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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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jellyfish
Registered: 10/02/05
Posts: 7,457
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Simple glove box
#6463608 - 01/15/07 07:22 PM (17 years, 2 months ago) |
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I bought a see through rubber maid container with a hinged lid that does not seal at all around the edges. I plan on cuttign two arm holes and connectting garbage bags to the holes with duct tape and attackthe gloves to the bags. And seal the top with duct tape each time I use the box. Are there any tips on how to make it better. I don't want a high tech expensive grow box and I don't havbe a lot of free time I just want to make my sucess rate for cloning to agar a little higher as now I do all my innoculations in open air.
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odium33
_____///
Registered: 05/16/06
Posts: 1,187
Loc: mycoland
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Re: Simple glove box [Re: jellyfish]
#6463712 - 01/15/07 07:54 PM (17 years, 2 months ago) |
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i would run a bead of silicon on the lid to make a seal that you dont have to duct tape everytime. i would also cut a 2" hole and stuff with polyfill in one of the corners to let the oust and lysol escape. other then that your golden
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YuckMan
Student
Registered: 11/28/06
Posts: 69
Last seen: 7 years, 4 months
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Re: Simple glove box [Re: odium33]
#6463726 - 01/15/07 07:59 PM (17 years, 2 months ago) |
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Silicon sounds good.
I used foam tape to seal mine. It's just as cheap as a roll of duct tape, and you shouldn't have to retape it everytime. Can be found at any hardwarestore.
http://www.citylivingstores.com/cgi-bin/item/174334
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Simple glove box [Re: YuckMan]
#6463756 - 01/15/07 08:07 PM (17 years, 2 months ago) |
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No. You don't need tape, silicon or anything else. A glovebox only needs to break the wind, not be sterile. There is no reason or need to seal one up tight. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Yep,the idea is still air it doesn't need to be sterile,give everything a spray down with bleach solution a few minutes before inoculation and you should be fine
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cpw1971
Mr
Registered: 10/07/06
Posts: 5,615
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Re: Simple glove box [Re: Blutjager]
#6464252 - 01/15/07 10:21 PM (17 years, 2 months ago) |
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i just layed mine on its side and taped some clear plastic over the hole and cut a few holes in the clear plastic. easy
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Magash
Da Bud Guru
Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Re: Simple glove box [Re: cpw1971]
#6464348 - 01/15/07 11:03 PM (17 years, 2 months ago) |
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Ok, my turn. Put a fish tank pump in a filter box (very easy to make) then hook it up to the glove box. Bad stuff can't float in if sterile air is always pumping out
-------------------- All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature? Join us at the Growery!
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cpw1971
Mr
Registered: 10/07/06
Posts: 5,615
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Re: Simple glove box [Re: Magash]
#6464545 - 01/16/07 12:49 AM (17 years, 2 months ago) |
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I wonder if I could just cut a hole big enough for my air purifier exhaust to fit in and tape or silicone it?? It has a hepa filter and neg ion generator. glovebox flowhood all in one??
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etam
it's a hobby...
Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 5 months
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Re: Simple glove box [Re: Magash]
#6464908 - 01/16/07 05:24 AM (17 years, 2 months ago) |
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Quote:
Magash said: Ok, my turn. Put a fish tank pump in a filter box (very easy to make) then hook it up to the glove box. Bad stuff can't float in if sterile air is always pumping out
That's a good idea. I think I'll do that. I'm well overdue on building a glove box.
I currently don't even use a glove box. I've always been wary of them because of the exploding oust phenomenon when you're flaming your needle :P. I do everything in open air and rarely have contams, but I really think I'm just very lucky.
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: Simple glove box [Re: etam]
#6464980 - 01/16/07 06:37 AM (17 years, 2 months ago) |
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Quote:
etam said:
Quote:
Magash said: Ok, my turn. Put a fish tank pump in a filter box (very easy to make) then hook it up to the glove box. Bad stuff can't float in if sterile air is always pumping out
That's a good idea. I think I'll do that. I'm well overdue on building a glove box.
I currently don't even use a glove box. I've always been wary of them because of the exploding oust phenomenon when you're flaming your needle :P. I do everything in open air and rarely have contams, but I really think I'm just very lucky.
I have experienced the phenomenon you speak of and I will say it loud and clear IT IS HORRIBLE and it detonates like a bomb I have never since had anything flammable in the glovebox with the exception of an alcohol prep pad to wipe needles down with,I rarely even flame needles,Just put everything you will need in the glovebox and spray a little bleach solution,and than use the alcohol pad to wipe the needle down every few jars,It works and it sure as hell beats going up in flames again,nothing ever really needs to be flammable in your glovebox
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Simple glove box [Re: Blutjager]
#6465117 - 01/16/07 08:16 AM (17 years, 2 months ago) |
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I strongly recommend against anything in a glovebox that causes the air to move, whether filtered or not. Sterility is irrelevant in a glovebox. If you want sterile(relatively) air, get a laminar flow hood. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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RoosterCogburn
Fearless,one-eyed U.S.Marshall
Registered: 08/25/06
Posts: 8,508
Loc: Dirty South, NJ
Last seen: 12 years, 6 months
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Yeah, if "sterile air" is pumping out, it HAS to be sucking outside air in somewhere, therefore it's not sterile.
The best design I saw was the clear rubbermaid simply turned upsidedown with some holes cut out for hands... KISS!
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13shrooms
Lightning Shaman
Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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i just got a rubbermade tub and simply want to cut 2 arm holes in the side and b done. my question is how or what do i use to cut this plastic? every time i use a razor knife the plastic cracks and shaters. tubs are not cheap when you have to keep buying them to start over. HELP!
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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ABC
Stranger
Registered: 11/16/08
Posts: 1,439
Last seen: 1 year, 11 months
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Re: Simple glove box [Re: 13shrooms]
#9572941 - 01/08/09 07:13 PM (15 years, 2 months ago) |
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Quote:
13shrooms said: i just got a rubbermade tub and simply want to cut 2 arm holes in the side and b done. my question is how or what do i use to cut this plastic? every time i use a razor knife the plastic cracks and shaters. tubs are not cheap when you have to keep buying them to start over. HELP!
1st, please dont revive old threads
what you should do is get/build an alcohol burner, heat up a butter knife and let it melt through the plastic. I was quite frustrated by cracking too. but a red hot knife works very well
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13shrooms
Lightning Shaman
Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Simple glove box [Re: ABC]
#9572970 - 01/08/09 07:17 PM (15 years, 2 months ago) |
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ok next time I will open a new post so we have more crap to look through during a search. but thanx ill melt it.
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Quote:
RogerRabbit said: No. You don't need tape, silicon or anything else. A glovebox only needs to break the wind, not be sterile. There is no reason or need to seal one up tight. RR
Ill back that up, i have a similar glovebox. The lid is a 2 peice hinged lid that slots together when closed, like a trapdoor. All i did was cut 2 holes for my arms. No gloves to seal it, just open holes. Works great.
I do use latex gloves that get wiped with alcohol.
That glovebox gave me 100% success compared to 90% failure without it.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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So this isnt in my gallery so i thought, this is a good time to upload.
This is my glovebox. Its just something i had lying around at the time, and thought yup that will do.
I badly cut the arm holes, not having a drill, hacksaw or anything. I had to heat a knife up and slow melt the arm holes. Its a realy bad job, but it works great.
all i do with this glovebox is clean it with soapy water, leaving the walls damp, transfer everything i need inside, wait 10 minutes then carry out my procedures.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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RoosterCogburn
Fearless,one-eyed U.S.Marshall
Registered: 08/25/06
Posts: 8,508
Loc: Dirty South, NJ
Last seen: 12 years, 6 months
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Re: Simple glove box [Re: ABC]
#9576041 - 01/09/09 09:07 AM (15 years, 2 months ago) |
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Quote:
ABC said:
Quote:
13shrooms said: HELP!
1st, please dont revive old threads
Reviving an old thread for real help IS BETTER than starting a new thread... Reviving old threads for no reason is iffy...
Onto your solution.
Simply save an old metal coffee can... heat the edge and melt out perfect circles.
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johnny_poke
Brownian movement
Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 6 months
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I used an el-cheapo soldering iron to cut my arm holes. I also siliconed 2 pieces of drain pipe in them to attatch my gloves to. Lid sealed with foam insulating tape (the kind used round doors and windows). I was not happy with the idea of opening the box lid to transfer my stuff from the PC so I did this: Cut the bottom off of a tupperware container, cut a corresponding hole in the side of the glovebox then siliconed in place. A simple and effective air tight door. In fact, my box is so airtight that my alcohol burner only lasted 5 mins before dying from lack of oxygen! I think I will just alcohol wipe my innoc. loop in future because I am afraid that the burners demand for oxygen may have created a vacuum and sucked impure air into the box. Hope this helps
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
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bcboy
30% larger in real life
Registered: 09/25/08
Posts: 111
Last seen: 5 months, 2 hours
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For perfect arm holes pick up a can of tomatoes or any can that has the diameter that will fit your arms. Empty the cans contents, and wearing oven mitts heat the outer edge red hot with a gas stove or propane torch and use this like a cookie cutter to make perfect arm holes. A coat hanger bent in a circle can also work.
Then grab yourself some tvek coveralls and cut the arms off at the spot they are a little large than the diameter of the arm holes. Then using a glue gun secure them from the outside direct to the plastic tub.
Cut a large viewing window in the top and use some overhead projector film to make a nice strong clear window. Tape that from the inside using duct tape or gorilla tape and your good to go.
for gloves just wear some latex gloves.
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