Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinesharppixy
Stranger
Registered: 02/27/05
Posts: 16
Last seen: 17 years, 2 months
consistent contam after innoc
    #4096381 - 04/25/05 05:51 PM (18 years, 11 months ago)

Eight cakes became contaminated right at the inoculation points within days of innoculating. In the first set of 4 cakes, the needle was flamed with a lighter and cleaned off with a paper towel soaked isopropanol. Two different syringes were used (one species per jar), all contams (looks like the beginnings of blue-green mold). Maybe the paper towel wasnt sterile enough. Thus, in the next set of 4 jars the needle was only flamed in a clean-burning alcohol flame. The needle never touched anything but the innoc. holes. The needle was flamed each time and left to cool for a few seconds before inserting into each hole. The area around the holes was wiped down with ethanol to sterilize the lids futher. But it looks like there are still comtams occuring. In all cases the holes have had tape on since PCing them, so contams couldn't really get onto the top of the vermiculite layer and get pushed down into the substrate when the needle is inserted. There are still two of the eight jars left... still waiting to see if EVERY innoc. point gets contaminated to try to figure things out. Is it possible for the syringes to become contaminated, even if one of them hadn't been used before? The only other thing it could be is that the environment is chock full of mold in the air... This contamination spree never happened when inoculation was attempted at another location with one of the same syringes. Could someone tell me why this happened?

Extras: Filter Print Post Top
Offlinejamsandwich
Male
Folding@home Statistics
Registered: 03/21/05
Posts: 328
Loc: Flag
Last seen: 8 years, 10 months
Re: consistent contam after innoc [Re: sharppixy]
    #4096852 - 04/25/05 07:45 PM (18 years, 11 months ago)

Sounds like there is something in the syringe to me, did you happen to ever pull back on the syringe and pull air in? Those sterility procedures seem like enought to prevent contams, so im guessing its what you are shooting in. Good Luck on the last 2.


--------------------
8 years now! lol

Extras: Filter Print Post Top
Offlinesharppixy
Stranger
Registered: 02/27/05
Posts: 16
Last seen: 17 years, 2 months
Re: consistent contam after innoc [Re: jamsandwich]
    #4098916 - 04/26/05 12:56 PM (18 years, 11 months ago)

Crap. The last two are contammed as well. There was some mycellium beginning to develop, but more blue green mold surrounding it. How did both syringes get contaminated? The syringes weren't pulled back after innoc., so it's hard to understand how contams got into the syringes... Next time there'll be new syringes and the old ones will still be around to test once more if they are really contammed (only on one or two cakes). Oh, and a more sterile environment too.

Extras: Filter Print Post Top
OfflineSuntzu
Geek
 User Gallery

Registered: 10/14/99
Posts: 1,396
Last seen: 18 days, 3 hours
Re: consistent contam after innoc [Re: sharppixy]
    #4101280 - 04/27/05 12:42 AM (18 years, 10 months ago)

Something to consider trying;  For the next batch, set aside two jars.  Keep one aside uninoculated.  Take the second and fake inoculate:  if you're working with two syringes, stick each needle in two of the four holes but don't push out any liquid.  Flame the needle and do everything the same as the real jars.  If the air is just really filthy [or the needle], the fake inoculated jars would be just as likely to contam as the real jars.  If only the real jars contaminate, the syringe fluid is likely mold-ridden. 
It's not a guaranteed answer, but probably worth trying after a 100% loss :frown:  The syringes can be replaced, but it would be good to know if the environment is actually the problem.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Possible way around contaminated shrooms?
( 1 2 all )
Juggles 9,462 20 06/23/01 05:01 AM
by madscientist
* Post deleted by Administrator HappyLeftist 946 8 01/08/03 01:27 PM
by roygbiv
* Contam'd by a mushroom!! Suntzu 1,050 2 09/09/01 11:43 PM
by Mitchnast
* Bright red/purple inky contamination oO_wombat_Oo 2,741 3 04/05/03 01:13 PM
by MAIA
* what kind of contamination is this ? (PIC) ragadinks 2,192 6 12/22/03 06:03 PM
by ragadinks
* Green specs, 20hrs after innoc.. dankcrop 1,401 5 10/27/02 10:23 AM
by Seuss
* all jars contam'd? boochaka 817 3 07/27/03 12:22 PM
by GregHimself
* Contaminated Rye Deviant 2,548 6 10/12/09 08:09 AM
by peshii

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,089 topic views. 9 members, 67 guests and 101 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 14 queries.