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 PitcherCrab
Crescent Fresh
 
Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 1 year, 4 months
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 pasteurizing straw 
#6902731 - 05/11/07 04:02 PM (16 years, 11 months ago) |
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From everything I've read I've come to the understanding that most people use hydrated lime and bleach when pasteurizing straw, but I just talked to a guy that grows bulk oysters who doesn't use anything. He doesn't like to use it because he wants his product to be completely organic and he said that it slows down the colonization time of the mycelium. Has anyone here pasteurized without the chemicals? If so how long did you heat it and did you have any problems with contamination?
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PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog
“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London 
Last seen: 7 years, 3 months
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Re: pasteurizing straw [Re: PitcherCrab]
#6902769 - 05/11/07 04:10 PM (16 years, 11 months ago) |
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never used chemicals, just heated straw in a pillow case in a big pot on the stove for 2 hours at 160-170, drained over the sink. had a few contams from time to time, sure, but i've been pretty happy with this low-tek
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buh
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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I've never used bleach, but I do use lime, which is mineral and not a chemical. The lime is not for pasteurization, but rather to leave the straw with a high pH afterward, so it can colonize without being invaded by trichoderma which loves a low pH. I use it in the pasteurization bath as a matter of convience, rather than to help pasteurize the straw.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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PitcherCrab
Crescent Fresh
Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 1 year, 4 months
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Re: pasteurizing straw [Re: RogerRabbit]
#6903193 - 05/11/07 06:07 PM (16 years, 11 months ago) |
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Ah... if I don't use lime do you think I'll run into trouble?
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PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog
“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: pasteurizing straw [Re: PitcherCrab]
#6903532 - 05/11/07 07:36 PM (16 years, 11 months ago) |
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You're more likely to. I get pinning on oysters within 9 to 11 days after inoculation, so your friend is dead wrong about it slowing down colonization. The lime just helps to prevent the trichoderma spores from germinating before the mushroom mycelium colonizes the entire substrate. Since it's hard to build a straw project in complete sterility, you should assume that contaminant spores are going to be on it.
RR
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
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Re: pasteurizing straw [Re: RogerRabbit]
#6903670 - 05/11/07 08:08 PM (16 years, 11 months ago) |
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I dont use anything but bleach, I pasturize the straw for at least 2 hours at above 160 and below 180.
So far, no problems.
-Graham
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www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
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orders@mycopath.com
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PitcherCrab
Crescent Fresh
Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 1 year, 4 months
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Re: pasteurizing straw [Re: Cryogenicz]
#6904195 - 05/11/07 09:49 PM (16 years, 11 months ago) |
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Well... I am pasteurizing the second batch of straw in a 5 gallon pot as I type this. The first batch just got down to about 80-85, so after I write this I am going to go inoculate it. I didn't put in bleach or lime, so I'm gonna keep my fingers crossed that they won't get contaminated... I am using two quarts of spawn in milk crates.
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PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog
“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
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Re: pasteurizing straw [Re: PitcherCrab]
#6904518 - 05/11/07 11:32 PM (16 years, 11 months ago) |
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Have fun! 
-Graham
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www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.
www.FungiForum.com
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 3 months
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Re: pasteurizing straw [Re: PitcherCrab]
#6906559 - 05/12/07 04:47 PM (16 years, 11 months ago) |
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Quote:
PitcherCrab said: The first batch just got down to about 80-85
pls explain
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buh
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
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The straw he pasteurized has cooled down to 80-85F, so its about ready to inoculated.
-Graham
--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.
www.FungiForum.com
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 zee_werp
a fractalcreature
Registered: 03/24/03
Posts: 1,026
Loc: Aotearoa
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Most of the time I just pasturise with plain heat. Bales of straw submerged in 65 degree C water for 2 hours. I get maybe 1 in 100 bags contamed. Oysters are pretty damn resilient, they colonise it so fast if you use a high enough spawn rate.
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
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Re: pasteurizing straw [Re: zee_werp]
#6906744 - 05/12/07 06:07 PM (16 years, 11 months ago) |
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What container do you submerge the entire bales in?
-Graham
--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.
www.FungiForum.com
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zee_werp
a fractalcreature
Registered: 03/24/03
Posts: 1,026
Loc: Aotearoa
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Re: pasteurizing straw [Re: Cryogenicz]
#6907777 - 05/12/07 10:30 PM (16 years, 11 months ago) |
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I've got a nice rectangular stainless steel tank that fits 4 bales. It's pretty good for doing bulk amounts. Takes 8 hours to heat up with a BBQ under it and a 2kw submersion heater.
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
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Re: pasteurizing straw [Re: zee_werp]
#6907842 - 05/12/07 10:45 PM (16 years, 11 months ago) |
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Where did you get it?
Can you get some pictures of it?
-Graham
--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.
www.FungiForum.com
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zee_werp
a fractalcreature
Registered: 03/24/03
Posts: 1,026
Loc: Aotearoa
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Re: pasteurizing straw [Re: Cryogenicz]
#6908040 - 05/12/07 11:39 PM (16 years, 11 months ago) |
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I got it from a seconhand industrial scrap parts dealer. It was pretty sweet cause I picked it up for the price of scrap (by weight). Might get some pics of it and the rest of my setup sometime soon.
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
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Re: pasteurizing straw [Re: zee_werp]
#6909491 - 05/13/07 10:59 AM (16 years, 11 months ago) |
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nice, how much was it?
-Graham
--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.
www.FungiForum.com
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