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Offlineblackout
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Bow down to the Holy LC
    #5186069 - 01/16/06 03:17 AM (18 years, 2 months ago)

This is a thick SA LC. Just thought the pic looked nice, it is hard to get decent shots of LC, backlighting seems to work sometimes.

This was injected into some barley that was microwaved until it started to roast. I added water to 120g of barley and boiled up for about 15mins in total. I injected 120ml of LC and it was all absorbed 8hrs later. I am not sure if the LC is still viable though.

Edited by blackout (01/16/06 03:20 AM)

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Invisibleohmatic
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Re: Bow down to the Holy LC [Re: blackout]
    #5186099 - 01/16/06 03:31 AM (18 years, 2 months ago)

now THAT is a lotta mycelium in that syringe,
looking lovely bro !
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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Offlineblackout
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Re: Bow down to the Holy LC [Re: ohmatic]
    #5186134 - 01/16/06 04:54 AM (18 years, 2 months ago)

I did the LC in a jar with no filter, just 2 self healing holes. It was a mixture of apple juice and glucose. This allowed me to shake it very well, frothing it up well and aerating it, growth was very fast and easy to fragment the myc. After a week I added more concentrated glucose to the jar to start further growth. In theory this could be done over and over until you have a huge amount of myc, some said there would be a limit when the metabolites would poison the myc. It all floated to the top of the LC so I could suck it out from there giving me the extra thick myc concentration.

I put a filtered syringe in one hole and the other needle in the other. First I suck in just air and expel it several times, this draws and expels filtered air into the jar, then I suck in the LC, the 60ml of air is sucked in through the filter so there is no vacuum.

Edited by blackout (01/16/06 04:55 AM)

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Offlinedjsage420
aka DJQBNSIS
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Re: Bow down to the Holy LC [Re: blackout]
    #5186144 - 01/16/06 05:03 AM (18 years, 2 months ago)

Hey can you post your ratio of juice to glucose and where did you get your glucose?


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If you put your head to the grass, you can hear it growing

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Offlineblackout
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Re: Bow down to the Holy LC [Re: djsage420]
    #5186172 - 01/16/06 05:32 AM (18 years, 2 months ago)

Apple juice is about 11% sugar. You could add 1 part juice to 2 parts water to get about 4%. In this case I added 100ml of apple juice to about 800ml of water and 19g glucose. I then microwaved it and boiled it down to 750ml which gives 4% sugar. (100x11%=11g+19g=30g sugar in 750ml).

I get the glucose (anhydrous dextrose is also listed on the packet) in the baby food section in the supermarket.

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Offline_OttO_
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Re: Bow down to the Holy LC [Re: blackout]
    #5186182 - 01/16/06 05:46 AM (18 years, 2 months ago)

Nice pic - nominated... :thumbup:

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Invisibleohmatic
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Re: Bow down to the Holy LC [Re: _OttO_]
    #5186410 - 01/16/06 09:30 AM (18 years, 2 months ago)

so, u just get your jar, drill in say 2 holes, silocone them.

then fill the jar with nute solution, inoculate and shake the shit out of it every day
or so for a week, then use an airport to "change" the air and use it ?

i cannot believe i havend thought about this straight out perfectly simple method myself!
your my official hero of the day!
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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Offlineblackout
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Re: Bow down to the Holy LC [Re: ohmatic]
    #5186427 - 01/16/06 09:39 AM (18 years, 2 months ago)

Yep, as simple as that.
This jar had a shard of glass too but it is not really needed since you can shake so hard. I puncture 2 holes about 1cm apart with a corkscrew, this allows me to put a single blob of silicone down on both sides of the lid covering both holes so it is very secure.

I think air exchange in LC's is over-rated. The main function of the airport/filtered syringe is to equalise the pressure when sucking out LC. My jar is about 1litre so has 250ml of air space, I think that is plenty of air/oxygen. After boiling a LC all the gases are driven out. I think the initial aeration after a very vigorous shaking gives a lot more oxygen to the myc than a simple filter which only gets a gentle swirl. I have had a lot of my recent LC's contam due to wetting of filters so have now ditched them. I am also trying the sealed jars out with WBS and barley.

Edited by blackout (01/16/06 09:41 AM)

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OfflineAkira
CosmicConsciousness
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Re: Bow down to the Holy LC [Re: blackout]
    #5186455 - 01/16/06 09:54 AM (18 years, 2 months ago)

Looks like a syringe full of man seed to me. Are you cloning humans? :shocked:


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Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh

Edited by GodsEntelechy (01/16/06 09:55 AM)

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OfflineRemainRandom50
Do You Need ToKnow Me?
Registered: 01/15/06
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Re: Bow down to the Holy LC [Re: Akira]
    #5186495 - 01/16/06 10:14 AM (18 years, 2 months ago)

very nice, may have to tell a friend to try this one out!


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At times I get consumed by my everyday life and will leave the Shroomery. Yet, every time drugs come falling into my life for fun.....I always think about the Shroomery and then I'm back!

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Offlineliquidclutter
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Re: Bow down to the Holy LC [Re: RemainRandom50]
    #21380066 - 03/08/15 07:39 PM (9 years, 23 days ago)

:awesome:


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InvisiblebodhisattaMDiscordReddit
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Re: Bow down to the Holy LC [Re: liquidclutter]
    #21380081 - 03/08/15 07:43 PM (9 years, 23 days ago)

Quote:

liquidclutter said:
:awesome:



#5186495 - 01/16/06 11:14 AM (9 years, 1 month ago)

:tard:

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Offlineliquidclutter
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Re: Bow down to the Holy LC [Re: bodhisatta]
    #21380113 - 03/08/15 07:51 PM (9 years, 23 days ago)

its a good picture


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InvisiblebodhisattaMDiscordReddit
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Re: Bow down to the Holy LC [Re: liquidclutter]
    #21380119 - 03/08/15 07:53 PM (9 years, 23 days ago)

:shrug:

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OfflineYaMoonSun
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Registered: 10/23/14
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Re: Bow down to the Holy LC [Re: liquidclutter]
    #21380200 - 03/08/15 08:09 PM (9 years, 23 days ago)

Quote:

liquidclutter said:
its a good picture



:lolsy:

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Offlinetourrat
humanbeinganimal
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Re: Bow down to the Holy LC [Re: YaMoonSun]
    #21380352 - 03/08/15 08:41 PM (9 years, 22 days ago)

Yeah I've been using baby bottles that are totally sealed with no gas exchange and I'm getting EXPLOSIVE growth using wbs soak water. Gas exchange is way over rated for lc.


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Knee deep in the hotel tub.....

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InvisiblebodhisattaMDiscordReddit
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Re: Bow down to the Holy LC [Re: tourrat]
    #21380361 - 03/08/15 08:42 PM (9 years, 22 days ago)

lots of air is disolved in water and in the headspace. normally hot water doesn't hold air but it had nowhere to go if you had a tight lid on in the PC run so that's why.

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Offlineliquidclutter
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Re: Bow down to the Holy LC [Re: tourrat]
    #21380417 - 03/08/15 08:56 PM (9 years, 22 days ago)

Quote:

tourrat said:
Yeah I've been using baby bottles that are totally sealed with no gas exchange and I'm getting EXPLOSIVE growth using wbs soak water. Gas exchange is way over rated for lc.




are u a troll?

Quote:

agar said:

This is a picture of an LC with tubing type gas exchange filters.

It was not agitated.

Simply left alone in an incubator.

There is a healthy big ball of myc in the solution.
(50/50 light dry malt & dry dextrose)

That is not the point.

The point is that myc grows thick & rapidly on the surface.
Which, (IMHO) proves that gas exchange in an LC leads to optimal growth.




Quote:

agar said:
Any decent LC - with gas exchange.
Left un-agitated.
Will sooner or later get a myc mat on the surface.

Which is not a really GOOD thing.
(unless you have a powerful stir plate & bar combo)

Because, the mat gets thick, spongy & will PLUG syringes.
(because the mat becomes a fairly solid mass)


This is an LC, that is just beginning to form a mat.


This is a THICK LC, that was stirred every time a mat began to form.


This is what happens when, after a mat is formed, & you stir the LC.
Mat is one tough SOB to break up.


This is about optimal mass in an LC.




wait....

Quote:

total said:
Pc'ed 3 baby bottles last night using Agar's tek but using his Turbinado Lc recipe.
threw alcohol soaked rags over the pcs valves and intakes while it cooled down trying to be as sterile as possible. open up and tightened them once they got room temp. Only one made a seal a complete airtight seal though...the other 3 leaked water when pressed on nipple :frown:

Just gave her a minisquirt off a Z strain syringe i made.  Was no vacuum in there and it didnt wanna suck my syringe, so i must have let it cool long enough.  Hope there is enough air in there for growth and works out.  Will keep posted.






ill take my 2 shrooms now


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Offlineblackout
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Re: Bow down to the Holy LC [Re: liquidclutter]
    #21393643 - 03/11/15 03:59 PM (9 years, 20 days ago)

sorry to bump this old thread again, but just to make it clear, when I said "I think air exchange in LC's is over-rated" I meant the need for filters on jars. I believe aeration of the liquid is important, and you do not need a filter to get a well aerated liquid, I believe you will get relatively good aeration in a partially full jar, like 1/4 or 1/2 full which is shaken well.

I believe there is plenty of air in the head space of a sealed LC jar. If you were to strain off a regular LC it probably has far less myc in it than a jar full of grains. Many are not good at making stuff or cannot source filters or do not want the expense.

If you have a large jar with a small amount of LC there you do not even need a secondary hole, you will be able to suck out LC and it will create a vacuum in the jar. If all you need is say 30ml and you jar has 400ml of free head space then you should be able to draw this out. In a 200ml LC I might only bother sucking out 30ml, since if you let it settle properly and can tilt the jar you can suck out thick myc and leave clear liquid behind, the lack of a filter makes this tilting easier/safer.

Even with 1 hole and a fairly full jar it can be done. You pull out 10ml in a syringe, inject it in your jars and now flame your needle until red hot, now put the flame at the end of the needle and very slowly draw in air, this should be clean air but will have all the oxygen burnt up. You then inject this into your jar and the vacuum in the jar will suck that air back in, then suck more air out and repeat. This is another risk though, so I would sooner use large jars with less liquid.

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