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NicerOo
Stranger
Registered: 04/05/05
Posts: 5
Last seen: 18 years, 10 months
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stalled colonization of jar?
#4019613 - 04/05/05 10:50 PM (18 years, 11 months ago) |
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The colonization of a jar seems to have stopped for over a week. It is not infected. It hasn't changed color or appearance or anything. Certain parts of the outside of the jar just aren't being taken over with mycellium. I think it might be because there is not enough moisture in the jar...is this a possibility? What course of action should be taken? Should more water be injected into the partially colonized jar?
Edited by NicerOo (04/05/05 10:51 PM)
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GNIOM1498
Death Cup
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Loc: My home is where my spiri...
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Re: stalled colonization of jar? [Re: NicerOo]
#4019623 - 04/05/05 10:55 PM (18 years, 11 months ago) |
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brf? im guessing then u could add sterile water to it but i wouldn't i tried it before just makes puddle. i would just cut the off whats not colonized.
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
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Crasher
αἱρετίζω
Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
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Re: stalled colonization of jar? [Re: GNIOM1498]
#4019662 - 04/05/05 11:07 PM (18 years, 11 months ago) |
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What temp, what substrate? How long was it colonizing before the stall? Does it smell funny?
-------------------- Give me silence, water, hope; Give me struggle, iron, volcanoes...
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NicerOo
Stranger
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Re: stalled colonization of jar? [Re: Crasher]
#4019823 - 04/05/05 11:54 PM (18 years, 11 months ago) |
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total darkness in the mid-high 70s...
this is for the formula reccomended in the newbie guide... for 1/2 pint canning jars (250 ml):
1/4 cup of brown rice powder 1/2 cup of vermiculite 45 - 60 ml (cc) water (1/4 cup = 59 ml)
it colonized for maybe 2 weeks before stalling...
it doesn't smell funny through the masking tape; risk of infection has kept the jar closed
should it be placed in indirect light?
Edited by NicerOo (04/05/05 11:54 PM)
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actionshroom
RookieMycologist
Registered: 10/15/04
Posts: 210
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stalled colonization of jar? [Re: NicerOo]
#4019835 - 04/05/05 11:56 PM (18 years, 11 months ago) |
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Take the tape off to allow oxygen into the jar.
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NicerOo
Stranger
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Re: stalled colonization of jar? [Re: NicerOo]
#4019842 - 04/05/05 11:58 PM (18 years, 11 months ago) |
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^^that won't contaminate it?
also, the jar was turned upside down after it stalled for a bit
Edited by NicerOo (04/05/05 11:59 PM)
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actionshroom
RookieMycologist
Registered: 10/15/04
Posts: 210
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stalled colonization of jar? [Re: NicerOo]
#4019860 - 04/06/05 12:03 AM (18 years, 11 months ago) |
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It's very unlikely it will contaminate. The vermiculite is a barrier for contaminants. In the past I have fully opened jars in non-sterile environments without contamination.
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Mindzpore
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Re: stalled colonization of jar? [Re: actionshroom]
#4020478 - 04/06/05 05:16 AM (18 years, 11 months ago) |
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i suppose it depends on how dirty your place really is and contaminant fallout at the place you are storing for colonization.
i've done the same as actionshroom and left jars without lid and also i almost never bothered with tape. contamination was very unusual (enough to surprise me whenever it happened). but then again... my place was relatively clean, i did all procedures in a glove box and my colonizations spot was very clean with no (very little) moving air.
-------------------- Mindzpores words of wisdom: "If you think something is foolproof, you just haven't met proper fools". Wiccan_Seeker said: "It is better to adjust to become a better listener than to keep on cranking up the volume".
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: stalled colonization of jar? [Re: NicerOo]
#4020518 - 04/06/05 05:56 AM (18 years, 11 months ago) |
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Pull the tape off...the verm barrier will protect the substrate.
The tape is what is stalling your jar...you have a CO2 build up.
The jar will take off again after you pull the tape off.
good luck!~
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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NicerOo
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Re: stalled colonization of jar? [Re: actionshroom]
#4020687 - 04/06/05 08:18 AM (18 years, 11 months ago) |
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how long should the tape come off for? until its ready for birthing?
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: stalled colonization of jar? [Re: NicerOo]
#4020771 - 04/06/05 08:46 AM (18 years, 11 months ago) |
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Quote:
NicerOo said:
how long should the tape come off for? until its ready for birthing?
Yep
your done with the tape now.
TC
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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jamsandwich
Registered: 03/21/05
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Re: stalled colonization of jar? [Re: Roadkill]
#4021364 - 04/06/05 11:29 AM (18 years, 11 months ago) |
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what is the point of having tape then?
-------------------- 8 years now! lol
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PawPaw
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Registered: 12/20/04
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Re: stalled colonization of jar? [Re: jamsandwich]
#4021375 - 04/06/05 11:33 AM (18 years, 11 months ago) |
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Quote:
jamsandwich said: what is the point of having tape then?
So you can tape stuff ...
-------------------- Come to Paw Paws place there will be no sleeping tonight Eric just wants to catch reds .. Don?t look back, don?t look back He?s right on your trail Don?t look back, don?t look back He?s just a step away from hell WDYWFM?
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Roadkill
Retired Shroomery Mod
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Re: stalled colonization of jar? [Re: jamsandwich]
#4021387 - 04/06/05 11:38 AM (18 years, 11 months ago) |
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Quote:
jamsandwich said:
what is the point of having tape then?
to keep the water out during sterilization.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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jamsandwich
Registered: 03/21/05
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Re: stalled colonization of jar? [Re: Roadkill]
#4021409 - 04/06/05 11:44 AM (18 years, 11 months ago) |
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i'm so glad i found this forum. its been 6 days and my jars have been taped the whole time. there is myc growth in 6 of the 8 jars, but now i'm hoping they will take off.
-------------------- 8 years now! lol
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: stalled colonization of jar? [Re: jamsandwich]
#4021417 - 04/06/05 11:46 AM (18 years, 11 months ago) |
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I pull the tape off my BRF/Verm jars when the mycelium is about the size of a Quarter or 50 cent pc. inside the jars.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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jamsandwich
Registered: 03/21/05
Posts: 328
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Last seen: 8 years, 10 months
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Re: stalled colonization of jar? [Re: Roadkill]
#4021429 - 04/06/05 11:48 AM (18 years, 11 months ago) |
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of the 6 that have myc, they are all about the size of a quarter. I'm hoping for the other 2 jars, but something may have gone wrong.
-------------------- 8 years now! lol
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: stalled colonization of jar? [Re: jamsandwich]
#4021450 - 04/06/05 11:53 AM (18 years, 11 months ago) |
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how many days has it been since you inoculated your jars?
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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NicerOo
Stranger
Registered: 04/05/05
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Last seen: 18 years, 10 months
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Re: stalled colonization of jar? [Re: Roadkill]
#4093842 - 04/24/05 11:58 PM (18 years, 10 months ago) |
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ok...it's been about 20 days since i took off the tape... about 12 days ago i injected some water into the cake because taking off the tape did nothing... i'm still really not seeing much of anything...
is my only option taking off the lid? do you really think this is what's holding it back? i'm very baffled o.O =(
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