason, they never spawned mycelium....so I tossed in some PB-isolate liquid, and within a couple days had mycelium...then some contams and mycelium...and now ONLY mycelium!
I'm going to clone this isolate forever!
ON a side note, I just watched a YouTube video with Paul Stamets explaining mushroom-based bio-remediation, and if mycelium can eat and digest oil slicks, it makes sense that they can overtake contams.
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Dr_T
Random Dude
Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 5 months
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
#7301734 - 08/16/07 05:12 PM (16 years, 7 months ago) |
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Nice!
(Could I get a sample? Please?)
-------------------- Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.
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deformedreality
Stranger
Registered: 06/16/07
Posts: 2,190
Last seen: 4 years, 9 months
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
#7301757 - 08/16/07 05:22 PM (16 years, 7 months ago) |
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too bad that its frowned on to send myc lc through the mail to other members.. id probally even pay for it... COUGH COUGH COUGH
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brodus24
Fruit of theBoomer
Registered: 03/21/07
Posts: 128
Last seen: 10 years, 8 months
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Yeah, its going to stay in my collection forever I think
I'm slowly going all-clone/isolate...this is how some people have their WBS jars colonize in 14-18 days, while others take 30+.
I've had cakes of the same multi-spore produce shat, or produce huge. Its all because I didn't have an isolate.
I think my new strategy for each new strain is: 1. FIRST test the strain on BRF...isolate the best, make an LC of that 2. THEN grow out the iso-LC on WBS to poo/straw. 3. Repeat forever until I have 5 or so strains that represent 5 or so different characteristics I look for (size, colonization speed, etc.)
In this manner, I'm never out on a shitty casing, slug-slow WBS colonization, or sub-par flush.
I took some photos, but the light doesn't show the digested trich very well...I'll try to find another way to photograph.
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xaxphaanes
Mycologist
Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 7 months
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
#7301898 - 08/16/07 06:12 PM (16 years, 7 months ago) |
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not to rain on your parade but all that happened is the mycelium covered up the contam, the contam is still there if you spawn i almost guarantee that it will contam i have had this happen a few times.
-------------------- "Anything i say is fictional" what you should look for in manure
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deformedreality
Stranger
Registered: 06/16/07
Posts: 2,190
Last seen: 4 years, 9 months
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
#7301924 - 08/16/07 06:20 PM (16 years, 7 months ago) |
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well im just glad i dont have to rely on a myc that can get rid of contams.. hell i can basically wipe my ass with cakes and they wont contam..
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brodus24
Fruit of theBoomer
Registered: 03/21/07
Posts: 128
Last seen: 10 years, 8 months
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
#7302097 - 08/16/07 07:16 PM (16 years, 7 months ago) |
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Actually, the first jar is already cased in CC and 7 days done--just looked at it: zero contams, just a ton of fluffy white mycelium.
I'll post results on everything, but its not at all unlikely that the contams we overtaken. Ref: myco-remediation.
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xaxphaanes
Mycologist
Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 7 months
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
#7302181 - 08/16/07 07:38 PM (16 years, 7 months ago) |
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ok well gl out of maybe 100 jars that i tried ended up with contams.
-------------------- "Anything i say is fictional" what you should look for in manure
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jeetered
Stranger
Registered: 07/07/06
Posts: 3,055
Loc: no clue
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
#7302244 - 08/16/07 07:59 PM (16 years, 7 months ago) |
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ok i've searched every term possible on youtube for the video you refer to... don't find it. can we get a link? i've read it but not seen it.
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jeetered
Stranger
Registered: 07/07/06
Posts: 3,055
Loc: no clue
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Re: Amazing Isolate EATS contams!!! [Re: jeetered]
#7302264 - 08/16/07 08:04 PM (16 years, 7 months ago) |
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deformedreality
Stranger
Registered: 06/16/07
Posts: 2,190
Last seen: 4 years, 9 months
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
#7302280 - 08/16/07 08:11 PM (16 years, 7 months ago) |
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the question is, are the oysters still edible...
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xaxphaanes
Mycologist
Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 7 months
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Re: Amazing Isolate EATS contams!!! [Re: jeetered]
#7302302 - 08/16/07 08:16 PM (16 years, 7 months ago) |
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yes that video is pretty good..also check out anastomosis which is basically the joining of two diff mycelia i.e green mold/cobweb etc etc
-------------------- "Anything i say is fictional" what you should look for in manure
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implee
Cyber Hippie
Registered: 07/27/06
Posts: 5,833
Loc: Houston, Texas.
Last seen: 7 months, 7 days
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
#7302363 - 08/16/07 08:32 PM (16 years, 7 months ago) |
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Mushrooms are hardcore!
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brodus24
Fruit of theBoomer
Registered: 03/21/07
Posts: 128
Last seen: 10 years, 8 months
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Re: Amazing Isolate EATS contams!!! [Re: implee]
#7305056 - 08/17/07 01:56 PM (16 years, 7 months ago) |
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Just search Paul Stamets on YouTube...there's a lot of good stuff...he's my hero
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brodus24
Fruit of theBoomer
Registered: 03/21/07
Posts: 128
Last seen: 10 years, 8 months
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
#7386501 - 09/09/07 01:16 AM (16 years, 6 months ago) |
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FYI, this casing is fruiting now and I have an insane amount of pins...I'll post photos.
You won't believe it.
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The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 6 months, 24 days
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Quote:
deformedreality said: well im just glad i dont have to rely on a myc that can get rid of contams.. hell i can basically wipe my ass with cakes and they wont contam..
SHHHHHHHHHHHHHH!!!!!!!!!!! The contamination GODS will hear you!!
-------------------- AMU Q&A thread.
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eVenom
DRIFT on toBATTLE
Registered: 08/25/07
Posts: 869
Loc: Florida
Last seen: 6 months, 1 day
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Re: Amazing Isolate EATS contams!!! [Re: The shroomy 1]
#7388115 - 09/09/07 12:59 PM (16 years, 6 months ago) |
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come one almost a month and still no pictures I'm dying to see them
-------------------- eVenom "Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god." RR
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Weed_Indicated
Hemp will Savethe Planet
Registered: 06/04/07
Posts: 157
Loc: FEMA Camp #420
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Quote:
deformedreality said: COUGH COUGH COUGH
LOL
-------------------- FrankHorrigan WBS Prep Magesh's Rye Tek SixTango's G2G Tek Liquid Culture Basics MonsterMitch's Monotub Tek ---- No statements made in any post or message by myself should be understood to mean that I choose to participate in activities which break federal, state, or local laws. All posts are strictly fictional.
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: Amazing Isolate EATS contams!!! [Re: Weed_Indicated]
#7388296 - 09/09/07 02:06 PM (16 years, 6 months ago) |
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interesting
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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brodus24
Fruit of theBoomer
Registered: 03/21/07
Posts: 128
Last seen: 10 years, 8 months
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Re: Amazing Isolate EATS contams!!! [Re: veda_sticks]
#7393212 - 09/10/07 06:50 PM (16 years, 6 months ago) |
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Here are photos--was waiting to get my good camera back, as the cellphone just doesn't do justice.
Got some early poppers, but below them a very dense pinset.
Beware: these are LARGE:
Edited by brodus24 (09/10/07 06:51 PM)
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