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Ogla
Registered: 02/16/04
Posts: 11,325
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liquid Mycelium Syringe question
#6635328 - 03/04/07 06:59 PM (17 years, 19 days ago) |
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according to Uncle Lazlo's EZ Mycelium Syringe Tek (http://www.shroomery.org/65/Uncle-Lazlos-EZ-Mycelium-Syringe-Tek)
you take a jar of water, sterilize it, put a piece of colonized cake in the steril jar of water, shake and suck into syringe..
would it be more proper to instead use a honey/water mixture with a piece of colonized cake? if so, should i wait for it to grow inside the jar or suck it in the syringe and let it grow in the syringe? Same basic concept, right?
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KaptKid
Spaced Pirate
Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 1 month
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Re: liquid Mycelium Syringe question [Re: Ogla]
#6635465 - 03/04/07 07:35 PM (17 years, 18 days ago) |
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I was wondering how this thread is being look at by so many, so fast ?????????
-------------------- Child of the 60's, Tripping ever since.
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LayYouIn
Taurus
Registered: 09/28/06
Posts: 4,402
Loc: Organ
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Re: liquid Mycelium Syringe question [Re: KaptKid]
#6635466 - 03/04/07 07:36 PM (17 years, 18 days ago) |
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Quote:
KaptKid said: I was wondering how this thread is being look at by so many, so fast ?????????
freddy must have some fans.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 29 days
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Re: liquid Mycelium Syringe question [Re: LayYouIn]
#6635471 - 03/04/07 07:38 PM (17 years, 18 days ago) |
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You don't want nutrients in a mycelium syringe. They're not for growing mycelium, which would clog the syringe. They're for inoculation. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Optx
PronouncedAwp-Tiks
Registered: 03/28/05
Posts: 977
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Re: liquid Mycelium Syringe question [Re: Ogla]
#6635497 - 03/04/07 07:45 PM (17 years, 18 days ago) |
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use honey water or karo, sterilize, add healthy mycellium or spores, let grow, suck up lots more healthy mycellium into syringe
-------------------- please do not take everything i say here so personally. welcome to teh internets!
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Ogla
Registered: 02/16/04
Posts: 11,325
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Re: liquid Mycelium Syringe question [Re: Optx]
#6635699 - 03/04/07 08:44 PM (17 years, 18 days ago) |
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yeah thats wierd, how the hell did i get so many view so quick?? But thanks Optx. seems pretty basic. Reason i ask is because i have no spores, only one healthy colonized cake that i need to clone. But thanks everyone for your input.
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Ogla
Registered: 02/16/04
Posts: 11,325
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Re: liquid Mycelium Syringe question [Re: Ogla]
#6635706 - 03/04/07 08:48 PM (17 years, 18 days ago) |
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oh, is it ok if it is karo with vanilla? all the corn syrup on the shelf seem to have vanilla in it.
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Optx
PronouncedAwp-Tiks
Registered: 03/28/05
Posts: 977
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Re: liquid Mycelium Syringe question [Re: Ogla]
#6635800 - 03/04/07 09:17 PM (17 years, 18 days ago) |
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i couldn't tell ya for sure, i've only done honey water LCs. vanilla has sugars in it though so i don't see why it wouldn't work, just don't put too much
-------------------- please do not take everything i say here so personally. welcome to teh internets!
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid Mycelium Syringe question [Re: Ogla]
#6635804 - 03/04/07 09:18 PM (17 years, 18 days ago) |
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Water might be sterile. A CAKE is not. It will turn NASTY
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Optx
PronouncedAwp-Tiks
Registered: 03/28/05
Posts: 977
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Re: liquid Mycelium Syringe question [Re: agar]
#6635813 - 03/04/07 09:20 PM (17 years, 18 days ago) |
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ok, it might not be sterile, but wouldn't using a bit of cake/myc be like squirting water into a colonized grain jar and shaking, then sucking up the myc-water?
-------------------- please do not take everything i say here so personally. welcome to teh internets!
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Ogla
Registered: 02/16/04
Posts: 11,325
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Re: liquid Mycelium Syringe question [Re: Optx]
#6635951 - 03/04/07 10:08 PM (17 years, 18 days ago) |
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well the original tek in the link above said to use only water., but other similar teks use a water/honey(karo) mixture. I really dont see what the differnce would be, cept that the honey(karo) would allow the myc to grow in the liquid., as opposed to just being disperssed in plain water.
Quote:
agar said: Water might be sterile. A CAKE is not. It will turn NASTY
but a fully colonized cake would resist contamination/ turn nasty.
Edited by Ogla (03/04/07 10:10 PM)
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Optx
PronouncedAwp-Tiks
Registered: 03/28/05
Posts: 977
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Re: liquid Mycelium Syringe question [Re: Ogla]
#6636023 - 03/04/07 10:27 PM (17 years, 18 days ago) |
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well, i guess it all depends on the application of this mycellium syringe. if it's for storage then yeah, just water and myc. if you're planning on taking a little myc and turning that into lots, then using it to inoculate jars right away - then the karo or honeywater route would be used
-------------------- please do not take everything i say here so personally. welcome to teh internets!
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