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OfflineWorkmanV
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Unidentified Bluing Panaeolus from Maryland, USA
    #4798805 - 10/13/05 01:47 PM (18 years, 5 months ago)

Preliminary results from an unidentified specimen collected in June of 2005 from a field in Maryland.

Post documenting original collection


Original Collection and Dried Specimen #19 with closeup of bluing stem base.



Micrographs of Specimen #19.
Gill Surface showing spores and cap cuticle showing large round cells.
Pointer is 8.4 microns in width.



Bluing mycelium isolated from gill fragments and fully colonized grass seed mixed with a small amount of horse manure.

Collection date: June 2005
Cap: Parabolic to hemispheric, light brown to tan.
Stem: Equal to more narrow at base which is often curved, same color as cap with whitish mycelium at base which stains blue.
Spores: Black, elliptical to lemon shaped on 4-spored basida
Cap cuticle: Cellular
Staining: Blue on Stem base, reportedly faint stains on cap margins.
Habitat: Grassy Fields, Maryland
Environmental Conditions: High heat and humidity

Genus: Panaeolus
Species: Unknown at this time.

Fruiting attempt is underway. The mycelium is extremely aggressive and the spores harvested from the gills germinated and grew into visible mycelium in just 24 hours on MEA at 80F


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Edited by Workman (10/13/05 03:04 PM)

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InvisibleEonTan
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4803120 - 10/14/05 11:24 AM (18 years, 5 months ago)

4-spored
Not chlorocystis.
Looking very interesting. Any pleurocystidia, or only cheliocystidia?
Good luck with the fruiting.

That was really fast growth.

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OfflineWorkmanV
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: EonTan]
    #4803304 - 10/14/05 12:43 PM (18 years, 5 months ago)

I have a hard time sectioning rehydrated material so I am hopeful to get some fruits. Fresh material looks so much better under the scope. I will look at the gill structures if I am successful with the cultivation. I cased yesterday and already the mycelium is breaking through. If it fruits as fast as it grows it could be a winner.


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InvisibleEonTan
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4817723 - 10/17/05 07:31 PM (18 years, 5 months ago)

:thumbup:

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OfflineTweexican
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: EonTan]
    #4819558 - 10/18/05 04:06 AM (18 years, 5 months ago)

i'll trade you a legit print, for an unkown of these guys.


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InvisibleEonTan
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Tweexican]
    #4820169 - 10/18/05 10:58 AM (18 years, 5 months ago)

There not mine to trade, ask the source.

They look like Pan caster/greatonefolious complex more then they are a copelandia. With copelandias the pleurocystidia are impossible to miss. These look similar to the unknowns we get in florida, except the bluing looks more intense with this unknown. Very interesting and can't wait for the fresh specimens microscopy.

I had success growing a variant of the local florida unknown on mixed compost( farm manure/shreeded pine bedding). It is not a Pan.subb, or a copelandia. It even fruited pretty well outdoors when mixed with oak leaf litter and blak cow manure( Colonized compost>leaf litter/blak cow).

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OfflineWorkmanV
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: EonTan]
    #4820950 - 10/18/05 02:21 PM (18 years, 5 months ago)

You may not have to wait long. Primordia are forming in the tray. They might be photoworthy tomorrow and mature sometime next week.


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OfflineWorkmanV
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4825513 - 10/19/05 03:00 PM (18 years, 5 months ago)

Here are the little devils.



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Invisiblemskip23
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4825585 - 10/19/05 03:14 PM (18 years, 5 months ago)

Nice :thumbup:


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Offlinestankness
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: mskip23]
    #4826969 - 10/19/05 08:47 PM (18 years, 5 months ago)

Those do resemble pan gg's, they look like they have a more distinguished color though, maybe a bit lighter. The blueing is by far more intense. Nice finds and sick pins man!  :thumbup: :thumbup:


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OfflineWorkmanV
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4833982 - 10/21/05 12:51 PM (18 years, 5 months ago)

Today - 2 days later.



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InvisibleGGreatOne234
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4834452 - 10/21/05 02:06 PM (18 years, 5 months ago)

wow..you did it!

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OfflineWorkmanV
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4850826 - 10/25/05 03:25 PM (18 years, 5 months ago)



Cap: marginal band
Gills: adnate
Veil: absent
Stem: pruinose (powdery)
Basidia: definately 4 spored.
Pleurocystidia absent.
Cheilocystidia capitate.
Spore print: black
Spores: roughly 10 x 8 microns, smooth (as far as I can tell with this microscope).

Final identification:

Panaeolus subbalteatus group: A single variable species or a complex of closely related forms.

Aurora (Mushrooms Demystified) mentions a variant in California that matches well with this specimen.

Quote:

One variant (also hallucinogenic) that is common on fertilized lawns in our area has a slimmer stalk (1-3mm thick) and smaller cap. Whether it is a "new" species or merely a dimunitive form of P. subbalteatus is for the Panaeolus-pundits to decide.




Stamets (Psilocybin Mushrooms of the World) also describes perhaps the same species from coastal California under Panaeolus foenisecii.

Quote:

A form similar to P. foenisecii which occasionally bruises bluish near the base of the stem, has been collected in grassy areas in coastal California. This mushroom may either be an active variety of P. foenisecii or is a new species.




This collection has black spores and not the brown spores of P. foenisecii.

I will, for now, label it a small hallucinogenic grass land variety of Panaeolus subbalteatus.


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Edited by Workman (10/25/05 03:27 PM)

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Offlineshirley knott
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4850907 - 10/25/05 03:47 PM (18 years, 5 months ago)

magnificent identification photos  :thumbup:


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buh

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Offline_OttO_
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4858364 - 10/27/05 07:18 AM (18 years, 5 months ago)

Inspiring...... Great stuff!

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OfflineMagicalMystery
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Re: Unidentified Bluing Panaeolus from Maryland, USA *DELETED* [Re: Workman]
    #4858855 - 10/27/05 11:12 AM (18 years, 5 months ago)

Post deleted by shirley knott

Reason for deletion: not welcome in any forum i moderate



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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4859070 - 10/27/05 12:20 PM (18 years, 5 months ago)

Very nice work there.. You are very good at cultivation and microbiology. The effort is much appreciated.  :thumbup:


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Invisiblemikonn
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4866430 - 10/29/05 07:33 AM (18 years, 5 months ago)

I have to thank Workman for all of his hard work on this specimen. I'm just sorry that it didn't turn out to be something more interesting. I must admit that I am continuously amazed at the diversity and seeming adaptablity of "Subbs". They are real chameleons. I wonder what it is about their genetics that generate such varied macroscopic morphology (naturally, while still presenting a sufficent number of taxonomic determiners), while maintaining there microscopic characteristics. Perhaps this isn't unique in the broader scope of mycology, I'm admittedly a novice, and I understand as well that the "group" designation implies observed variances that are as yet unquantified. I did be interested in seeing a post of the variety of specimens that have been microscopically id'd as Panaeolus subbalteatus or it's various international pseudonyms. anyway. thanks again Workman. You again have demonstrated how valuable an asset you are to these boards!

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InvisibleEonTan
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: Workman]
    #4872073 - 10/30/05 06:34 PM (18 years, 4 months ago)

Nice job, but I think it is a mistake to keep lumping this with subbalteatus.( better then foenisecii)

But where else does it go?  :confused:

Great pics by the way. The cheliocystidia one in particular.

Compatability test are needed to put the definitive answer on the subb question.

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Offlinewarpigs330
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Re: Unidentified Bluing Panaeolus from Maryland, USA [Re: stankness]
    #4880894 - 11/01/05 06:41 PM (18 years, 4 months ago)

I wouldn't mind a print of those.

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