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Invisiblejellyfish

Registered: 10/02/05
Posts: 7,457
Agar Agar peroxide
    #5881840 - 07/20/06 03:57 PM (17 years, 7 months ago)

Does anyone ahve any experience using hydrogen peorixde with agar? I have some petri dishes that have cooled for a day and would it be possible for me to add some peroxide to them now? I want to do a liquid culture and then transfer some mycellium to the agar agar. How much should I use and is there any chance it will kill the mycellium?

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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Agar Agar peroxide [Re: jellyfish]
    #5881851 - 07/20/06 04:00 PM (17 years, 7 months ago)

What do you have in the petri dishes besides agar agar?
RR


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Agar Agar peroxide [Re: RogerRabbit]
    #5881860 - 07/20/06 04:02 PM (17 years, 7 months ago)

You need to add the peroxide before the agar solidifies, but after it cools down a bit. It should not harm the mycelium, maybe just stunt it a little. But it also may not be nessasary..


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Invisiblejellyfish

Registered: 10/02/05
Posts: 7,457
Re: Agar Agar peroxide [Re: RogerRabbit]
    #5881881 - 07/20/06 04:10 PM (17 years, 7 months ago)

It's just agar agar and malt extract.

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Offlineaccesstwo
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Registered: 04/29/06
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Re: Agar Agar peroxide [Re: jellyfish]
    #5881908 - 07/20/06 04:25 PM (17 years, 7 months ago)

no need to use peroxided agar if the lc is sterile. all you have to do is suck up some my into the syringe from the lc, let most of the myc settle to the bottom of the syringe then squirt 1cc into the agar. peroxide wont hurt it though there will be slightly slower growth then normal

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Invisiblefastfred
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Re: Agar Agar peroxide [Re: accesstwo]
    #5882949 - 07/20/06 09:59 PM (17 years, 7 months ago)

I recently picked 6 tissue samples from an oyster mushroom and put them on a 1.5% malt extract, .5% dextrose agar plate.

All 6 of them developed bacterial contamination within a day or two, so to save the plate I poured some 3% H2O2 on it and let it sit for about 10 minutes, then poured it off.

That was two or three days ago and the dish seems to be recovered and bacteria free. So that's what I would do, and I would only use peroxide if your dish becomes contaminated.

Peroxide doesn't last that long so I would only use it after you have a problem and only for a short period of time.


-FF

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