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Invisibleagar
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Didn't pasturize, didn't sterilize & we will see.
    #3797303 - 02/18/05 06:21 AM (19 years, 1 month ago)

I'm posting these here, because they should all turn into a contam farm.

Which, I think they will, but maybe - just maybe ......not.

We will just have to wait & see, day by day & I will post update pic's.

If not, I discovered two (2) differing things. An Innovative cake & bulk substrate with optimal growth nutrients & a inventive method of preparing it.

They were knocked up the other day & have began to colonize cleanly.



UPDATE: (still going strong & no contam's)



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Edited by agar (02/24/05 05:15 AM)

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OfflineSunshinePounder
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Registered: 11/19/04
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3807736 - 02/20/05 05:15 PM (19 years, 1 month ago)

Very cool. :thumbup:


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My [url=www.http://tinyurl.com/6valr target=_blank]Unitarian Jihad Name[/url] is: Sister Claymore of The Short Path
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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: SunshinePounder]
    #3809359 - 02/21/05 12:46 AM (19 years, 1 month ago)

2/20 UPDATE...going stronger & no contams :thumbup:


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3809633 - 02/21/05 02:19 AM (19 years, 1 month ago)



This is looking so good, I decided to whip up a bigger batch to test run out. The white you see is condensation, not myc. These have not been knocked up - yet.


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Offlineshroommachine
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3812306 - 02/21/05 04:49 PM (19 years, 1 month ago)

whats in the jars?

Did you do multi-spore, karo, or grain?


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And I said, I don't care if they lay me off either, because I told, I told Bill that if they move my desk one more time, then, then I'm, I'm quitting, I'm going to quit. And, and I told Don too, because they've moved my desk ...four times already this year and I used to be over by the window and I could see the squirrels, and they were merry, but then, they switched from the Swingline to the Boston stapler, but I kept my Swingline stapler because it didn't bind up as much and I kept the staples for the Swingline stapler and its not okay because if they take my stapler then I'll set the building on fire.

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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: shroommachine]
    #3812807 - 02/21/05 06:15 PM (19 years, 1 month ago)

I have used all three (edit to add in individual containers), colonized WBS, Liquid Culture & MultiSpore in this test series.


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Edited by agar (02/21/05 06:29 PM)

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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3814916 - 02/22/05 12:29 AM (19 years, 1 month ago)

UPDATE 2/21
80% :thumbup: 80%:thumbup: 80%:thumbup:
Well, we are on the downhill side.


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Invisiblewoodzmun
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3815974 - 02/22/05 08:11 AM (19 years, 1 month ago)

All down hill now. :thumbup:

Edited by woodzmun (02/23/05 04:21 PM)

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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3820882 - 02/23/05 02:29 AM (19 years, 1 month ago)


About 90% colonized. Seems to be about 10% a day colonization rate.


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OfflineIGnosticAbhorI
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3824142 - 02/23/05 07:26 PM (19 years, 1 month ago)

Looking sweet, I was jus curious, what's multi-spore? I probably know what it is but not familar with the term. O and those jars look strange, i've noticed that Pf cakes and grain cakes look different. Or maybe it's jus my cakes, my pf's always looked like that whereas my wbs cakes were much of a brighter white and had rhizomorphs. Hmmm, just an observation, Gl, looking nice :thumbup:


-Gnostic

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InvisibleHolydiver
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3824158 - 02/23/05 07:29 PM (19 years, 1 month ago)

Quote:

IGnosticAbhorI said:
I was jus curious, what's multi-spore?
-Gnostic




A spore syringe would be multispore.


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OfflineIGnosticAbhorI
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: Holydiver]
    #3824715 - 02/23/05 09:19 PM (19 years, 1 month ago)

Hm, why is that considered multi-spore? Because it has millions and millions of spores? :smirk:

Well what exactly is an isolated strain? I thought that it had something to do with cloning and using agar...

-Gnostic

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OfflineIGnosticAbhorI
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3824724 - 02/23/05 09:20 PM (19 years, 1 month ago)

Sorry agar, don't mean to jack, just curious, wanna make sure that i'm able to follow what's going on :thumbup:

-Gnostic

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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3824955 - 02/23/05 10:06 PM (19 years, 1 month ago)

Isolate is healthy myc from agar plate (into spawn jar, or LC), or shroom tissue (clone).

MultiSpore is syringe load of spores, which have to germinate, mate & grow.

Isolate, or clone has done that already & is from prime growth or shroom.

Multispore is like gang-bang. Never know what the babies might be like, other than general strain charicteristics


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3825189 - 02/23/05 10:41 PM (19 years, 1 month ago)



Well.... we are about there & it took a week. Others are a few % behind. Going to give them a day or 3 more, to make sure all are at 110% colonization, then go from there.

Think I will shred one or 2 in a sanitized food processor & spawn into bulk of same substrate.

Others, I suppose I will fruit out as cakes.


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Offlinepenlight438094
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3825207 - 02/23/05 10:44 PM (19 years, 1 month ago)

so whats the sub, or is a mystery untill the flush


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: penlight438094]
    #3826105 - 02/24/05 02:59 AM (19 years, 1 month ago)

Hint - Cellulose, hemicellulose, lignin, fiber, carbohydrates, proteins, oils, lipids, polymers, vitamins, nitrogen, soluble macro mineral trace elements, and cultured (dead) cells of certain microbes, mixed into a holding material that has a high moisture & nutriant retention capacity, as well as good permeability to gas/air.

In other words, Sub will remain a mystery for awhile, as well as how it was pre-treated - prior to being innoc'ed.


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Edited by agar (02/24/05 05:26 AM)

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Invisiblenewuser1492
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3826725 - 02/24/05 09:49 AM (19 years, 1 month ago)

Sounds like some kind of protein bar from a health food store mixed with cotton.

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OfflineMAIA
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: newuser1492]
    #3827328 - 02/24/05 12:32 PM (19 years, 1 month ago)

Looking fine.
Good luck !

MAIA


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InvisibleNeedMoreSleep
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: MAIA]
    #3849464 - 02/28/05 10:08 PM (19 years, 1 month ago)

5 days since last update... did you birth them suckahs yet? make ur casings?  update please... lol :grin:

edit: sorry MAIA, was directed at agar :stoned:


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which we must fear most. And that is... the indifference of good men."
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Edited by NeedMoreSleep (02/28/05 10:09 PM)

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