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Offlinetibberous
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Registered: 12/26/04
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Brain Heart Infusion Agar?
    #7022765 - 06/08/07 12:54 PM (16 years, 10 months ago)

I was searching for agar online and found some called Brain Heart Infusion Agar, with %5 sheep's blood. Unlike other types of agar, this one looks like it only supports fungi growth:

https://www.hardydiagnostics.com/catalog2/user/ProductDetails.asp?poid=W185BX

That site has a ton of different agar mixes, some that come with antibiotics mixed in to help fight contaims. Would these be better than normal MEA and PDA agar?

They also carry Sabouraud Agar.

Sabouraud Agar : Used for fungi and has a low pH that will kill most bacteria. It contains gentamicin, which is a aminoglycoside antibiotic. Gentamicin can also treat many different types of bacterial infections, particularly Gram-negative infection.

Edited by tibberous (06/08/07 12:57 PM)

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InvisibleBrainiac
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Re: Brain Heart Infusion Agar? [Re: tibberous]
    #7022812 - 06/08/07 01:09 PM (16 years, 10 months ago)

No, because they can grow other kinds of fungi. That you really not won't.I would go with the pda.The only time that you really should use a antibiotic agar.Is if you're cleaning up wild spores.For a much cheaper way, try using cardboard.


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:Awesketch:

:cool: Fair is Fair :devil:

Edited by Brainiac (06/08/07 01:45 PM)

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OfflineMYSTIQUE
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Re: Brain Heart Infusion Agar? [Re: Brainiac]
    #7022894 - 06/08/07 01:30 PM (16 years, 10 months ago)

You are just looking for troubles with the blood agar its made for growing viruses and bacteria not molds and mysc and you will end up with some super virus on there.


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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

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Offlinemakaveli8x8
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Re: Brain Heart Infusion Agar? [Re: MYSTIQUE]
    #7022982 - 06/08/07 02:01 PM (16 years, 10 months ago)

you know a long time ago i had a post about a theory of mine, something along the lines of mushrooms contain a drug so that when an animal eats it, it gets confused and is more likely to die near the area of the mushroom

hence leading to the mushroom below the soil eventually eating material left from this animal....at the very least the animal is going to fertilize the soil.

anyways someone said mushrooms don't eat animals and now at least I know they eat blood :crazy2:


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We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.

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InvisibleBrainiac
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Re: Brain Heart Infusion Agar? [Re: makaveli8x8]
    #7022990 - 06/08/07 02:04 PM (16 years, 10 months ago)

Some mushrooms eat bugs.


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:Awesketch:

:cool: Fair is Fair :devil:

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Offlinemakaveli8x8
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Re: Brain Heart Infusion Agar? [Re: Brainiac]
    #7022998 - 06/08/07 02:06 PM (16 years, 10 months ago)

yep the spores germinate inside there body and then it fruits threw their chest like an alien....wicked stuff.


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.

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Offlinemakaveli8x8
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Re: Brain Heart Infusion Agar? [Re: makaveli8x8]
    #7023002 - 06/08/07 02:07 PM (16 years, 10 months ago)

ive actually thought about getting some of them spores but then get freaked out thinking it could mutate into a human strain....just immagine....


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.

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OfflineMYSTIQUE
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Re: Brain Heart Infusion Agar? [Re: makaveli8x8]
    #7023008 - 06/08/07 02:08 PM (16 years, 10 months ago)

There is a sweet video on youtube on a mushroom killing then growing out of some kind of ant I think.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

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OfflineMYSTIQUE
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Re: Brain Heart Infusion Agar? [Re: MYSTIQUE]
    #7023011 - 06/08/07 02:10 PM (16 years, 10 months ago)



--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

Extras: Filter Print Post Top
InvisibleBrainiac
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Registered: 04/29/06
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Re: Brain Heart Infusion Agar? [Re: MYSTIQUE]
    #7023022 - 06/08/07 02:13 PM (16 years, 10 months ago)

I thank Hypsizygus tessulatus "Buna-Shimeji", "The Beech Oyster" eats bugs.


--------------------
:Awesketch:

:cool: Fair is Fair :devil:

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Offlinemakaveli8x8
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Re: Brain Heart Infusion Agar? [Re: MYSTIQUE]
    #7023023 - 06/08/07 02:13 PM (16 years, 10 months ago)

no more no more!


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.

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Offlinemakaveli8x8
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Re: Brain Heart Infusion Agar? [Re: makaveli8x8]
    #7023029 - 06/08/07 02:15 PM (16 years, 10 months ago)

like in the video?


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.

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InvisibleBlutjager
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Registered: 06/11/06
Posts: 9,220
Re: Brain Heart Infusion Agar? [Re: makaveli8x8]
    #7023324 - 06/08/07 03:57 PM (16 years, 10 months ago)

I have seen pics of that before but never a video,thanks :yesnod:

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OfflineMYSTIQUE
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Re: Brain Heart Infusion Agar? [Re: Blutjager]
    #7023358 - 06/08/07 04:07 PM (16 years, 10 months ago)



--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

Extras: Filter Print Post Top
InvisibleBrainiac
Rogue Scientist
Male


Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋 Flag
Re: Brain Heart Infusion Agar? [Re: tibberous]
    #7023972 - 06/08/07 07:36 PM (16 years, 10 months ago)

They might not even sell it to you.


--------------------
:Awesketch:

:cool: Fair is Fair :devil:

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OfflineCaptainLinger
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Registered: 05/25/07
Posts: 1,756
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Re: Brain Heart Infusion Agar? [Re: Brainiac]
    #7024184 - 06/08/07 08:27 PM (16 years, 10 months ago)

makaveli8x8...actually your statement reminds me of some reading I did in college a few years back about the cult of the Fly Agaric. Reindeer in Siberia actually *did* harbor a severe, and often fatal, love for mushrooms. They'd eat to their heart's content, and effectively pass out on the couch for a few days. This left them vulnerable to the humans around, who just walked up, and slit their throats (they get bonus points for the foresight to attach urine collection bags to the animals, thereby getting the agaric actives, without toxins).

There has been some speculation as to whether this would yield psychedelic steak or not :wink:

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InvisibleAlkaloids
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Re: Brain Heart Infusion Agar? [Re: tibberous]
    #7027001 - 06/09/07 04:10 PM (16 years, 10 months ago)

That agar is most likely way too expensive and wouldn't be suitable for the type of work you are doing.  Most agar that is sold from lab supply stores is optimized for the "lower" fungi like molds and mildews which tend to prefer most acidic environments.  Someone please correct me if i'm wrong on that.

  Anti-biotic agar can be useful for some isolation work, but if you are just starting off the remember the KISS method.  Keep It Stupid Simple  :smile:


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Offlinealbo
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Registered: 01/24/10
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Re: Brain Heart Infusion Agar? [Re: Alkaloids]
    #11889120 - 01/24/10 03:47 AM (14 years, 2 months ago)

hi there, sorry I'm a bit new to all this forum stuff... i have access to lots of lab equipment and would just like to say that spores grow fine in brain heart media, and it is a perfect way to store in the fridge. Also if you can get hold of blood culture bottles (aerobic, pediatric and mycology ones all work perfectly, and come complete with sterile injector sites on them.......

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