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Offlinekasskalade
Stranger
Registered: 02/02/06
Posts: 2
Last seen: 18 years, 1 month
contamination problems!!!!
    #5263329 - 02/04/06 04:48 PM (18 years, 1 month ago)

huhhhh

every frekin time, excpet once, i get contamination by the same old conaminatnt. its black and spreads quicker then fire. it look like small black pinheads i think its Black Whisker Mold ? Doratomyces spp., based on my observations and the decription of it in the following link: http://www.shroomery.org/index.php/par/23418
when you touch it it turns powdery like sumjed black charcol or ahs on your hands.when i cleand my jars i scrubbed them with dishwashing soap and bleach afterwards. then i boiled them before putting the substrate in them. for the substrate i used distilled water, that was brought to a boil before mixing. i sterilized the PF jars in a pot of water for an hour and a half. as for the syringe, i sterlized it in boiling water, then scrubbed the needle with bleach and flamed it before inclonation. the jar are sealed and there is tape over the inclonation holes, and the incubator runs 86-88F and is a air tight tupperware container. does anyone know what this contam. is and how to get rid of it? ive lost many valuable spores and wasted much of my time with this going through the whole process only to end up with jars that have LOTS of contamination right when my spore start to germinate.HELP!!!

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OfflineStopGo
NothinShakin.....
Male

Registered: 11/27/05
Posts: 363
Loc: A Long Stange Trip
Last seen: 2 months, 25 days
Re: contamination problems!!!! [Re: kasskalade]
    #5263369 - 02/04/06 05:01 PM (18 years, 1 month ago)

My only advice is to invest in a pressure cooker, it will pay off in the end.


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Offlinekiss
numbnuts
 User Gallery

Registered: 12/14/04
Posts: 486
Loc: under your bed
Last seen: 9 years, 3 months
Re: contamination problems!!!! [Re: StopGo]
    #5263474 - 02/04/06 05:34 PM (18 years, 1 month ago)

I second that! A PC will be your most valued tool.


--------------------
see dick drink
see dick drive
see dick die
don't be a dick!

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Offlinebumper
Playin EVE
 User Gallery

Registered: 11/06/04
Posts: 498
Loc: Middle of Canada
Last seen: 11 years, 21 days
Re: contamination problems!!!! [Re: kasskalade]
    #5263748 - 02/04/06 07:18 PM (18 years, 1 month ago)

Yes get a pressure cooker, that and don't open them jars up and play with the mold, not only could you get sick but your letting the contamination out. If your gonna reuse your jars, fill them with bleach and let them sit for a day before opening, well thats what I would do to increase your chances of success.
Goodluck to you.


--------------------
Pitbulls are like any other dog, they can be trained to be nasty or they can be trained to be good, some are smart and some are stupid, just like every other breed.
Their tough, stubborn and loyal, which is easily turned into something horrible in the wrong hands.

Edited by bumper (02/04/06 07:30 PM)

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OfflineWronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 8 months
Trusted Cultivator
Re: contamination problems!!!! [Re: bumper]
    #5263822 - 02/04/06 07:34 PM (18 years, 1 month ago)

Read up on our contanimation information.

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Offlinebumper
Playin EVE
 User Gallery

Registered: 11/06/04
Posts: 498
Loc: Middle of Canada
Last seen: 11 years, 21 days
Re: contamination problems!!!! [Re: Wronguy]
    #5263842 - 02/04/06 07:37 PM (18 years, 1 month ago)

I dont wanna, it makes me sad. :frown:
:grin:

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InvisibleRahz
Alive Again
Male

Registered: 11/10/05
Posts: 9,285
Re: contamination problems!!!! [Re: kasskalade]
    #5264235 - 02/04/06 09:06 PM (18 years, 1 month ago)

A PC is the best investment you can make in this hobby. 30-40 bucks spent on a PC will make all the difference in the world. Your first PC doesn't have to be a real nice one, just make sure you can fit quarts in it cause if you stick with the hobby, you'll probably want to sterilize quarts at some point.

But, if you want to try your luck again, boil the jars, let them sit 24 hours and boil them again.

Rahz


--------------------
rahz

comfort pleasure power love truth awareness peace


"The object of opening the mind, as of opening the mouth, is to shut it again on something solid." - Gilbert Keith Chesterton

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OfflineRemainRandom50
Do You Need ToKnow Me?
Registered: 01/15/06
Posts: 1,695
Last seen: 14 years, 11 months
Re: contamination problems!!!! [Re: Rahz]
    #5266279 - 02/05/06 01:28 PM (18 years, 1 month ago)

yea defiently invest into a PC.


--------------------
At times I get consumed by my everyday life and will leave the Shroomery. Yet, every time drugs come falling into my life for fun.....I always think about the Shroomery and then I'm back!

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