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Limpet_Chicken
Stranger
Registered: 10/30/05
Posts: 43
Loc: England
Last seen: 9 years, 6 months
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Cyanescens on cardboard...likelyhood of success?
#4878737 - 11/01/05 08:01 AM (18 years, 4 months ago) |
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I am an absolute newb when it comes to woodlovers, my prev. experience is a couple of B+ Cubensis grows, and an Agaricus Bitorquis kit.
Now, something new for me, I just so happened to find a trip worth of Psilocybe Cyanescens, growing on rotting alder twigs, of course, I harvested those, and collected the spores in my dessicator as I dried the mushrooms over NaOH.
I have just attempted preperation of a simple mycelium culture, on sterrilised cardboard, with a makeshift, although completely sterile incubator, cleaned with first MeOH, then boiling water, likewise the spore syringe, MeOH rinse/fill, then rinsed with boiling water, sterile distilled water was used to make the spore solution.
Cardboard was sterrilised with boiling water, without alcohol.
How likely, is it, that perhaps I might be lucky enough to get the square of cardboard to colonise?
If they do, I will likely sterrilise the rest of the cardboard, and apply a little peroxide, followed to transfer of the mycelial culture onto as much cardboard as I have, and thence to a large woodchip bed located in some nearby quiet woods, and watered weekly, in the hope that a flush of cyans or two might pop up.
So, for those with prior experience (I.E more than me ), any chance that I might succeed in my endeavour? or is this doomed from the start? any reccomendations?
-------------------- The light blinds So behold darkness as our new light In our darkness we can see So with others blindness We take flight.
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thenewguy05
The Mushroom Man
Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 7 months
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Re: Cyanescens on cardboard...likelyhood of success? [Re: Limpet_Chicken]
#4878766 - 11/01/05 08:19 AM (18 years, 4 months ago) |
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i have grown azuresence with cardboard SPAWN, but i'm not sure if the cardboard has enough nutrients to promote fruiting.
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Limpet_Chicken
Stranger
Registered: 10/30/05
Posts: 43
Loc: England
Last seen: 9 years, 6 months
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Re: Cyanescens on cardboard...likelyhood of success? [Re: thenewguy05]
#4878874 - 11/01/05 09:24 AM (18 years, 4 months ago) |
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I didn't intend to attempt fruiting off cardboard, I was more wondering about likelyhood of colonising the cardboard, as that is what I am using as spawn, to inoculate a woodchip bed out in the woods.
-------------------- The light blinds So behold darkness as our new light In our darkness we can see So with others blindness We take flight.
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thenewguy05
The Mushroom Man
Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 7 months
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Re: Cyanescens on cardboard...likelyhood of success? [Re: Limpet_Chicken]
#4878915 - 11/01/05 09:38 AM (18 years, 4 months ago) |
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u should b fine although outdoor beds are best done in the spring.
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Exothermic
Stranger
Registered: 06/19/03
Posts: 49
Last seen: 18 years, 3 months
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Re: Cyanescens on cardboard...likelyhood of success? [Re: thenewguy05]
#4879055 - 11/01/05 10:22 AM (18 years, 4 months ago) |
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If you succeed in this, and i wish you luck, please do a grow log.Very interesting
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Limpet_Chicken
Stranger
Registered: 10/30/05
Posts: 43
Loc: England
Last seen: 9 years, 6 months
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Re: Cyanescens on cardboard...likelyhood of success? [Re: Exothermic]
#4880803 - 11/01/05 06:17 PM (18 years, 4 months ago) |
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I will do a growlog assuming I get anything at all.
Has anyone tried fruiting cyans indoors? an indoor bed would be pretty good if one were to devote a room to a grow
-------------------- The light blinds So behold darkness as our new light In our darkness we can see So with others blindness We take flight.
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Limpet_Chicken
Stranger
Registered: 10/30/05
Posts: 43
Loc: England
Last seen: 9 years, 6 months
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Re: Cyanescens on cardboard...likelyhood of success? [Re: Limpet_Chicken]
#4882503 - 11/02/05 03:21 AM (18 years, 4 months ago) |
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I neglected to mention, I did a second, earlier attempt in a sterillised coffee jar, on a substrate of wholemeal wheat flour (all that I had in the house) and boiled distilled water.
That was covered with two layers of cling film and kept quite wet, then inoculated with some spores from the Cyanescens prints I aqquired, the jar is now around 4 days old, and is beginning to get a cover of fluffy white mycelium.
I intend on letting the jar colonise, then transferring a specimen of the mycelium to several large casings of alder chips in icecream tubs, to try for an indoor grow, whilst saving the majority of the culture for inoculation of a several meter high hardwood chip pile nearby
-------------------- The light blinds So behold darkness as our new light In our darkness we can see So with others blindness We take flight.
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