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OfflineLochRaven
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trouble with spore prints on glass
    #5399507 - 03/14/06 02:59 PM (18 years, 19 days ago)

i recently started making my spore prints on the bottom of jars and have found it very difficult to get the spores off. any ideas?


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InvisiblePrisoner#1
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Re: trouble with spore prints on glass [Re: LochRaven]
    #5399513 - 03/14/06 03:03 PM (18 years, 19 days ago)

take a small piece of broken glass (sterile), drop it in, add sterile water, cap and agitate

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OfflineAkira
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Re: trouble with spore prints on glass [Re: LochRaven]
    #5399524 - 03/14/06 03:06 PM (18 years, 19 days ago)

I noticed the same thing. But, im still trying to get passed the "making the sryinge" part. I wiped my jars with alcohol and a paper towel, and my syringes appeared to have paper towel particles in them afterward :frown:


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Invisibleremediator
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Re: trouble with spore prints on glass [Re: Akira]
    #5399529 - 03/14/06 03:08 PM (18 years, 19 days ago)

Scraping with the sterile needle tip?

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InvisibleRoadkillM
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Re: trouble with spore prints on glass [Re: LochRaven]
    #5399545 - 03/14/06 03:11 PM (18 years, 19 days ago)

Quote:

LochRaven said:

i recently started making my spore prints on the bottom of jars and have found it very difficult to get the spores off. any ideas?




thats one of the problems with having prints in the bottom of jars...
they can be a pain in the ass to get off the bottom of the jar when making inoculant.

I like using Alum foil or glass microscope slides.


tc


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InvisiblemushboyMDiscord
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Re: trouble with spore prints on glass [Re: Roadkill]
    #5399589 - 03/14/06 03:21 PM (18 years, 19 days ago)

I just use 3x5cards cut in half. "sealed" in packing tape. cleaned with rubbing booze..put in glow box and make my print. when its done i put in a ziplock bag(cleaned in same manner) the first time i did this was with hawaiian. and i just cased those, and made another sryinge. I like this one because you can put use the neddle to scrap em off. i then shot clean water from syringe into bag(keeping spore card away from water)then suck back up. has worked for me about four times so far.

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InvisiblemushboyMDiscord
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Re: trouble with spore prints on glass [Re: Akira]
    #5399603 - 03/14/06 03:24 PM (18 years, 19 days ago)

Quote:

Akira said:
...and my syringes appeared to have paper towel particles in them afterward :frown:




this happens to me also!!  ive woundered how to fix that. just always wipe from the neddle base to the top in one motion then use lighter to burn away any fibers.

Edited by mushboy (03/14/06 03:27 PM)

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