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BlufNBrent
The Man
Registered: 04/24/07
Posts: 177
Last seen: 16 years, 3 months
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How many CC's can come of 1 spore print?
#7047029 - 06/14/07 05:47 PM (16 years, 9 months ago) |
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Planning on doing Darkwing Syringe Tek, put cap in yogurt container, let spores drop, add sterile water. Was just wondering if anyone knew how many 10cc syringes I could fill off of this print.
I would like to fill the yogurt container completly full of sterile water on top of this one print, and fill as many syringes as I can with it.
But, I'm affraid that my spore ratio to water might give me some syringes full of water, with no spores.
How many 10cc syringes could I fill off a 1" GT spore print, and still have sufficient syringes?
Any help, is greatly appreciated.
-------------------- Just 4 Laughs, I would not in anyway break state, local or federal laws.
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jeetered
Stranger
Registered: 07/07/06
Posts: 3,055
Loc: no clue
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Re: How many CC's can come of 1 spore print? [Re: BlufNBrent]
#7047035 - 06/14/07 05:49 PM (16 years, 9 months ago) |
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a decent print could yeild up to 30 syringes, if you're using 10cc syringes, and those would be pretty dark syringes.
The syringes i get from ralphsters are pretty freekin dark, and i could make one of those into 3.
:P
Edited by jeetered (06/14/07 05:50 PM)
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CaptTrip
Captain
Registered: 01/17/07
Posts: 265
Last seen: 15 years, 1 month
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Re: How many CC's can come of 1 spore print? [Re: BlufNBrent]
#7047036 - 06/14/07 05:50 PM (16 years, 9 months ago) |
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Just make a normal spore syringe, then use that to create a liquid culture...Then you can create enough LC to make hundreds of syringes.
-------------------- Cambodian Grow Log
Edited by CaptTrip (06/14/07 05:54 PM)
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BlufNBrent
The Man
Registered: 04/24/07
Posts: 177
Last seen: 16 years, 3 months
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Re: How many CC's can come of 1 spore print? [Re: CaptTrip]
#7047080 - 06/14/07 06:10 PM (16 years, 9 months ago) |
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Ya, my last LC failed and I'm affraid the one I have in the closet right now is going to fail,(its been 4 days)no sign of anything in it.
Since I suck at LC's and my last bunch of shrooms are about to break vails I need a plan incase my LC fails.
-------------------- Just 4 Laughs, I would not in anyway break state, local or federal laws.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: How many CC's can come of 1 spore print? [Re: BlufNBrent]
#7047096 - 06/14/07 06:17 PM (16 years, 9 months ago) |
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Asking how many cc's you can get from a print is like asking how many birds you can put in a cage. It depends on how big the cage is.
An average sized cap, 2" in diameter will yield five to six 10ml syringes. A much larger cap will give more. Remember, a 4" diameter cap is four times the size of a 2" diameter cap. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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BlufNBrent
The Man
Registered: 04/24/07
Posts: 177
Last seen: 16 years, 3 months
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Re: How many CC's can come of 1 spore print? [Re: RogerRabbit]
#7047109 - 06/14/07 06:24 PM (16 years, 9 months ago) |
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As noted in my original post the "cage" is 1" in diameter. Thank you for the quick reply, I'll asume 3 syringes.
SHROOMERY ROCKS!!!!!
-------------------- Just 4 Laughs, I would not in anyway break state, local or federal laws.
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makaveli8x8
Stranger
Registered: 02/28/06
Posts: 21,636
Last seen: 7 years, 9 months
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Re: How many CC's can come of 1 spore print? [Re: BlufNBrent]
#7047112 - 06/14/07 06:25 PM (16 years, 9 months ago) |
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well there are like what a billion spores on a print? if you could manage one spore per syringe....
-------------------- We were sent to hell for eternity Øh® We play on earth to pass the time Over-population the root of all Evil-brings the Elites Closer to the gates.
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boomerman
psychobilly
Registered: 02/21/06
Posts: 294
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Re: How many CC's can come of 1 spore print? [Re: makaveli8x8]
#7047217 - 06/14/07 06:56 PM (16 years, 9 months ago) |
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Quote:
makaveli8x8 said: well there are like what a billion spores on a print? if you could manage one spore per syringe....
dont you need 2 to germanate ?
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BlufNBrent
The Man
Registered: 04/24/07
Posts: 177
Last seen: 16 years, 3 months
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Re: How many CC's can come of 1 spore print? [Re: boomerman]
#7054121 - 06/16/07 08:50 PM (16 years, 9 months ago) |
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That one spore would come out in the 1st cc, then any other jar I inacculated with that syringe would just be water. Correct?
-------------------- Just 4 Laughs, I would not in anyway break state, local or federal laws.
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 15 years, 6 months
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Re: How many CC's can come of 1 spore print? [Re: BlufNBrent]
#7054167 - 06/16/07 09:03 PM (16 years, 9 months ago) |
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Haha.
Anyway. Yes, let's get on this project with our electron microscopes.
Even though it's a joke, it wouldn't work--probably 99% of the spores injected are genetically inferior in some way or another, and don't wind up becoming dominant. This is why a petri dish with 10,000 spores on it might only make five or ten sectors.
Do you think IVF is done with one sperm?
-------------------- FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 15 years, 6 months
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Re: How many CC's can come of 1 spore print? [Re: boomerman]
#7054191 - 06/16/07 09:09 PM (16 years, 9 months ago) |
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Quote:
boomerman said:
Quote:
makaveli8x8 said: well there are like what a billion spores on a print? if you could manage one spore per syringe....
dont you need 2 to germanate ?
Yes, spores and their hyphae are haploid until they meet and their cells merge.
http://en.wikipedia.org/wiki/Basidiomycota for more general info on the process.
Edit: Said the nuclei merge, which they usually don't, since mushrooms are dikaryotic.
-------------------- FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
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MagicMaker
The Lizard King
Registered: 10/10/06
Posts: 626
Loc: Terrapin
Last seen: 5 years, 11 months
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Re: How many CC's can come of 1 spore print? [Re: RogerRabbit]
#10896985 - 08/20/09 11:40 AM (14 years, 7 months ago) |
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roger rabbit i know this is a super old post but wanted to help your math out and let you know that a 4in diam cap is more then 2x a 2in diam cap http://www.mathgoodies.com/lessons/vol2/circle_area.html
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badman
Registered: 06/14/06
Posts: 4,039
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Re: How many CC's can come of 1 spore print? [Re: MagicMaker]
#10897026 - 08/20/09 11:48 AM (14 years, 7 months ago) |
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Quote:
MagicMaker said: roger rabbit i know this is a super old post but wanted to help your math out and let you know that a 4in diam cap is more then 2x a 2in diam cap http://www.mathgoodies.com/lessons/vol2/circle_area.html
Read his post again and check out
Edited by badman (08/20/09 11:50 AM)
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botanisthype
P$ylocibe$ounDW@vE!
Registered: 07/31/03
Posts: 839
Loc: DECEPTICON MANOR
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Re: How many CC's can come of 1 spore print? [Re: MagicMaker]
#10897031 - 08/20/09 11:50 AM (14 years, 7 months ago) |
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Quote:
MagicMaker said: roger rabbit i know this is a super old post but wanted to help your math out and let you know that a 4in diam cap is more then 2x a 2in diam cap http://www.mathgoodies.com/lessons/vol2/circle_area.html
your being really tedious about this.....it doesnt have to be on the dot..but the fact is...he's right ...it depends on the size of of the print....but an average side print can yeild a estimate of 3 syringes ,just off the bat..but its a personal preference to keep my syringes dark and heavy with spores......its possible to max out that print 2x... and have a visiblt clear viable syringe...
-------------------- Please +REP if i've been of any help... thanks!!! Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol
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