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Offlineoptikal_trip
enthusiast

Registered: 06/20/01
Posts: 382
Loc: Toronto
Last seen: 16 years, 9 months
Microwave Sterilization?
    #564135 - 02/26/02 09:57 PM (19 years, 6 months ago)

I found this the other day and wonderd if anyone had used it with success?
_________

Microwave Sterilization Technique
Huge thanks to Cranny!
Thanks to this innovative technique, pressure-cooker sterilization may be a thing of the past. Why didn't someone think of this before? Well, here it is:


You will need to use PLASTIC containers (i.e Tupperware). Punch holes on the lids as per PF's method. Pre-sterilize the containers by placing them in a tray of water a few millimeters deep. Set the microwave for 8 minutes and verify that the power is on "high".

Carefully remove the containers and add the substrate.

Once again, place the containers back on the trays. Make sure that you have some water in the tray (just a few millimeters deep is enough). Don't forget that you need to have the innoculation holes punched even before pre-sterilization (otherwise you'll have a messy microwave to clean up when the substrate heats up).

Thats it! Let cool (for a few hours at least). Innoculate in the usual manner. The individual who brought this technique to life claims to have employed it succesfully....and I have no reason to doubt.
___________

What do you guys think? I think I may give it a shot with an extra B+ syringe I have.. I think I'll follow the guide lines, then when the substrate is colonized I'll throw a casing layer over it and see what happens.. any suggestions?


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They chopped her goddam head off right there in the parking lot. Then they cut all kinds of holes in her head and sucked the blood out. I think they were after the pineal gland.


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OfflineCynicalMagician
enthusiast
Registered: 08/28/01
Posts: 231
Last seen: 19 years, 4 months
Re: Microwave Sterilization? [Re: optikal_trip]
    #564314 - 02/27/02 12:28 AM (19 years, 6 months ago)

Suggestion: tell me how it works out. :wink: 


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----------------------

"Order some golf shoes," I whispered. "Otherwise, we'll never get out of this place alive. You notice these lizards don't have any trouble moving around in this muck - that's because they have ~claws~ on their feet."


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OfflineZorba
journeyman

Registered: 02/11/02
Posts: 77
Loc: Victoria, B.C.
Last seen: 19 years, 4 months
Re: Microwave Sterilization? [Re: optikal_trip]
    #564426 - 02/27/02 02:18 AM (19 years, 6 months ago)

What subrates does the author claim to have success with? And how long is the substrate put in the microwave for? Maybe you could post a link to the original text.

Cheers


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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,143
Loc: my room
Last seen: 2 months, 21 hours
Re: Microwave Sterilization? [Re: Zorba]
    #564438 - 02/27/02 02:37 AM (19 years, 6 months ago)

The substrate seems to be the PF substrate.

The method can be found in the Grow/Find section of the Shroomery.


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Offlineoptikal_trip
enthusiast

Registered: 06/20/01
Posts: 382
Loc: Toronto
Last seen: 16 years, 9 months
Re: Microwave Sterilization? [Re: Zorba]
    #564545 - 02/27/02 07:25 AM (19 years, 6 months ago)

"What subrates does the author claim to have success with? And how long is the substrate put in the microwave for? Maybe you could post a link to the original text. "

Well since it says that pressure cooker sterilization is a thing of the past I'm guessing all substrates.. And also since it says put the container in the microwave for 8 minutes it applies for both container and substrate.
PS. That is the original text, copied and pasted.


--------------------
They chopped her goddam head off right there in the parking lot. Then they cut all kinds of holes in her head and sucked the blood out. I think they were after the pineal gland.


Extras: Filter Print Post Remind Me! Notify Moderator Top
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