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Gr33nTree73
Registered: 05/23/06
Posts: 4,095
Loc: 585/843
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Sterlization?
#5739100 - 06/11/06 06:44 PM (17 years, 9 months ago) |
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ok pretty soon i should be getting a psilocybe cubensis spore syringe from one of my friends and will be trying to grow the shrooms using a 1/2 pint jar and making a mix of brown rice flower and what not. i want to know if boiling the jar for an hour instead of using a pressure cooker (bc i dont have one) works just as good. some ppl have had infected shrooms using this but i wonder if its bc they didnt let the jar cool off long enough. Some help please???
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toole
white-thumb (Onewhackmycophiliac)
Registered: 05/01/06
Posts: 500
Loc: spore #1203 - bas 2.34 - ...
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You can boil it;
it's called pasteurizing.
It's not as recommended as using a PC, as a PC is limited as well-it still does a better job.
But you can do it.
Make sure there isn't too much water in the pot, make sure the lid is sealed well. Boil it for like an hour to an hour an half? Maybe, someone might have to verify that.
*edit* You can do it pot-in-pot style, place a small pot in a larger pot of water. Put a towel or such between the jar and the smaller pot and put the lid on
GL
-------------------- -the adventures of suse and prescott.9- ..and the neverending triscut of doom !
Edited by toole (06/11/06 06:49 PM)
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cubensisfrenzy55
Stranger
Registered: 04/13/06
Posts: 204
Last seen: 17 years, 6 months
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Re: Sterlization? [Re: toole]
#5739152 - 06/11/06 06:56 PM (17 years, 9 months ago) |
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what he said, I used a big ass canner cooker and boiled for an hour and have only gotten one contam out of like 36. It works fine just remember to be super sterile during the inoculation period.
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beavis190
(.Y.)'s
Registered: 01/25/06
Posts: 432
Loc: lost in my head
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Re: Sterlization? [Re: toole]
#5739153 - 06/11/06 06:56 PM (17 years, 9 months ago) |
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boiling will work. boil for about 1 an a half to 2 hours and u should be good just be extra sterile when inoculating it.
-------------------- Confucius Says ... Man who put cock in Peanut Butter jar is Fucking Nuts. Man with tool in woman mouth May not necessarily be dentist. Schoolboy who play with schoolgirl during wrong period, get caught red-handed. He who fish in other's hole often catch crabs. Man who go to sleep with itchy butt, wake with smelly fingers... Man young when he snatches kisses, old when he kisses snatches.
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toole
white-thumb (Onewhackmycophiliac)
Registered: 05/01/06
Posts: 500
Loc: spore #1203 - bas 2.34 - ...
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Re: Sterlization? [Re: beavis190]
#5739164 - 06/11/06 06:58 PM (17 years, 9 months ago) |
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And you think to yourself, what is the key to a good sterile inoculation technique, well
Check this out:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5668914/an/0/page/0
-cheap -easy -clean
I use this method, 0 contams so far and I'm just getting a PC tomorrow
-------------------- -the adventures of suse and prescott.9- ..and the neverending triscut of doom !
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FooMan
Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Sterlization? [Re: toole]
#5739228 - 06/11/06 07:18 PM (17 years, 9 months ago) |
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Quote:
toole said: You can boil it;
it's called pasteurizing.
Negative Batman. Boiling is sterilizing, not pasteurizing. Boiling will work for PF cakes, but not whole grains. You should boil for at least an hour. PC's are needed for whole grains because endospores aren't effectively killed at regular boiling temps.
-------------------- Quick WBS Prep
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shroomerite
Apprentice
Registered: 06/09/06
Posts: 513
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Re: Sterlization? *DELETED* [Re: FooMan]
#5739603 - 06/11/06 09:05 PM (17 years, 9 months ago) |
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Post deleted by shroomeriteReason for deletion: k
-------------------- "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR
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figment
Apothecary
Registered: 01/04/06
Posts: 522
Loc: Quidity
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Yeah, it is sterilizing. But from my experience you should not use BRF. Takes too long and the fruits kind of suck, plus they are VERY suseptible to contams. Use WBS to hpoo...or just WBS. Trust me. Shit, I'm sure I'm not the only one who feels this way.
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Gr33nTree73
Registered: 05/23/06
Posts: 4,095
Loc: 585/843
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Re: Sterlization? [Re: figment]
#5740899 - 06/12/06 08:08 AM (17 years, 9 months ago) |
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ok thnx guys looks like i havea better idea of what i want to do, just might cost more money though
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Atheist
Stranger
Registered: 01/24/06
Posts: 13,705
Loc: USA
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Just find the PF tek and follow it exactly
it tells you everything you need and what to do
oh and read a shitload
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FooMan
Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Sterlization? [Re: figment]
#5749147 - 06/14/06 10:17 AM (17 years, 9 months ago) |
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Quote:
figment said: Yeah, it is sterilizing. But from my experience you should not use BRF. Takes too long and the fruits kind of suck, plus they are VERY suseptible to contams. Use WBS to hpoo...or just WBS. Trust me. Shit, I'm sure I'm not the only one who feels this way.
BRF contams easier then WBS? That's news to me.
Whole grains are just easier for bulk applications. If you don't intend to grow a bunch of mushrooms, PF cakes are fine. If you are interested in bulk and can't afford a PC, then by all means, cook up a bunch of PF cakes. They can just as easily be spawned to a bulk substrate.
-------------------- Quick WBS Prep
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tallgreen
chillin like avillain
Registered: 05/21/06
Posts: 293
Last seen: 17 years, 6 months
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Re: Sterlization? [Re: FooMan]
#5749177 - 06/14/06 10:31 AM (17 years, 9 months ago) |
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What about whole grains in the microwave? I have heard some people recommend against this, and I have never tried it. But I was considering doing this with Wild Bird Seed. Microwaves work by shooting out microwave radiation at the frequency that water resonates at, thereby vibrating the individual water molecules, which is essentially heat. This is why dry things don't get hot in the microwave. I can't imagine any organism that could survive this process. Don't spores contain water? I'll be trying a microwave tek on some WBS before I drop $50+ on a pressure cooker.
-------------------- Nothing you can know that isn't known. Nothing you can see that isn't shown. Nowhere you can be that isn't where you're meant to be. It's easy. All you need is love. - The Beatles
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Sterlization? [Re: tallgreen]
#5749187 - 06/14/06 10:40 AM (17 years, 9 months ago) |
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> BRF contams easier then WBS?
No, figment is full of hpoo.
Microwave sterilization... Hmmm... I'll be excited to hear your results. However many people have tried it and the vast majority get complete failure.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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tallgreen
chillin like avillain
Registered: 05/21/06
Posts: 293
Last seen: 17 years, 6 months
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Re: Sterlization? [Re: tallgreen]
#5749202 - 06/14/06 10:50 AM (17 years, 9 months ago) |
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My confidence was fortified when I saw this post: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4493850/page/0/fpart/1/vc/1
He microwaves in multiple sessions and the last one for 40 minutes! I'll definitely post my results. I'll probably try it in about 2 weeks, when I free up some jars.
-------------------- Nothing you can know that isn't known. Nothing you can see that isn't shown. Nowhere you can be that isn't where you're meant to be. It's easy. All you need is love. - The Beatles
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FooMan
Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Sterlization? [Re: tallgreen]
#5750526 - 06/14/06 06:31 PM (17 years, 9 months ago) |
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Quote:
tallgreen said: My confidence was fortified when I saw this post: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4493850/page/0/fpart/1/vc/1
He microwaves in multiple sessions and the last one for 40 minutes! I'll definitely post my results. I'll probably try it in about 2 weeks, when I free up some jars.
I was just going to say to anyone thinking about microwaving to search posts by Blackout, you beat me to it.
Microwaving can definitely be done, but it takes alot of tweaking to get right. The reason you don't see many teks involving microwaves is that there is no standardization. Microwaves come in all different wattages and what would take one microwave 10 minutes to cook could take 1 in a more powerful model.
-------------------- Quick WBS Prep
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MustardMan
Peace Frog
Registered: 10/18/05
Posts: 970
Last seen: 5 years, 7 months
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Re: Sterlization? [Re: FooMan]
#5750811 - 06/14/06 07:34 PM (17 years, 9 months ago) |
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I use a large pot with a rag on the bottom, and let the water simmer (with a secure lid on) for an hour or so.
Never had any contams.
-------------------- Wild Psilocybe Ovoideocystidiata Cultivated Cubensis
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