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InvisibleMeltingPenguin
Pooh-Bah

Registered: 08/29/01
Posts: 2,138
Loc: new england
Agar mix?
    #396292 - 09/17/01 12:21 AM (20 years, 13 days ago)

I heard something about mixing agar with dog food instead of brewers malt and nut. yeast.
Has anyone had success useing this method? Is it worth it or should i just hold off till I find the yeast and malt?

I'll never look at the Tazmanian Devil the same way again.

Edited by MeltingPenguin on 09/17/01 12:44 AM.



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Growing anything is good for the soul


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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/18/99
Posts: 24,143
Loc: my room
Last seen: 2 months, 6 days
Re: Agar mix? [Re: MeltingPenguin]
    #396453 - 09/17/01 06:05 AM (20 years, 13 days ago)

"Working with agar can be an effective and rewarding approach to both microbiological culture and mycology. There are several different basic techniques that can then be modified and combined to suit the needs of your hobby. I've broken the basic subjects down into seperate pages and listed them in what I feel is a logical order. We begin with the agar preparation itself, then advance through the steps most folks are likely to need, in the order they will likely be encountered.

But first, how difficult is this really going to get? How easy or how hard is this going to be? Let's start with the most basic set up that is at all reasonably possible. Take a quart of tap water and heat it on the stove. Crush up roughly an ounce of any decent dog food as finely as possible. Mix an equal weight of plain agar into the dogfood. The agar you can get from any health food store will be just fine. Pour the hot water over the dog food and agar. Mix it well, and heat it a bit more until the agar is dissolved. Pour this mix 1/2 inch deep into the bottom of some wide mouth canning jars. The small the jars are the better. Put lids on the jars, and process them in a pressure cooker for 30 minutes at 15 psi. There, you are done. You have perfectly functional "petri dishes" that will grow a wide variety of micro-organisms. The most difficult thing about the whole process was likely using the pressure cooker. And let me assure you that many a grandmother has used a pressure cooker without having any sort of rocket science degree. Now, go get started! "

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because you will never, ever get it out.

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InvisibleMeltingPenguin
Pooh-Bah

Registered: 08/29/01
Posts: 2,138
Loc: new england
Re: Agar mix? [Re: Anno]
    #396903 - 09/17/01 06:13 PM (20 years, 12 days ago)

Anno rocks
thanks
peace

I think its dumb when people say video games effect kids negatively.
I mean, if Pac-Man effected us, we'd all hang out in dark rooms, muching pills, and listening to repetitive music.


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Growing anything is good for the soul


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InvisibleSumGuy
addict

Registered: 07/26/01
Posts: 556
Re: Agar mix? [Re: Anno]
    #408153 - 09/29/01 11:29 AM (20 years, 20 hours ago)

Anno can this agar be pc'd longer? A friend was thinking of killing two birds with one stone and pc'ing rye jars as well as agar at the same time.



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-SumGuy :cool:


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InvisibleNyahbinghi
Stranger

Registered: 09/29/01
Posts: 24
Loc: Mt. Zion
Re: Agar mix? [Re: SumGuy]
    #408633 - 09/29/01 11:44 PM (20 years, 8 hours ago)

DFA is a great media for getting fruitbodies *fast* and can save alot of time in isolating a pure strain from a multi-spore culture. Simply innoculate DFA with spores and culture tissue from one of the quickly forming fruitbodies onto fresh dishes of a less rich media such as MEA or PDA.

It is also a method to quickly obtain fresh spores. Cubensis carpophores on DFA sporulate normally and heavy, sterile prints are easily obtainable in a minimal amount of time.

Sumguy-- I suspect this media can withstand longer sterilzation since there is no malt to carmalize. This is not a media for medium or long term culture storage though since the media is so rich.

Edited by Nyahbinghi on 09/29/01 11:50 PM.



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Preservation through dissemination.
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