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TheCheshireCat
Stranger
Registered: 05/28/07
Posts: 3
Last seen: 16 years, 9 months
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Mycelium not growing, NOTHING growing
#6977294 - 05/28/07 11:04 AM (16 years, 9 months ago) |
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I have a jar that I inoculated well over a month ago following PF tek exactly. However, there has been no myseclium growth whatsoever. In fact, there has been no growth whatsoever. The only thing I was thinking could have gone wrong is that after I flame-sterilized my needle I put it in the jar too quick and the hot metal killed the spores before they got in. Anyone know what went wrong? Is it possible to reinoculate it and save it, or do I need new substrate altogether.
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xaxphaanes
Mycologist
Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 7 months
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Re: Mycelium not growing, NOTHING growing [Re: TheCheshireCat]
#6977307 - 05/28/07 11:08 AM (16 years, 9 months ago) |
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bunk syringe ?
-------------------- "Anything i say is fictional" what you should look for in manure
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TheCheshireCat
Stranger
Registered: 05/28/07
Posts: 3
Last seen: 16 years, 9 months
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Re: Mycelium not growing, NOTHING growing [Re: xaxphaanes]
#6977323 - 05/28/07 11:12 AM (16 years, 9 months ago) |
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I ordered it from The Hawk's Eye
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biggysmall
Stranger
Registered: 04/27/07
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Loc: your moms bedroom
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Re: Mycelium not growing, NOTHING growing [Re: TheCheshireCat]
#6977354 - 05/28/07 11:17 AM (16 years, 9 months ago) |
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ya but it doesn't mean it is always guaranteed..
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Mankey
Registered: 07/30/05
Posts: 2,203
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Re: Mycelium not growing, NOTHING growing *DELETED* [Re: TheCheshireCat]
#6977359 - 05/28/07 11:18 AM (16 years, 9 months ago) |
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Post deleted by MankeyReason for deletion: .
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TheCheshireCat
Stranger
Registered: 05/28/07
Posts: 3
Last seen: 16 years, 9 months
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Re: Mycelium not growing, NOTHING growing [Re: Mankey]
#6977383 - 05/28/07 11:23 AM (16 years, 9 months ago) |
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I waited until the pressure cooker was cool to the touch and the jar was too, many hours.
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Handyathome
Stranger
Registered: 05/28/07
Posts: 4
Loc: Way out
Last seen: 16 years, 9 months
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Re: Mycelium not growing, NOTHING growing [Re: TheCheshireCat]
#6977591 - 05/28/07 12:11 PM (16 years, 9 months ago) |
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I am just a noob too. I inoculated my first set of 7 jars three days ago. There are in an incubator at 80F.....So how long does it take to see something?
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Mankey
Registered: 07/30/05
Posts: 2,203
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Re: Mycelium not growing, NOTHING growing *DELETED* [Re: Handyathome]
#6977600 - 05/28/07 12:13 PM (16 years, 9 months ago) |
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Post deleted by MankeyReason for deletion: .
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myndreach
philosopher
Registered: 08/07/04
Posts: 2,368
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Re: Mycelium not growing, NOTHING growing [Re: Mankey]
#6977611 - 05/28/07 12:16 PM (16 years, 9 months ago) |
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PM the vendor, I'm sure they will make it right.
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Mankey
Registered: 07/30/05
Posts: 2,203
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Re: Mycelium not growing, NOTHING growing *DELETED* [Re: myndreach]
#6977620 - 05/28/07 12:19 PM (16 years, 9 months ago) |
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Post deleted by MankeyReason for deletion: .
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
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Re: Mycelium not growing, NOTHING growing [Re: Mankey]
#6977962 - 05/28/07 02:00 PM (16 years, 9 months ago) |
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What about the other jars? Was there only one that showed no growth? A 10ml syringe will inoculate at least 10 pf jars, so how did the other nine fare?
I always flame the needle, and then almost immediately inject. This ensures the needle is clean and contaminant free. The first drop of solution might sizzle a bit, but then the rest can flow unimpeded and sterile. Flame between each jar, but not between holes in the same jar. This prevents cross-contamination should the sterilization process not have been complete in one or two jars.
Growth is usually noticable within a week. Many experienced growers have their jars fully colonized after two to three weeks. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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I Like Alkaloids
Brought Back to Life
Registered: 05/14/07
Posts: 516
Loc: Rocky Mountains, U.S.
Last seen: 8 years, 1 month
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Re: Mycelium not growing, NOTHING growing [Re: TheCheshireCat]
#6978007 - 05/28/07 02:17 PM (16 years, 9 months ago) |
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Quote:
TheCheshireCat said: I have a jar that I inoculated well over a month ago following PF tek exactly.
Did you only inoculate one jar? How much spore solution did you use? Keep your jar lids loose during PC and incubation, and remove the foil while incubating. The dry vermiculite should stop airborne contams from entering the jar.
I had similar problems a long time ago, so I went to Ralph. Jars colonize in about 2 weeks!
I'm no pro, but this worked for me. Good Luck
-------------------- A lot of people here are assholes, but so am I...
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Handyathome
Stranger
Registered: 05/28/07
Posts: 4
Loc: Way out
Last seen: 16 years, 9 months
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Re: Mycelium not growing, NOTHING growing [Re: TheCheshireCat]
#6980554 - 05/29/07 12:53 AM (16 years, 9 months ago) |
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I made up my own syringe....I know...a little bit of a stretch for a noob, but i did work in a glove box with everything pressure cooked bar the spore print. I was very careful and methodical - worked everything out in my head before i started. Ok so still to early to tell! will let you know.
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Glasskannon
I play WoW!! lol
Registered: 05/26/07
Posts: 68
Last seen: 10 years, 8 months
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Re: Mycelium not growing, NOTHING growing [Re: Handyathome]
#6980619 - 05/29/07 01:28 AM (16 years, 9 months ago) |
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Well you may have answered your own question :P
Im pretty new to this as well but i have read a LOT of stuff on the shroomery and have learned a lot...
I know i wont be attempting to make my own syringes for my first grow.
Im actually waiting for mine to arrive in the mail
EDIT: btw im not trying to sound like an ass or anything to you im just saying that me personally... i want to have a successfully grow before i dive into the world of making my own syringes.
Edited by Glasskannon (05/29/07 01:31 AM)
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Nibin
Getting there
Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 10 months
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Re: Mycelium not growing, NOTHING growing [Re: Glasskannon]
#6980728 - 05/29/07 03:12 AM (16 years, 9 months ago) |
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Original poster DID buy a syringe of a vendor. It's the second guy who made his own.
-------------------- Newcomers guide-----> For all things shroomy
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