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S1lverdj
Stranger
Registered: 07/22/06
Posts: 179
Last seen: 5 years, 9 months
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Rye Jars Growing Way Too Slow
#6734202 - 04/01/07 08:26 AM (17 years, 1 day ago) |
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I inoculated these quart jars back on 03/07/07 with a newly made LC. It is now 04/01/07 and 3 of the jars are showing minimal growth and 2 of the jars are showing no growth whatsoever. One jar was lost to green mold (I know what happened here). The jars are being kept in a TiT at around 80 degrees. They were each inoculated with 4cc's of very healthy LC. Anyone have any ideas?
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doc34
Fungitarian
Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
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Re: Rye Jars Growing Way Too Slow [Re: S1lverdj]
#6734270 - 04/01/07 08:55 AM (17 years, 1 day ago) |
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Quote:
I inoculated these quart jars back on 03/07/07 with a newly made LC. It is now 04/01/07 and 3 of the jars are showing minimal growth and 2 of the jars are showing no growth whatsoever
Either your jars are too wet, or your lc is bad. I don't think they are too dry considering youQuote:
inoculated with 4cc's of very healthy LC
. If you inoculated with a viable lc you should have colonized jars within 6-10 days,depending. My jars colonize in 6 days(qt's)with my Lc's.
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EntheogenicPeace
Scholar
Registered: 10/04/05
Posts: 3,926
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Re: Rye Jars Growing Way Too Slow [Re: S1lverdj]
#6734286 - 04/01/07 09:03 AM (17 years, 1 day ago) |
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Edited by EntheogenicPeace (12/25/14 06:09 PM)
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EntheogenicPeace
Scholar
Registered: 10/04/05
Posts: 3,926
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Re: Rye Jars Growing Way Too Slow [Re: S1lverdj]
#6734292 - 04/01/07 09:06 AM (17 years, 1 day ago) |
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Edited by EntheogenicPeace (12/25/14 06:10 PM)
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S1lverdj
Stranger
Registered: 07/22/06
Posts: 179
Last seen: 5 years, 9 months
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Positive it's mycelium. I believe the jars may just be too wet as there is quite a bit standing in the bottom.
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Rye Jars Growing Way Too Slow [Re: S1lverdj]
#6735514 - 04/01/07 05:09 PM (17 years, 17 hours ago) |
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Quote:
S1lverdj said: Positive it's mycelium. I believe the jars may just be too wet as there is quite a bit standing in the bottom.
If you have standing/pooling water in the bottom of a wbs/grain jar. I suggest you START OVER. As the jars will fubar/contam/stall sooner or later.
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,981
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Re: Rye Jars Growing Way Too Slow [Re: agar]
#6736417 - 04/01/07 09:41 PM (17 years, 12 hours ago) |
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Water in the bottom is a sure recipe for failure from bacterial contams.
So you either added too much water, or it leaked in during sterilization. (the answer would depend on how you prepared the jars)
Whats in those jars by the way? WBS? Rye?
Water content of a wbs, rye, or pf jar is sooo important, you gotta get that part right.
If you don't see any growth in a jar in the first 2 weeks, (1 week for LC, you should see it colonizing in the first day or so) something is wrong, and from my exp its almost always bacterial.
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iSlak
Stranger
Registered: 11/28/06
Posts: 59
Last seen: 15 years, 11 months
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Re: Rye Jars Growing Way Too Slow [Re: simplemachine]
#6737121 - 04/02/07 03:44 AM (17 years, 6 hours ago) |
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Quote:
simplemachine said: Water content of a wbs, rye, or pf jar is sooo important, you gotta get that part right.
How do I get the water content right? I'm having issues with jars colonizing slowly and not so well. I suspect moisture issues.
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deucedbi9
Stranger
Registered: 10/24/06
Posts: 4,613
Loc: UK
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Re: Rye Jars Growing Way Too Slow [Re: iSlak]
#6737141 - 04/02/07 04:25 AM (17 years, 6 hours ago) |
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after cooking the grain make sure to rinse (with hot water)and strain well. better yet,dump the grain into a container (i use a food mixing bowl for small amounts) and blow dry with a hair drier. when all the grains no longer feels wet or sticks to your hand, or a loaded spoon when "upended" load your jars. make sure the jars are dry before loading.
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
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iSlak
Stranger
Registered: 11/28/06
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Last seen: 15 years, 11 months
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Re: Rye Jars Growing Way Too Slow [Re: deucedbi9]
#6738432 - 04/02/07 02:11 PM (16 years, 11 months ago) |
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What I usually do is soak the grain for 12-24 hours. Then I dump it into a strainer and give it a thorough washing. After which I'll let the grain sit in the strainer for a couple of minutes or immediately start loading the grain into jars.
I never cook my grains beforehand. Also, I'm not so sure about drying the grains so much before PC'ing. My jars seem quite high and dry as it is.
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,981
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Re: Rye Jars Growing Way Too Slow [Re: iSlak]
#6738508 - 04/02/07 02:34 PM (16 years, 11 months ago) |
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Quote:
iSlak said:
Quote:
simplemachine said: Water content of a wbs, rye, or pf jar is sooo important, you gotta get that part right.
How do I get the water content right? I'm having issues with jars colonizing slowly and not so well. I suspect moisture issues.
Well that depends on what you're doing wrong!
Grains don't have to be blow dried, some ppl do it, but they just need to be drained right. After soaking you have to drain the grain till no water drips off the bottom of the colander. The other possibility is water leaking into the jar during PCing. If thats the problem, just use less water in the PC! You only need a few inches in the bottom, thats it.
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,981
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Re: Rye Jars Growing Way Too Slow [Re: iSlak]
#6738516 - 04/02/07 02:37 PM (16 years, 11 months ago) |
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Quote:
iSlak said: What I usually do is soak the grain for 12-24 hours. Then I dump it into a strainer and give it a thorough washing. After which I'll let the grain sit in the strainer for a couple of minutes or immediately start loading the grain into jars.
Ahhhh...there's the problem! gotta let them puppies drain for 30-60 minutes...Shake the strainer up and down...When no more water drips off, then you load and PC.
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iSlak
Stranger
Registered: 11/28/06
Posts: 59
Last seen: 15 years, 11 months
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Re: Rye Jars Growing Way Too Slow [Re: simplemachine]
#6740642 - 04/02/07 10:53 PM (16 years, 11 months ago) |
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Quote:
simplemachine said: Ahhhh...there's the problem! gotta let them puppies drain for 30-60 minutes...Shake the strainer up and down...When no more water drips off, then you load and PC.
Righteous!
Does this also apply to WBS?
Edited by iSlak (04/02/07 10:54 PM)
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EntheogenicPeace
Scholar
Registered: 10/04/05
Posts: 3,926
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Re: Rye Jars Growing Way Too Slow [Re: iSlak]
#6741812 - 04/03/07 11:24 AM (16 years, 11 months ago) |
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Edited by EntheogenicPeace (12/25/14 06:10 PM)
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JonnyOneNut
Sponge
Registered: 11/13/06
Posts: 303
Loc: Massachusetts
Last seen: 8 years, 1 month
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-------------------- BABY BOTTLE LC TEK Fix it or accept it, but do not stress about it.
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Cyric
Master of Time and Space
Registered: 07/12/06
Posts: 340
Loc: Outer Shpongolia
Last seen: 14 years, 4 months
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Re: Rye Jars Growing Way Too Slow [Re: JonnyOneNut]
#6742309 - 04/03/07 01:29 PM (16 years, 11 months ago) |
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Titmouse and tarnation, now I know why I'm not getting very good results.... Shit, and all this time I thought it was because of LACK of moisture.
Oh well, my new syringe came yesterday, this time I'll get it right!
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EntheogenicPeace
Scholar
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Posts: 3,926
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Re: Rye Jars Growing Way Too Slow [Re: Cyric]
#6742361 - 04/03/07 01:41 PM (16 years, 11 months ago) |
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Edited by EntheogenicPeace (12/25/14 06:10 PM)
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Cyric
Master of Time and Space
Registered: 07/12/06
Posts: 340
Loc: Outer Shpongolia
Last seen: 14 years, 4 months
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I just feel stupid now - So if there's ANY extra humidity in there, it's a bad thing? I've got beads of water here and there. I was always afraid of PCing for too long, and the kernels look like they're kind of cooked, you know?
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EntheogenicPeace
Scholar
Registered: 10/04/05
Posts: 3,926
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Re: Rye Jars Growing Way Too Slow [Re: Cyric]
#6742909 - 04/03/07 04:13 PM (16 years, 11 months ago) |
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Edited by EntheogenicPeace (12/25/14 06:10 PM)
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EntheogenicPeace
Scholar
Registered: 10/04/05
Posts: 3,926
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Re: Rye Jars Growing Way Too Slow [Re: Cyric]
#6742921 - 04/03/07 04:18 PM (16 years, 11 months ago) |
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Edited by EntheogenicPeace (12/25/14 06:10 PM)
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