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OfflineS1lverdj
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WBS REFUSING to colonize 100%
    #6479088 - 01/20/07 03:14 AM (17 years, 2 months ago)

My WBS jars have been at like 98% colonization for days now. Should I shake them to redistribute the grain, or what? They've been in the incubator with my rye jars and the rye jars are doing great.

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Invisibleagar
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Re: WBS REFUSING to colonize 100% [Re: S1lverdj]
    #6479163 - 01/20/07 04:44 AM (17 years, 2 months ago)

Have patience.
To much shaking.
Can reverse progress.


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OfflineS1lverdj
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Re: WBS REFUSING to colonize 100% [Re: agar]
    #6479205 - 01/20/07 05:56 AM (17 years, 2 months ago)

Thanks, agar. I've never been very good at patience.

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Offlinetonyperez420
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Re: WBS REFUSING to colonize 100% [Re: S1lverdj]
    #6479207 - 01/20/07 05:59 AM (17 years, 2 months ago)

fuck patience, it usually never gets anything done

you never want to shake a jar if its over 50% colonized
I do it at about 25-30 %

BUT 98% should be enough colonization to move on to the next phase lol its prob ready

Edited by tonyperez420 (01/20/07 06:00 AM)

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InvisibleSillicybin
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Re: WBS REFUSING to colonize 100% [Re: tonyperez420]
    #6479484 - 01/20/07 10:25 AM (17 years, 2 months ago)

I've had jars stall with very little left to do also... as long as it's not obviously infected with bacteria, I'll just pop the colonized mycelium out and separate out and throw away the uncolonized portion.

EDIT: The reason my jars stalled was because some of the grains were just way too dry.

Edited by Sillicybin (01/20/07 11:21 AM)

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OfflineRogerRabbitM
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Re: WBS REFUSING to colonize 100% [Re: Sillicybin]
    #6479500 - 01/20/07 10:36 AM (17 years, 2 months ago)

They don't 'stall' without a reason. Either it ran out of air due to no holes for gas exchange, or it's contaminated with bacteria. You can't spawn uncolonized grains or the bulk is sure to contaminate.

If you're using quart/liter jars, they shouldn't be in an incubator. High temps stimulate bacteria, so let them colonize at normal room temperature. (72-74) Anything 80F or less will be fine.
RR


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Offline2FiNiTe
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Re: WBS REFUSING to colonize 100% [Re: tonyperez420]
    #6479942 - 01/20/07 01:40 PM (17 years, 2 months ago)

:piss:
Quote:

tonyperez420 said:
fuck patience, it usually never gets anything done





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"Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living."

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InvisibleBlutjager
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Re: WBS REFUSING to colonize 100% [Re: Sillicybin]
    #6480067 - 01/20/07 02:37 PM (17 years, 2 months ago)

Quote:

Sillicybin said:
I've had jars stall with very little left to do also... as long as it's not obviously infected with bacteria, I'll just pop the colonized mycelium out and separate out and throw away the uncolonized portion.

EDIT: The reason my jars stalled was because some of the grains were just way too dry.




I could have made the exact same post except for the opposite reason,thats why I hardly use birdseed anymore,I have tried like 4 different wbs teks and no matter what there is always a good half inch at the bottom that is too wet to colonize,Just use the colonized portion and make sure not to use the seeds that are not covered in mycelium

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Offline2FiNiTe
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Re: WBS REFUSING to colonize 100% [Re: Blutjager]
    #6480232 - 01/20/07 04:12 PM (17 years, 2 months ago)

I have the exact same problem man I really just don't know what I was doing wrong, each consecutive time I tried to use less moisture content, tried my hardest to get it even below field capacity. None the less I had the same result every single time. The bottom of the jar filled with fluid unable to colonize and eventually starting contam. of some kind.


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"Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living."

General Omar N. Bradley

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InvisibleBlutjager
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Re: WBS REFUSING to colonize 100% [Re: 2FiNiTe]
    #6480300 - 01/20/07 04:38 PM (17 years, 2 months ago)

Yea,I cant figure it out,I have used longer soaks and shorter soaks,longer simmer and shorter simmer,every time its the same thing,I'm just not meant to use wbs I guess :lol:

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OfflineS1lverdj
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Re: WBS REFUSING to colonize 100% [Re: 2FiNiTe]
    #6480301 - 01/20/07 04:38 PM (17 years, 2 months ago)

What happens if you do shake after 50%? Just curious as I had been considering this and was wondering what disaster could've happened.

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InvisibleBlutjager
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Re: WBS REFUSING to colonize 100% [Re: S1lverdj]
    #6480313 - 01/20/07 04:43 PM (17 years, 2 months ago)

It doesn't matter no matter how hard you shake...even if you bang the jar against something it wont break up,its a solid mass of mushy crap at the bottom of the jar

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Invisibletakk
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Re: WBS REFUSING to colonize 100% [Re: Blutjager]
    #6480597 - 01/20/07 06:39 PM (17 years, 2 months ago)

sounds like bacterial endospores having fun with your wbs

maybe

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InvisibleBlutjager
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Re: WBS REFUSING to colonize 100% [Re: takk]
    #6480780 - 01/20/07 07:43 PM (17 years, 2 months ago)

Quote:

Anarchyz0r said:
sounds like bacterial endospores having fun with your wbs

maybe




Nope,just a bunch of busted starchy mush that the mycelium cant get through,the colonized parts have always spawned to my substrate just fine so I don't think its bacteria,more of an issue of me making some mistake along the line during preparation

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OfflineLun4e
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Re: WBS REFUSING to colonize 100% [Re: Blutjager]
    #6481502 - 01/21/07 01:09 AM (17 years, 1 month ago)

You could try no simmer. The jars AFOAF had success with had minimall prepared WBS, which was only rinsed and floaters removed. Then it went in jars with water, so the water line was 2/3 as high as the WBS. Cooked for 1:30 at 15PSI. Don't see a single busted kernel, and the water content is perfect. Good luck!


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How to make your 50/50 casing 50/50+ with only eggshells:
http://www.shroomery.org/forums/showflat.php?Cat=0&Number=6519162&page=0&vc=#Post6519162

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InvisibleBlutjager
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Re: WBS REFUSING to colonize 100% [Re: Lun4e]
    #6481538 - 01/21/07 01:32 AM (17 years, 1 month ago)

Maybe I will try no simmer with wbs when I run out of rye,I would love to get good at birdseed considering its like a third of the price I pay for my rye and I can get it at the pet store,I have to special order my rye and wait for it to come in...its a pain but it beats jars full of birdseed that wont colonize,Ill get it right one of these days:thumbup:

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