Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags, Substrate Mix   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 10 years, 4 months
Agar & Peroxide Question
    #3816983 - 02/22/05 01:26 PM (19 years, 29 days ago)

I was trying to follow the peroxide tek bit they but they dont have a ratio for peroxide to petri dish basis. I did a search and it wasnt there either. Anyone on the board with skills in the agar department know what a good ration for peroxide to agar in a petri dish would be. I have a 350 ml Bottle of RTP Nutrient agar and I now for a fact Im not going to use the whole entire bottle in one run so I think it would be a waste of time to peroxidise the whole damn bottle. Any suggestions??

-Odd :confused:


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??

Extras: Filter Print Post Top
Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 10 years, 4 months
Re: Agar & Peroxide Question [Re: oddiseus]
    #3817651 - 02/22/05 03:56 PM (19 years, 29 days ago)

Bump -Sorry- Just need some help.


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??

Extras: Filter Print Post Top
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Agar & Peroxide Question [Re: oddiseus]
    #3817673 - 02/22/05 04:01 PM (19 years, 29 days ago)

first, are you starting from spores or culture

Extras: Filter Print Post Top
Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 10 years, 4 months
Re: Agar & Peroxide Question [Re: Prisoner#1]
    #3817691 - 02/22/05 04:05 PM (19 years, 29 days ago)

Im gonna start from a culture. Thats the same as using tissue from a freshy right??


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??

Extras: Filter Print Post Top
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Agar & Peroxide Question [Re: oddiseus]
    #3817708 - 02/22/05 04:08 PM (19 years, 29 days ago)

yes, use 1.5 mL of peroxide, it's worked for me with agar wedges

Extras: Filter Print Post Top
Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 10 years, 4 months
Re: Agar & Peroxide Question [Re: Prisoner#1]
    #3817715 - 02/22/05 04:10 PM (19 years, 29 days ago)

should I just squirt it into the petri dish right after I pour the agar in?? and give a lil shakey shakey.?


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??

Extras: Filter Print Post Top
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Agar & Peroxide Question [Re: oddiseus]
    #3817740 - 02/22/05 04:15 PM (19 years, 29 days ago)

wait for your agar to cool to about 150F-160F and squirt it into the the agar,
give it a swirl to mix it in after each squirt and make sure you recap it first

Extras: Filter Print Post Top
Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 10 years, 4 months
Re: Agar & Peroxide Question [Re: Prisoner#1]
    #3817778 - 02/22/05 04:25 PM (19 years, 29 days ago)

Is their anyway you can eyeball the temp? I dont have anyway of measuring that. If I get a thermometer what kind should I get? Lab Grade?
Thanks for your help by the way..

And one other thing I can't believe you know who goatse boy is. That is hilarious.

-Odd


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags, Substrate Mix   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale


Similar ThreadsPosterViewsRepliesLast post
* Agar....Re-using question Erbaliza 1,279 8 12/02/02 06:26 AM
by rhizo
* Re: Agar w/ peroxide info Hippie3 1,617 2 05/07/00 02:20 AM
by mycofile
* Hydrogen Peroxide questions... djcontra 3,200 13 06/05/01 03:02 PM
by pl151
* Hydrogen Peroxide question.
( 1 2 all )
dumlovesyou 3,283 24 02/05/03 09:50 PM
by Hongosmeester
* Can I add certain % Peroxide to substrate mix? Numba9 8,384 5 08/23/01 09:31 AM
by shellacct
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,384 38 05/11/01 11:08 AM
by MAIA
* Ashtray's Questions and Answer Thread
( 1 2 3 4 ... 9 10 all )
Ashtray161 15,326 181 05/30/21 11:51 PM
by fahtster
* black pin mold on peroxidated MEYA YidakiMan 2,826 12 09/15/03 09:41 PM
by YidakiMan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,590 topic views. 17 members, 105 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.