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NumberThree
Jedi Drifter

Registered: 02/05/06
Posts: 263
Last seen: 12 years, 3 months

LC cooling time

#5389911 - 03/11/06 05:55 PM (18 years, 1 month ago)

Tomorrow I'm making up an LC and in one of the teks I'm reading up on it says to let the mixture cool overnight.

If I were to do this in the morning, would it be okay to inoclulate this by the end of the day, say late evening (5-9 pm)?

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NumberThree
Jedi Drifter

Registered: 02/05/06
Posts: 263
Last seen: 12 years, 3 months

Re: LC cooling time

[Re: NumberThree]
#5390100 - 03/11/06 07:04 PM (18 years, 1 month ago)

okay...

I guess Ill just wait the day out...

Edited by NumberThree (03/11/06 07:05 PM)

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skeletor
the dude

Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 7 months

Re: LC cooling time

[Re: NumberThree]
#5390141 - 03/11/06 07:20 PM (18 years, 1 month ago)

if its cool then you are fine. simple as that. should take an hour at most. unless your LC is giaganticcccccc

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RogerRabbit
Bans for Pleasure

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month

Re: LC cooling time

[Re: NumberThree]
#5390145 - 03/11/06 07:21 PM (18 years, 1 month ago)

When we say to let something cool overnight, the word 'overnight' is used in a generic sense to mean 'fully'. Your grains or culture medium cares little whether the sun or moon is shining outside, but your spores are going to perform poorly if you scrape them into very hot liquid.
RR

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JSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 5 months, 10 hours

Re: LC cooling time

[Re: skeletor]
#5390146 - 03/11/06 07:22 PM (18 years, 1 month ago)

i think 106F is the death point for spores. I put mine in front of a hepa blower to cool them faster

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skeletor
the dude

Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 7 months

Re: LC cooling time

[Re: JSshroom]
#5390501 - 03/11/06 09:53 PM (18 years, 1 month ago)

fridge or freezer in a ziplock if you are in a rush.

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JSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 5 months, 10 hours

Re: LC cooling time

[Re: skeletor]
#5395074 - 03/13/06 12:21 PM (18 years, 1 month ago)

it can break your jars though from cooling too fast

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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 5 months

Re: LC cooling time

[Re: JSshroom]
#5395083 - 03/13/06 12:24 PM (18 years, 1 month ago)

I pced myn for 25 minutes wich was to much but it still worked.
Then I let it cool for 45-70 mins then I ran luke warm water over it even though I still couldnt tuch the jar with bare hands then I lowerd and lowerd slowly the temp of the water. Then when It was about 70 degrees in the jar I inoculated with a chunk of a brf cake and then 5 days later inoculated 7 grain jars that are now 100% colonized with 0 contams!

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Chrissss
poke smoter

Registered: 02/12/06
Posts: 227
Last seen: 17 years, 3 months

Re: LC cooling time

[Re: mikeownow]
#5395210 - 03/13/06 01:11 PM (18 years, 1 month ago)

i put mine in the fridge and or freezer. works a lot faster.

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Razman
Stranger

Registered: 07/07/04
Posts: 514
Last seen: 9 years, 2 months

Re: LC cooling time

[Re: Chrissss]
#5395233 - 03/13/06 01:22 PM (18 years, 1 month ago)

no! stick to doing it overNIGHT! Daytime will actually REVERSE the process, and your jars will get so hot they'll explode. If you think a meth explosion is bad, you just wait n see an LC jar cool down the wrong way!

kidding :P

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skeletor
the dude

Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 7 months

Re: LC cooling time

[Re: Razman]
#5395569 - 03/13/06 03:19 PM (18 years, 1 month ago)

well im not saying to take the jars right out and put them in cold water... but after tehy have tooled enough to be handled putting them in the fridge or freezer won't explode them. i assure you.

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Get back to nature; hunting burgers and gathering fries.

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Herbus
...

Registered: 10/19/04
Posts: 1,477
Loc: Reading the map...
Last seen: 10 years, 3 months

Re: LC cooling time

[Re: skeletor]
#5395677 - 03/13/06 03:49 PM (18 years, 1 month ago)

I'd imagine LC cools alot quicker than WBS and BRF/Verm, it would seem much easier for the heat to disperse...

I highly doubt it would take "overnight" to cool a small batch of liquid culture...

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