Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDaCultivater
Stranger
Registered: 10/08/05
Posts: 289
Last seen: 15 years, 11 months
spore print to agar... soak or no soak?
    #5440224 - 03/25/06 02:26 AM (17 years, 11 months ago)

so i've read bolth and plan to do the sp to agar tomarrow...

so what do you guys think i should do, soak or not soak the spores first?

will the spore still germinate without soaking?
ty for any advise

-cult


--------------------
as they said in some movie(i don't remember which one);

a belief is a dangerous thing, people will kill over it; instead of believing you need to find your facts and then have an opinionated idea that you are willing to change in light of new evidence

Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: spore print to agar... soak or no soak? [Re: DaCultivater]
    #5440428 - 03/25/06 07:57 AM (17 years, 11 months ago)

No soak is needed.
There is plenty of moisture, in the agar, to germinate spores.


--------------------

Extras: Filter Print Post Top
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
Trusted Cultivator
Re: spore print to agar... soak or no soak? [Re: agar]
    #5440432 - 03/25/06 08:04 AM (17 years, 11 months ago)

Depending on how old the spores are they can take quite along time to rehydrate. Dehydrated spores will not germinate BTW. Most importantly if you are using relatively fresh spores (up to say 1yr.) you should be fine either way make sure you break the surface and deposit some of those spore "in" the agar this will be the first place germination will occur in most cases.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
Offlinebluemawkishness
Stranger
Male User Gallery

Registered: 03/17/06
Posts: 33
Loc: Switzerland
Last seen: 16 years, 4 months
Re: spore print to agar... soak or no soak? [Re: hyphae]
    #5440466 - 03/25/06 08:36 AM (17 years, 11 months ago)

I never soaked them so far, no probs. I just rasped some of the print on the agar.

If you have a very week print only, soaking can be helpful, because once you have a solution of the print in steril water, you can inoculate much more petris than by just rasping.

Edited by bluemawkishness (03/25/06 08:41 AM)

Extras: Filter Print Post Top
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
Trusted Cultivator
Re: spore print to agar... soak or no soak? [Re: bluemawkishness]
    #5440519 - 03/25/06 09:40 AM (17 years, 11 months ago)

Quote:

bluemawkishness said:
I never soaked them so far, no probs. I just rasped some of the print on the agar.

If you have a very week print only, soaking can be helpful, because once you have a solution of the print in steril water, you can inoculate much more petris than by just rasping.



I am only referring to old dehydrated spores BTW. Also even weak prints have plenty of spores the water is purely a means of re-hydration at this point. As far as on the surface or breaking the surface this was a tip from pro that has made my agar work easier than ever, using it or not it's up to the individual but I have to highly recommend it as it works and is a fine touch added to ones agar repertoire. I totally agree diluting spores in water will extend them but in agar IMHO it is simply redundant.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
Offlineimpgl
CrimethINCspecial agent
 User Gallery
Registered: 02/07/06
Posts: 2,462
Loc: california!
Last seen: 7 years, 5 months
Re: spore print to agar... soak or no soak? [Re: hyphae]
    #5440797 - 03/25/06 11:53 AM (17 years, 11 months ago)

so im unclear as to the germination process. you soak the old spores in distilled water, right? how much water and for how long? also, heres the part thats really a noodle scratcher for me: when in liquid form, how do i put it on the agar? thanks in advance!


--------------------
omg really?

Extras: Filter Print Post Top
InvisibleLiquidkick
H2O
Registered: 05/03/02
Posts: 2,635
Re: spore print to agar... soak or no soak? [Re: impgl]
    #5440895 - 03/25/06 12:28 PM (17 years, 11 months ago)

1 single drop of spore solution on the agar dish.

Extras: Filter Print Post Top
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
Trusted Cultivator
Re: spore print to agar... soak or no soak? [Re: impgl]
    #5440909 - 03/25/06 12:33 PM (17 years, 11 months ago)

First I would use a sterile syringes a sterilized shotglass and sterilized water. In a glovebox (still air) I would flame the needle or just wipe it down with alcohol and scrape some spores into the shotglass then inject the sterilized water from the syringe into the shotglass containing the spores and suck it back up let the syringe sit at room temperature for 1-2 weeks then in a sterile environment (GB) place a few drops on agar. How old are your spores BTW? Also a dirty print will leave you with many contams and isolating pure myc gets difficult.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
Offlineimpgl
CrimethINCspecial agent
 User Gallery
Registered: 02/07/06
Posts: 2,462
Loc: california!
Last seen: 7 years, 5 months
Re: spore print to agar... soak or no soak? [Re: hyphae]
    #5441069 - 03/25/06 01:24 PM (17 years, 11 months ago)

thanks for the info. its a p cube (Tazmanian) that i bought in amsterdam. completely sealed and prepped in a sterile lab (or so boasts the labeling). i got it around june of 04. whattaya think??


--------------------
omg really?

Extras: Filter Print Post Top
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
Trusted Cultivator
Re: spore print to agar... soak or no soak? [Re: impgl]
    #5441194 - 03/25/06 02:14 PM (17 years, 11 months ago)

Well you'll have plenty of spores if ya want to make some syringes and try spores directly to agar. I will recommend making at least one syringe at this time as this is as clean as that print will ever be. I would use a 1/2pt. jar and sterilize the water in that, use distilled water for sure as any other can germinate the spores because of the nutes in them. Remember all you need is a fraction of those spores to germinate and your in business. Remember when making the hole in the lid place it near the edge so you can get every last drop when tilted! :wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
OfflineDaCultivater
Stranger
Registered: 10/08/05
Posts: 289
Last seen: 15 years, 11 months
Re: spore print to agar... soak or no soak? [Re: hyphae]
    #5441327 - 03/25/06 03:07 PM (17 years, 11 months ago)

lol ty for th advise guys

very helpfull i shall do it TODAY!!!!
no soak
20 new dishes hear i come


--------------------
as they said in some movie(i don't remember which one);

a belief is a dangerous thing, people will kill over it; instead of believing you need to find your facts and then have an opinionated idea that you are willing to change in light of new evidence

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* How do you use spore prints efficently? dnkyrhubarb 20,202 14 03/03/22 04:07 PM
by Schtarker
* spore print recovery effort staindblue 727 4 07/03/02 05:02 PM
by staindblue
* Spore Prints
( 1 2 all )
DERRAYLD 5,011 20 06/01/19 06:02 PM
by Adden
* Using Agar medium directly from spore print?? djveiox 7,208 17 12/07/23 03:30 PM
by Screwup
* Spore print question ????? mojowilliams 1,908 10 01/04/02 10:31 AM
by ar393
* Attention Newbies-Make Spore Prints With Foil! Catzeye 22,342 1 02/20/02 01:54 PM
by psilocybinjunkie
* Using spore prints Droog 1,989 12 10/12/01 05:18 AM
by Droog
* Attention Newbies-Use Foil to make a sporeprint!
( 1 2 3 all )
Catzeye 25,740 46 02/23/02 12:20 PM
by bluhoney

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,075 topic views. 18 members, 90 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 12 queries.