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Offlinehomusubi
Curious Noob =P
Male

Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 14 years, 1 month
Liquid Medium, sugar concentration??
    #5542841 - 04/22/06 12:19 AM (17 years, 11 months ago)

hi ppl!... some time ago i try, for the 1st time, to inoculate some PF cakes and some grain jars... 2 weeks in incubator and i haven't see anything =(
i suppose i have failed in my mission...

i don't know why they haven't been colonized by anything, not even bacterias or other fungus...

so i was thinking, maybe the spores aren't alive!! they traveled with me from Holland, so maybe they got killed be the heat in the car...

now, to see if they are alive, i will try to cultivate in some kind of solid medium (250ml dH2O + 5g gelatin + 5g Glucose) and i wanna try on a liquid medium also. I have read some topics, and i will try honey tek, because the material for it are at home :wink:

but now comes my question... do u need a 4% sugar solution? so, will 4g glucose in 100ml dH2O also work? and why 4%? what happens if u use more than 4%? and finally, and most important, is it enough with only sugar?

i also read, that it should be 4g sugar in 1L dH2O... but thats 10times less than 4%... that why I'm a bit confuse
thx for ur help :wink:

Edited by homusubi (04/22/06 12:25 AM)

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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Liquid Medium, sugar concentration?? [Re: homusubi]
    #5542937 - 04/22/06 12:53 AM (17 years, 11 months ago)

You can use MORE than 4% but you run into the solution being too thick. The myc becomes difficult to get into a syringe.

I believe that the 4% thing is mostly related to volume. In our case it is usually measured in ml not L. Not sure where you got that from, perhaps a typo somewhere.

4 ml honey -> 96ml water.

I use less for the sake of 'ease of use'. But 4% by VOLUME will be fine.

Have you checked out the sponsors section? Maybe you will be better served by buying new, fresh, spore syringes from a vendor rather than dinking around with some random spores you had in your pocket from holland or whatever.  :smile:

I recommend http://www.ralphstersspores.com/

Secure, reasonably fast, high quality, RELIABLE.

So far 100% viable.

Be sure to read the entire site carefully to cash in on his specials.

To me it's funny, his watered down SOTM syringes are darker than the competitors regular syringes..


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.

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Offlinehomusubi
Curious Noob =P
Male

Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 14 years, 1 month
Re: Liquid Medium, sugar concentration?? [Re: cappa]
    #5543025 - 04/22/06 01:32 AM (17 years, 11 months ago)

jajaja... oki..thx for ur help...

I don't remember where, but in this forum, some guy said:
4g of Dextrose in 1000ml water, thats why I'm asking...

the bad part of my story, is that I'm poor and don't have a credit card to buy more spores, if u wanna buy a syringe for me, u will have a fan club here in Chile :wink:

the print I carry from Holland, are from the magic mushroom gallery, Amsterdam...


--------------------
"Entonces, mientras camines por esta tierra, Tu y tus hijos abrazar?is las Tinieblas, beber?is s?lo sangre, comer?is s?lo cenizas, ser?is como fuisteis en el momento de morir, nunca muriendo, siempre viviendo. Caminar?is para siempre en las Tinieblas, todo cuanto toqu?is se desmoronar?, hasta los ?ltimos d?as".

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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Liquid Medium, sugar concentration?? [Re: homusubi]
    #5543033 - 04/22/06 01:41 AM (17 years, 11 months ago)

ah.. dextose. Can't help ya there bud, I know nothing of using dextrose.

If you're using honey, 4% by volume.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.

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Offlinehomusubi
Curious Noob =P
Male

Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 14 years, 1 month
Re: Liquid Medium, sugar concentration?? [Re: cappa]
    #5543301 - 04/22/06 08:17 AM (17 years, 11 months ago)

does someone knows, why u cant had to much honey/sugar?? i think there must be a limit, i haven't see any colonization on pure honey... so there must be a limit, not?





Quote:

cappa said:
ah.. dextose. Can't help ya there bud, I know nothing of using dextrose.

If you're using honey, 4% by volume.




ok, thx! :wink:

Edited by homusubi (04/22/06 08:26 AM)

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OfflineEquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 9 months
Re: Liquid Medium, sugar concentration?? [Re: homusubi]
    #5543438 - 04/22/06 09:31 AM (17 years, 11 months ago)

If you want to be successful, maybe you should do it like so many other people do; then, when you've got that down, try experimenting with other stuff...


--------------------
HELP!!!!!!!!!

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Offlinehomusubi
Curious Noob =P
Male

Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 14 years, 1 month
Re: Liquid Medium, sugar concentration?? [Re: EquilibriuM]
    #5543472 - 04/22/06 09:46 AM (17 years, 11 months ago)

Quote:

EquilibriuM said:
If you want to be successful, maybe you should do it like so many other people do; then, when you've got that down, try experimenting with other stuff...




so? how do many ppl do then?? considering i don't have, and cant get, malt extract, and with some effort i maybe can get agar...
so, how should i do, without that 2 things?

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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 6 months
Re: Liquid Medium, sugar concentration?? [Re: homusubi]
    #5543930 - 04/22/06 12:07 PM (17 years, 11 months ago)

100% honey is too thick there are tons of spores in it but nothing grows make a 4% honey solution cover it and let it sit out. youll see stuff grow pretty quick thats just what was already hanging out in the honey waiting.


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.

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Offlinemogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 4 months
Re: Liquid Medium, sugar concentration?? [Re: skeletor]
    #5543977 - 04/22/06 12:22 PM (17 years, 11 months ago)

25% Karo is the highest I tried. Slow starter, but thick grower. Must have been some hyphael town meetings trying to figure out how to colonize that high a concentration. But once they figured it out, growth was impressive.

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Offlineshroomsynergy
Fungi Freak
Male

Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 6 months
Re: Liquid Medium, sugar concentration?? [Re: mogur]
    #5543999 - 04/22/06 12:30 PM (17 years, 11 months ago)

I've been using 2 1/2 -3 teaspoons Karo per 200 ml of springwater, and get staggering results, but with honey a little less seems to work just as good for me. i dunno, but alot of the recomended mixtures grew myc quick, but not much total growth. now i get the bottle/jar AT LEAST 1/2 full of myc. (thats just me tho, i dunno if it'll work the same for you.


--------------------

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Offlinehomusubi
Curious Noob =P
Male

Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 14 years, 1 month
Re: Liquid Medium, sugar concentration?? [Re: shroomsynergy]
    #5544090 - 04/22/06 12:59 PM (17 years, 11 months ago)

one little detail... what is karo?? =P


--------------------
"Entonces, mientras camines por esta tierra, Tu y tus hijos abrazar?is las Tinieblas, beber?is s?lo sangre, comer?is s?lo cenizas, ser?is como fuisteis en el momento de morir, nunca muriendo, siempre viviendo. Caminar?is para siempre en las Tinieblas, todo cuanto toqu?is se desmoronar?, hasta los ?ltimos d?as".

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Offlinecaboomers
Stranger
Registered: 07/11/05
Posts: 157
Last seen: 15 years, 2 months
Re: Liquid Medium, sugar concentration?? [Re: homusubi]
    #5544108 - 04/22/06 01:09 PM (17 years, 11 months ago)



corn syrup, look for the baby bottle tek, easy, quick, works.. fill too 100 ml, suck out 8 ml of water, put back in 8 ml of karo, pc 20 mins or so..ive got real nice and thick myc using just plain water lc from cakes or grain to the baby bottle tek, works nicee good luck

Edited by caboomers (04/22/06 01:17 PM)

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Offlineshroomsynergy
Fungi Freak
Male

Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 6 months
Re: Liquid Medium, sugar concentration?? [Re: caboomers]
    #5544186 - 04/22/06 01:48 PM (17 years, 11 months ago)

yup, i have better results when i double the ammount of karo tho. i dunno. i like Karo better thanhoney, but its harder to tell when all of the nutrients are used up. Just remember to put a marble or something in your jar to stir  and break up the chunks of mycelium or it'll be a bitch to suck up with your syringe.
:psycow:


--------------------

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Offlinehomusubi
Curious Noob =P
Male

Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 14 years, 1 month
Re: Liquid Medium, sugar concentration?? [Re: shroomsynergy]
    #5544379 - 04/22/06 02:59 PM (17 years, 11 months ago)

a nice... thx :wink:
buuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuuutttttttttttttttttttt i cant buy it here, i live in Chile, and u don't see it in supermarkets... :'(

im making MEA at this moment... at night i will make some honey water, and try it...

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Offlineshroomsynergy
Fungi Freak
Male

Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 6 months
Re: Liquid Medium, sugar concentration?? [Re: homusubi]
    #5544413 - 04/22/06 03:18 PM (17 years, 11 months ago)

try here to order online!!
http://www.pastrychef.com/Catalog/karo_light_corn_syrup_3591695.htm

they ship to chile

...please dont freak mods...  just tryin' to help. :grin: :grin:


--------------------

Edited by ShrOOmSynEgry (04/22/06 03:19 PM)

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Offlinehomusubi
Curious Noob =P
Male

Registered: 04/09/06
Posts: 94
Loc: Concepcion, Chile
Last seen: 14 years, 1 month
Re: Liquid Medium, sugar concentration?? [Re: shroomsynergy]
    #5544594 - 04/22/06 04:43 PM (17 years, 11 months ago)

Quote:

ShrOOmSynEgry said:
try here to order online!!
http://www.pastrychef.com/Catalog/karo_light_corn_syrup_3591695.htm

they ship to Chile

...please dont freak mods...  just tryin' to help. :grin: :grin:




thx for ur dedication man :wink:
but I'm poor, and don't have a credit card :frown:
i just have 1 spore print, and 4 home made syringes, and a lot of will to learn ;D

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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Liquid Medium, sugar concentration?? [Re: shroomsynergy]
    #5545187 - 04/22/06 08:08 PM (17 years, 11 months ago)

CORN SYRUP. Karo is just a brand name.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.

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