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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Anonymous
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Post deleted by Anno
#6060072 - 09/14/06 08:59 AM (17 years, 7 months ago) |
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Edited by werdnaeroom8 (09/14/06 09:03 AM)
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Tweexican
Clit Commander
Registered: 11/06/03
Posts: 657
Last seen: 5 years, 9 months
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Re: LC examination (PICTURES) [Re: ]
#6060090 - 09/14/06 09:05 AM (17 years, 7 months ago) |
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They look perfect. As to why you have had contamination resulting from these, I have no clue. I was once told by someone that shooting hydrogen peroxide into an lc might kill bacteria and be beneficial prior to extracting mycelium for inoculation.
It could have been your jars as well as the needle used to extract the culture. Retry your work and see again.
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NizzyJones
Fight evil with funk
Registered: 08/22/06
Posts: 2,082
Loc: Somewhere North of Normal
Last seen: 1 year, 11 months
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Re: LC examination (PICTURES) [Re: Tweexican]
#6060126 - 09/14/06 09:14 AM (17 years, 7 months ago) |
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Sounds like the contam was during inoculation. Make sure to double double check your sterilization procedures both in making your jars and innoculating them.
-------------------- Wildflower seed on the sand and stone, may the four winds blow you safely home Curriculum vapidum (dry herb vapes)
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Anonymous
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Post deleted by Anno [Re: NizzyJones]
#6060147 - 09/14/06 09:19 AM (17 years, 7 months ago) |
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Edited by werdnaeroom8 (09/14/06 09:21 AM)
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rollypolly
mycologist intraining
Registered: 09/13/06
Posts: 40
Last seen: 17 years, 2 months
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Re: LC examination (PICTURES) [Re: ]
#6060226 - 09/14/06 09:56 AM (17 years, 7 months ago) |
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Im having the same problem. I use a "flowhood" a bigazz holmes hepa on top of my glovebox. I completely seal my lc jars and use an airport. My wbs jars have a double filter wiht spacers and silicone inoc ports. It has to be the syringe. I was doing the boiling method and got contames. So I wrote to wronguy and he suggested boiling as such and wraping the whole syringe with a paper towel soaked in alcohol then shoot all the water into the sink and let cool. Then suck up some lc. Will be trying this later today. I only have one empty syringe so pcing everytime would be quite tedious, but have heard from several people that you can get emptys from walmart. What section are they in? Word if you could post back to let me know how its coming along. Maybe Ill just have to bite the pc bullet
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mushnoon
MushNoob
Registered: 05/13/06
Posts: 1,080
Last seen: 8 years, 2 months
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Re: LC examination (PICTURES) [Re: rollypolly]
#6060245 - 09/14/06 10:08 AM (17 years, 7 months ago) |
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ummm the syringes you get at walmart are usually insulin syringes, not going to work. Go to a feed store like tractor supply co. Syringes there are only like 79cents
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Anonymous
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Post deleted by Anno [Re: rollypolly]
#6060262 - 09/14/06 10:17 AM (17 years, 7 months ago) |
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NizzyJones
Fight evil with funk
Registered: 08/22/06
Posts: 2,082
Loc: Somewhere North of Normal
Last seen: 1 year, 11 months
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Re: LC examination (PICTURES) [Re: ]
#6060482 - 09/14/06 11:39 AM (17 years, 7 months ago) |
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Also: Don't use lysol - it's been known to cause malformations. Use isopropyl alcohol or hydrogen peroxide.
-------------------- Wildflower seed on the sand and stone, may the four winds blow you safely home Curriculum vapidum (dry herb vapes)
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cookeman
Live and let live
Registered: 10/26/05
Posts: 1,077
Last seen: 11 years, 10 days
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Re: LC examination (PICTURES) [Re: ]
#6060495 - 09/14/06 11:43 AM (17 years, 7 months ago) |
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LC's don't show signs of contam. They may look fine, but it sounds like both were contamned from the same syringe used to start them up. You basically have to test out your LC on a couple half pints or so to know if they're any good or not. Usually LC's are pretty contam resistant on their own, but not if they're injected with contamned spores.
-------------------- “Let’s put it this way – to lump psychedelic mushrooms into the same group as methamphetamine is like lumping the Bible into the same group as Mein Kampf. I mean shit; they’re both books, right?” Joe Rogan R.I.P. - "Bones" - One of the greatest people I've ever had the pleasure of getting to know and become friends with.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: LC examination (PICTURES) [Re: ]
#6060529 - 09/14/06 11:56 AM (17 years, 7 months ago) |
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they look fine...
BUT I always tell people to use a test jar before inoculating a ton of jars up...
that way you don't end up with a ton of contaminated jars!~
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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