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OfflineEnki
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Liquid Culture Questions
    #6122061 - 10/01/06 06:32 PM (17 years, 5 months ago)

Hello, im attempting to do my first liquid culture and just have a few basic questions, what is the optimal temperature to create optimum growth? Would it do to just have it on top of a fridge in a closet ( wrapped in two tshirts), with a bandaid covering the hole??

I put about two cc's in each jar with distilled water mixed with light malt extract.

It has chunkies in it when i agitate it, but when its still all the stuff settles at the bottom.

any info will help thanks.


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Imagination is the one weapon in the war against reality.
Jules De Gaultier

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InvisibleBlutjager
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Re: Liquid Culture Questions [Re: Enki]
    #6122188 - 10/01/06 07:23 PM (17 years, 5 months ago)

Make a new one and scrap the band aid for micopore tape,as far as temp goes they will grow just fine at room temperature

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OfflineEnki
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Re: Liquid Culture Questions [Re: Blutjager]
    #6122233 - 10/01/06 07:35 PM (17 years, 5 months ago)

i don't have any micropore tape, why wont a bandaid work?


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Imagination is the one weapon in the war against reality.
Jules De Gaultier

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Offlinezathan
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Re: Liquid Culture Questions [Re: Enki]
    #6122331 - 10/01/06 08:03 PM (17 years, 5 months ago)

Probably because band-aid is airtight.

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OfflineEnki
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Re: Liquid Culture Questions [Re: zathan]
    #6122413 - 10/01/06 08:28 PM (17 years, 5 months ago)

if only the middle is covering the hole in the lid ( which isn't sticky..) its not airtight and allows airflow. This wasn't supposed to turn into an argument..


--------------------
Imagination is the one weapon in the war against reality.
Jules De Gaultier

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OfflineCantiSama

Registered: 10/21/04
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Re: Liquid Culture Questions [Re: Enki]
    #6122748 - 10/01/06 09:43 PM (17 years, 5 months ago)

this doesn't look like an argument, but you did say "any info will help" so maybe you shouldn't disregard someone else's experience.


--------------------
Tiger got to hunt, bird got to fly;
Man got to sit and wonder 'why, why, why?'
Tiger got to sleep, bird got to land;
Man got to tell himself he understand.

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OfflineEnki
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Re: Liquid Culture Questions [Re: CantiSama]
    #6123123 - 10/01/06 11:55 PM (17 years, 5 months ago)

i agree, i apologize for my hasty irratibility. It is appreciated.

comparing the protein that settles in the bottom of a jar with malt in it, does the growth from the spores also settle at the bottom when let sit?


--------------------
Imagination is the one weapon in the war against reality.
Jules De Gaultier

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Offlinesoulsizzle
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Registered: 05/17/05
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Re: Liquid Culture Questions [Re: Enki]
    #6123149 - 10/02/06 12:11 AM (17 years, 5 months ago)

for the record, micropore tape is cheap and available at Wal-Mart.


and LC grows just fine at room temperature.


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