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Offlinebadmotofinger
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Registered: 08/30/02
Posts: 39
Last seen: 18 years, 3 months
Single Jar of Rye
    #4925542 - 11/12/05 11:45 AM (18 years, 4 months ago)

AFOAF was given one quart of fully spawned rye, ready to go. Her luck w/ casings being lousy, she wants to do a few different things with it. She will innoc more grain jars with some of it for future use, no prob there, but she also wants to try to make some liquid myc by adding some of the grains to a sterile solution of karo syrup @ distilled water for use with a PF project. My thought is that the rye will bring contams to the solution. She thought that after 48 hours of grain introduction, she could remove the grain and add h2o2 to the solution, this warding off contams.

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Invisiblebackupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: Single Jar of Rye [Re: badmotofinger]
    #4925611 - 11/12/05 12:12 PM (18 years, 4 months ago)

sounds like she should do some more research before attempting such things.
as long as the cultures were made from a healthy jar and all sanitary procedures are taken and work done inside a glovebox then it shouldn't be a problem.
not so sure her idea of adding and then trying to remove is a good idea. lc's are very prone to contams and shouldn't be messed with in such ways.

:peace:

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InvisibleMezcal
Registered: 08/11/05
Posts: 1,980
Re: Single Jar of Rye [Re: badmotofinger]
    #4925690 - 11/12/05 12:43 PM (18 years, 4 months ago)

Add some distilled H2O to the colonized grain, let it flow through the mycelia, suck it up with a sterile syringe and inject into LC. You'll have growth overnight.

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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
Last seen: 4 months, 21 days
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Re: Single Jar of Rye [Re: Mezcal]
    #4926739 - 11/12/05 07:06 PM (18 years, 4 months ago)

fruit it and clone

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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
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Re: Single Jar of Rye [Re: badmotofinger]
    #4926820 - 11/12/05 07:48 PM (18 years, 4 months ago)

First off... you most likely mean FULLY COLONIZED RYE.

Secondly, you most likely mean G2G, rather than "innoc".

Thirdly, unless you have a good still air G/B & are aways up the ladder on sterile procedures.  I would not be opening (taking the lid off) any LC. I have used myc to start an LC, but not with any grain of rye attached to it.

Fourthly, if it did work out for you, no sense opening the LC to remove the rye grain, as doing so would greatly increase your odds of contaminating it.

LC's are easy. :thumbup: But, unforgiving. :rolleyes: One tiny mistake, results in contaminate soup. :eek: :mad2:

If you do agar work?

Suggest, when you open that colonized rye jar. In a G/B. Transfer out a piece of myc into a prepared agar PDA plate (1/2 pint jar, BB food jar, petri, or whatever you use). Then incubate it.

Once colonization spreads & is healthy looking. Flood the plate with about 5 to ml of sterile water, agitate the surface of the plate with a sterile syringe needle, then tip the plate & aspirate a few ml into the syringe.

Then, use that solution to inoculate a prepared LC container.






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