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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,601
Loc: Oregon, USA
Last seen: 1 hour, 36 minutes
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Unidentified Bluing Panaeolus from Maryland, USA
#4798805 - 10/13/05 01:47 PM (18 years, 5 months ago) |
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Preliminary results from an unidentified specimen collected in June of 2005 from a field in Maryland.
Post documenting original collection
Original Collection and Dried Specimen #19 with closeup of bluing stem base.
Micrographs of Specimen #19. Gill Surface showing spores and cap cuticle showing large round cells. Pointer is 8.4 microns in width.
Bluing mycelium isolated from gill fragments and fully colonized grass seed mixed with a small amount of horse manure.
Collection date: June 2005 Cap: Parabolic to hemispheric, light brown to tan. Stem: Equal to more narrow at base which is often curved, same color as cap with whitish mycelium at base which stains blue. Spores: Black, elliptical to lemon shaped on 4-spored basida Cap cuticle: Cellular Staining: Blue on Stem base, reportedly faint stains on cap margins. Habitat: Grassy Fields, Maryland Environmental Conditions: High heat and humidity
Genus: Panaeolus Species: Unknown at this time.
Fruiting attempt is underway. The mycelium is extremely aggressive and the spores harvested from the gills germinated and grew into visible mycelium in just 24 hours on MEA at 80F
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification
Edited by Workman (10/13/05 03:04 PM)
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4803120 - 10/14/05 11:24 AM (18 years, 5 months ago) |
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4-spored Not chlorocystis. Looking very interesting. Any pleurocystidia, or only cheliocystidia? Good luck with the fruiting.
That was really fast growth.
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,601
Loc: Oregon, USA
Last seen: 1 hour, 36 minutes
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: EonTan]
#4803304 - 10/14/05 12:43 PM (18 years, 5 months ago) |
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I have a hard time sectioning rehydrated material so I am hopeful to get some fruits. Fresh material looks so much better under the scope. I will look at the gill structures if I am successful with the cultivation. I cased yesterday and already the mycelium is breaking through. If it fruits as fast as it grows it could be a winner.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4817723 - 10/17/05 07:31 PM (18 years, 5 months ago) |
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Tweexican
Clit Commander
Registered: 11/06/03
Posts: 657
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: EonTan]
#4819558 - 10/18/05 04:06 AM (18 years, 5 months ago) |
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i'll trade you a legit print, for an unkown of these guys.
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Tweexican]
#4820169 - 10/18/05 10:58 AM (18 years, 5 months ago) |
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There not mine to trade, ask the source.
They look like Pan caster/greatonefolious complex more then they are a copelandia. With copelandias the pleurocystidia are impossible to miss. These look similar to the unknowns we get in florida, except the bluing looks more intense with this unknown. Very interesting and can't wait for the fresh specimens microscopy.
I had success growing a variant of the local florida unknown on mixed compost( farm manure/shreeded pine bedding). It is not a Pan.subb, or a copelandia. It even fruited pretty well outdoors when mixed with oak leaf litter and blak cow manure( Colonized compost>leaf litter/blak cow).
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,601
Loc: Oregon, USA
Last seen: 1 hour, 36 minutes
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: EonTan]
#4820950 - 10/18/05 02:21 PM (18 years, 5 months ago) |
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You may not have to wait long. Primordia are forming in the tray. They might be photoworthy tomorrow and mature sometime next week.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,601
Loc: Oregon, USA
Last seen: 1 hour, 36 minutes
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4825513 - 10/19/05 03:00 PM (18 years, 5 months ago) |
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Here are the little devils.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification
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mskip23
Can It All Be So Simple!
Registered: 06/08/05
Posts: 1,522
Loc: Philly
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4825585 - 10/19/05 03:14 PM (18 years, 5 months ago) |
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Nice
-------------------- url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif][/url
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stankness
BeaST
Registered: 06/09/05
Posts: 50
Loc: Here
Last seen: 17 years, 5 months
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: mskip23]
#4826969 - 10/19/05 08:47 PM (18 years, 5 months ago) |
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Those do resemble pan gg's, they look like they have a more distinguished color though, maybe a bit lighter. The blueing is by far more intense. Nice finds and sick pins man!
-------------------- Attention Floridian shroomers! Check out this site! Gainesville Pyilocybin Society
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,601
Loc: Oregon, USA
Last seen: 1 hour, 36 minutes
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4833982 - 10/21/05 12:51 PM (18 years, 5 months ago) |
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Today - 2 days later.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification
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GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4834452 - 10/21/05 02:06 PM (18 years, 5 months ago) |
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wow..you did it!
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,601
Loc: Oregon, USA
Last seen: 1 hour, 36 minutes
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4850826 - 10/25/05 03:25 PM (18 years, 5 months ago) |
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Cap: marginal band Gills: adnate Veil: absent Stem: pruinose (powdery) Basidia: definately 4 spored. Pleurocystidia absent. Cheilocystidia capitate. Spore print: black Spores: roughly 10 x 8 microns, smooth (as far as I can tell with this microscope).
Final identification:
Panaeolus subbalteatus group: A single variable species or a complex of closely related forms.
Aurora (Mushrooms Demystified) mentions a variant in California that matches well with this specimen.
Quote:
One variant (also hallucinogenic) that is common on fertilized lawns in our area has a slimmer stalk (1-3mm thick) and smaller cap. Whether it is a "new" species or merely a dimunitive form of P. subbalteatus is for the Panaeolus-pundits to decide.
Stamets (Psilocybin Mushrooms of the World) also describes perhaps the same species from coastal California under Panaeolus foenisecii.
Quote:
A form similar to P. foenisecii which occasionally bruises bluish near the base of the stem, has been collected in grassy areas in coastal California. This mushroom may either be an active variety of P. foenisecii or is a new species.
This collection has black spores and not the brown spores of P. foenisecii.
I will, for now, label it a small hallucinogenic grass land variety of Panaeolus subbalteatus.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification
Edited by Workman (10/25/05 03:27 PM)
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4850907 - 10/25/05 03:47 PM (18 years, 5 months ago) |
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magnificent identification photos
-------------------- buh
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_OttO_
Over Stimulated
Registered: 06/01/05
Posts: 2,588
Loc: Up Over
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4858364 - 10/27/05 07:18 AM (18 years, 5 months ago) |
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Inspiring...... Great stuff!
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MagicalMystery
turn off yourmind
Registered: 07/27/05
Posts: 1,740
Loc: Here, there and everywher...
Last seen: 18 years, 4 months
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Re: Unidentified Bluing Panaeolus from Maryland, USA *DELETED* [Re: Workman]
#4858855 - 10/27/05 11:12 AM (18 years, 5 months ago) |
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Post deleted by shirley knottReason for deletion: not welcome in any forum i moderate
-------------------- "Men trained in arms from their infancy, and animated by the love of liberty, will afford neither a cheap or easy conquest." From the Declaration of the Continental Congress "We can have peace and security only as long as we band together to preserve that most priceless possession, our inheritance of European blood." Charles A. Lindbergh,"Aviation, Geography, and Race", Reader's Digest, Nov. 1939 "We must secure the existance of our people and a future for White children." David Lane
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eris
underground
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Posts: 48,024
Loc: North East, USA
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4859070 - 10/27/05 12:20 PM (18 years, 5 months ago) |
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Very nice work there.. You are very good at cultivation and microbiology. The effort is much appreciated.
-------------------- Immortal / Temporarily Retired The OG Thread Killer My mushroom hunting gallery
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mikonn
me
Registered: 05/29/05
Posts: 192
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4866430 - 10/29/05 07:33 AM (18 years, 5 months ago) |
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I have to thank Workman for all of his hard work on this specimen. I'm just sorry that it didn't turn out to be something more interesting. I must admit that I am continuously amazed at the diversity and seeming adaptablity of "Subbs". They are real chameleons. I wonder what it is about their genetics that generate such varied macroscopic morphology (naturally, while still presenting a sufficent number of taxonomic determiners), while maintaining there microscopic characteristics. Perhaps this isn't unique in the broader scope of mycology, I'm admittedly a novice, and I understand as well that the "group" designation implies observed variances that are as yet unquantified. I did be interested in seeing a post of the variety of specimens that have been microscopically id'd as Panaeolus subbalteatus or it's various international pseudonyms. anyway. thanks again Workman. You again have demonstrated how valuable an asset you are to these boards!
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
#4872073 - 10/30/05 06:34 PM (18 years, 4 months ago) |
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Nice job, but I think it is a mistake to keep lumping this with subbalteatus.( better then foenisecii)
But where else does it go?
Great pics by the way. The cheliocystidia one in particular.
Compatability test are needed to put the definitive answer on the subb question.
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warpigs330
Stranger
Registered: 11/01/05
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: stankness]
#4880894 - 11/01/05 06:41 PM (18 years, 4 months ago) |
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I wouldn't mind a print of those.
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