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Rikku69
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Registered: 05/05/06
Posts: 67
Last seen: 17 years, 8 months
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reproduction question
#5634474 - 05/15/06 07:32 PM (17 years, 10 months ago) |
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I just need some information cleared up.
When a mushrooms drops spores those spores are all genetically different and NOT all clones correct?
Do all the spores from a single batch have the same genetic possibilities? For example if I take a cap from a small shroom and a cap from a large shroom from opposite sides of the terrarium, does this mean both caps should be able to create all the same possibilites?
In one grow fact it recommends putting a whole live shroom into a food processer inorder to grind up the mycilliuem and then put that in a culture jar. What would the mycilliuem have genetically? That would just be all clones form the shroom it came from right?
Would it be favorable to use all clones if that were possible?
Is it possible that over time your own spore prints become "genetically polluted" thereby you can only create spore prints with "unfavorable phenotypes" (aka small and not potent), and what can be done about this fact?????
thanks for anyone with enough brain power to figure this out!! much appreciated
- the sexy Rikku
Edited by Rikku69 (05/15/06 07:33 PM)
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BPJohnny
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Re: reproduction question [Re: Rikku69]
#5634542 - 05/15/06 07:42 PM (17 years, 10 months ago) |
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ok this question requires an answer that is far to in-depth and long for a forum, first i will just say that a clone cannot be made from spores. spores will carry SOME characteristics of the mushroom it came from, that is how they have the major types of strains. however the characteristics you really care about, fruiting capabilities, colonizing capabilities etc are different throughout the substrains. for really good in depth info about how mushrooms "work" pick up a copy of "The Mushroom Cultivator" will answer all your questions
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skeletor
the dude
Registered: 09/06/05
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Loc: heaven on earth
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Re: reproduction question [Re: BPJohnny]
#5634566 - 05/15/06 07:46 PM (17 years, 10 months ago) |
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if you want perticular characteristics say really big fruits or good rizo growth you clone. spores is just a gereral set of genetics. a bigger mushroom has better odds to get the characteristics of big mushrooms but you are by no means garenteed this characteristic. using spores is like breeding but its much harder to the characteristics you are looking for to make a freaky kind of dog... i mean strain
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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Snaggletooth
Stranger in a Strange Land
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Re: reproduction question [Re: skeletor]
#5634578 - 05/15/06 07:47 PM (17 years, 10 months ago) |
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It never hurts to have a book or two...
-------------------- Atheist Chat
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creamcorn
mad scientist
Registered: 03/13/06
Posts: 2,962
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Re: reproduction question [Re: Rikku69]
#5634581 - 05/15/06 07:47 PM (17 years, 10 months ago) |
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Quote:
Rikku69 said: I just need some information cleared up.
When a mushrooms drops spores those spores are all genetically different and NOT all clones correct?
correct.
Quote:
Do all the spores from a single batch have the same genetic possibilities? For example if I take a cap from a small shroom and a cap from a large shroom from opposite sides of the terrarium, does this mean both caps should be able to create all the same possibilites?
every spore is different. think like a sperm or egg. the spores on a particular mushroom are going to have similar genetics, but not identical.
Quote:
In one grow fact it recommends putting a whole live shroom into a food processer inorder to grind up the mycilliuem and then put that in a culture jar. What would the mycilliuem have genetically? That would just be all clones form the shroom it came from right?
correct, this would be cloning.
Quote:
Would it be favorable to use all clones if that were possible?
it is possible, and if you isolate a good producing strain than its certainly favorable!
Quote:
Is it possible that over time your own spore prints become "genetically polluted" thereby you can only create spore prints with "unfavorable phenotypes" (aka small and not potent), and what can be done about this fact?????
nope. like i said, all spores are different. they're like starting over with a fresh strain. on the other hand, if you keep cloning a clone and so on, there's a phenomena called senesce - which basically means to grow old. as the mycelium continues to divide, generation of clone after generation, it begins to age and loses vigor. that can be avoided by using a "master culture" - that is to have your original clone stored on agar or in liquid culture, and always growing out from there so that everything is 2nd generation, rather than taking from the 2nd to make a 3rd, and then a 4th, etc. another way to slow it down is by changing up the food you feed the mycelium. this is why there's multiple recipes for agar, so it can be switched up between generations. there's tons more to read on the topic here and over in the advanced forum if you run a few searches.
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Rikku69
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Last seen: 17 years, 8 months
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Re: reproduction question [Re: creamcorn]
#5634646 - 05/15/06 07:58 PM (17 years, 10 months ago) |
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So if a person could obtain a good spore syringe and just make spore prints (and not clone) there wouldn't be this "secense" and good batches of shrooms could be created indefinitly?
Should only 1 single spore print be used in a syringe or should a few different caps be used in a syringe? But just one single cap from one single shroom should be enough each time right?
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BPJohnny
Stranger
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Re: reproduction question [Re: Rikku69]
#5634682 - 05/15/06 08:05 PM (17 years, 10 months ago) |
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1 print can make many syringes
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creamcorn
mad scientist
Registered: 03/13/06
Posts: 2,962
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Re: reproduction question [Re: Rikku69]
#5634690 - 05/15/06 08:07 PM (17 years, 10 months ago) |
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yep, every time you use a spore syringe you're starting from the beginning - like giving birth to a child who will some day reproduce and have its children. and in fact, what you've really got is a number of different strains, there will be a couple at each inoculation point. this is why many of the mushrooms that result from multispore will each have their own unique set of genetics.
you can make many many syringes from a single cap. there would be no harm in using more than one cap, but it would be wasteful. some make as many as 20-30 or more syringes from one good sized dark print.
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Rikku69
Stranger
Registered: 05/05/06
Posts: 67
Last seen: 17 years, 8 months
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Re: reproduction question [Re: creamcorn]
#5635020 - 05/15/06 09:09 PM (17 years, 10 months ago) |
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Ok I get ya.
Thanx everyone for the info.
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