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Magash
Da Bud Guru


Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Are ya board and have a few extra jars of brf around?
#3636374 - 01/16/05 08:10 PM (19 years, 4 months ago) |
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This tek is for newbs who have problems with grain or are too lazy (I was). This is also for people who only have one pf jars worth of mycelia. 1 jar can easily turn into 100 in 2 weeks time. This Pretty much is spawning with brf using Ziplocs.
I would highly suggest after 2-3 transfers renewing the mycelia from either multispore inoc or agar transfer to wbs or rye berry and then doing it again. Now that or just use brf with multispore.
materials 1. 1 colonized pf jar 2. 4 uncolonized but pc?ed sterile pf jars. 3. 1 Ziploc bag 4. 1 needle
procedure:
1. Clean your work area using alcohol and Lysol. Typically, wear a mask and gloves and sterilize the air with Lysol. Take note this tek requires no touching of the substrate! 2. Get 4 uncolonized jars and 1 colonized jar and turn them upside down and pat them until they are loose from the jar. 3. Open lids and dump off excess verm layer then dump 4 uncolonized cakes into Ziploc with colonized bag.( this needs to be done very quickly, less airtime the less chance for contams) 4. Now seal Ziploc and crunch up all cakes, making sure the colonized cake gets spread evenly throughout the bag. this way colonization takes place quickly. 5.LASTLY AND MOST IMPORTANTLY POKE HOLES USING A NEEDLE! use a small sewing needle so that a few holes are poked along the top of the Ziploc. and MAKE SURE your incubation room is very sterile. 6. let incubate for 3-6 days and full colonization occurs. no shaking necessary.
It is a simple newb way of multiplying spawn ten fold in 2 weeks. all you have to do is keep making brf jars.
There was an experiment conducted on some jars I heard about using the PF Tek and some with birdseed added to them. The ones using the PF-Birdseed tek produced much larger, and more potent mushrooms. Not to mention, they colonized almost twice as fast as the ones using regular pf tek. They were all cased with the same stuff.
Follow the regular PF Tek. The only difference is one part Pennington?s Wild Bird Feed (you can use other bird feeds that contain lots of white millet) was added in the mix. Prepare the bird seed as normal then add into the Brown Rice Flour/Vermiculite Mix and the water content needs to be adjusted accordingly. Once the water content is right (if you take a handful and squeeze it a few drops will fall) it was then placed in jars according to the regular PF tek and topped off with a 1/4" layer of dry vermiculite (to help keep out airborne contams that come through the inoculation points). The jars were then pressure cooked for 1 hour and then sat in the Pressure cooker overnight to cool. Once cool, they were inoculated as usual.
The jars that used the PF-Birdseed Tek performed much better, and produced much larger/potent mushrooms than the ones using the regular PF-Tek.
-------------------- All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
Join us at the Growery!
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JaRRn
Lost in Space


Registered: 05/20/04
Posts: 1,155
Loc: Standing on the Cosmic Sh...
Last seen: 2 years, 16 days
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Re: Are ya board and have a few extra jars of brf around? [Re: Magash]
#3636396 - 01/16/05 08:16 PM (19 years, 4 months ago) |
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Another A1 Post. Cheers! Im going to try this. Thanks.
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CaRnAgECaNdY
Tool's groupie


Registered: 04/09/04
Posts: 11,505
Loc: Billy Howerdel's closet
Last seen: 10 months, 10 days
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Re: Are ya board and have a few extra jars of brf around? [Re: JaRRn]
#3636453 - 01/16/05 08:29 PM (19 years, 4 months ago) |
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Right on! I just may use this, Thanks!
--------------------
The secret to being funny is to say smart things stupidly, or is it stupid things smartly? Whatever..it's not rocket surgery...or something like that.
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 11 months, 23 days
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Re: Are ya board and have a few extra jars of brf around? [Re: CaRnAgECaNdY]
#3637281 - 01/16/05 11:17 PM (19 years, 4 months ago) |
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Awesome, magash, once again your laziness proves to help us all out =)
Well me and a foaf did this with Poo. He had like 4 jars of corn. We did pretty much what you just did with brf cakes.
Thankx once AGAIN for your contribution...
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Ir0NLunG68420
BLaZeDuP


Registered: 11/27/04
Posts: 422
Loc: Tripville
Last seen: 3 years, 2 months
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Re: Are ya board and have a few extra jars of brf around? [Re: IGnosticAbhorI]
#4062427 - 04/16/05 01:02 PM (19 years, 1 month ago) |
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This post should be archived because it works like a dream. Magash is a lazy pimp! Thanks. The BRF to WBSF (Wild Bird Seed Flour) tek is working great(non-colonized and colonized cakes in a bag). Started them on 4-15-05 that is what these pictures are of today 4-18-05.
 
-------------------- "I never meant to give you mushrooms girl, I never meant to bring you to my world, but now you're sitting in the corner crying, and now it's MY FAULT, MY FAULT." -Eminem- Second grow log GT & B+ First grow log Mexicub & B+
Edited by Ir0NLunG68420 (04/18/05 01:45 PM)
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KaptKid
Spaced Pirate


Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 3 months
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Re: Are ya board and have a few extra jars of brf around? [Re: Ir0NLunG68420]
#4062509 - 04/16/05 01:37 PM (19 years, 1 month ago) |
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Far Out. Will have to give this one a try.Archive this shit.
-------------------- Child of the 60's, Tripping ever since.
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Shiin
Orthodox Christian

Registered: 11/14/04
Posts: 129
Last seen: 8 years, 6 months
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Re: Are ya board and have a few extra jars of brf around? [Re: KaptKid]
#4062527 - 04/16/05 01:43 PM (19 years, 1 month ago) |
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Nice stuff magash, im def gunna try this thanks a million
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Respect............... Everything I state is strickly false information. "I do not take drugs, I am drugs" Salvador Dali God destroyeth the wisdom of the wise, by the foolishness of preaching, and calleth not the wise, mighty, and noble, but the foolish, weak, and men of no account. 1 Corinthians 1:27
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pleco
Astralnaut

Registered: 03/08/05
Posts: 203
Last seen: 16 years, 10 months
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Re: Are ya board and have a few extra jars of brf around? [Re: Shiin]
#4062678 - 04/16/05 02:42 PM (19 years, 1 month ago) |
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Nice =] Do you think it'll it work out with 3-4 half colonized jars?
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goldtop57
Stranger

Registered: 04/10/05
Posts: 86
Loc: England
Last seen: 18 years, 6 months
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Re: Are ya board and have a few extra jars of brf around? [Re: pleco]
#4062890 - 04/16/05 04:00 PM (19 years, 1 month ago) |
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Your a smart motherfucker. I'm trying it tonight with WBS and an ovenbag. The substrate is warming in the oven as we speak. (I PC'd the WBS earlier today)
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4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 10 years, 12 days
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Re: Are ya board and have a few extra jars of brf around? [Re: goldtop57]
#4062988 - 04/16/05 04:57 PM (19 years, 1 month ago) |
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The question is can u just boil the PF jar with the little bit of birdseed in it.
and what are the specific ratios you want? do you do total volume divided by 5 and do 2 parts verm, 1 part flour, 1 part water and 1 part birdseed?
-------------------- all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say. _______ "Trust me, I'm from the future. Actually I'm not from the future, but I did lived there for a while." - Baby Hitler
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MycoJunkie
Psilanthropist

Registered: 11/04/04
Posts: 963
Loc: .4merica
Last seen: 18 years, 4 months
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Re: Are ya board and have a few extra jars of brf around? [Re: 4hodmt]
#4063067 - 04/16/05 05:19 PM (19 years, 1 month ago) |
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Quote:
4hodmt said: The question is can u just boil the PF jar with the little bit of birdseed in it.
and what are the specific ratios you want? do you do total volume divided by 5 and do 2 parts verm, 1 part flour, 1 part water and 1 part birdseed?
Good questions.
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mattymonkey
Feel Like aStranger...


Registered: 11/07/04
Posts: 973
Last seen: 11 years, 4 months
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Re: Are ya board and have a few extra jars of brf around? [Re: MycoJunkie]
#4063177 - 04/16/05 06:13 PM (19 years, 1 month ago) |
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btw.. and im sure magash ment this, but you want to use a brand new ziplock baggy...
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Ir0NLunG68420
BLaZeDuP


Registered: 11/27/04
Posts: 422
Loc: Tripville
Last seen: 3 years, 2 months
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Re: Are ya board and have a few extra jars of brf around? [Re: mattymonkey]
#4068808 - 04/18/05 01:48 PM (19 years, 1 month ago) |
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*Bump
Added pictures of this tek to my first post above.
-------------------- "I never meant to give you mushrooms girl, I never meant to bring you to my world, but now you're sitting in the corner crying, and now it's MY FAULT, MY FAULT." -Eminem- Second grow log GT & B+ First grow log Mexicub & B+
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Dr_Demented
Shape Shifter

Registered: 10/23/04
Posts: 144
Loc: At the other end
Last seen: 17 years, 30 days
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Re: Are ya board and have a few extra jars of brf around? [Re: Ir0NLunG68420]
#4068852 - 04/18/05 02:15 PM (19 years, 1 month ago) |
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Also an idea would be to transfer all that mixed substrate from zippy to an filter patch bag witch is then sealed with an impulse sealer. Thus giving gas exchange and not having to have an super sterile incubator... Just an idea.
I will absolutely try this! Instead of inoculating 12 jars I could just do 4 or maybe even 3...
Time saver! Syringe saver!
/Dr.
-------------------- -It all makes sence if you think about it for a while. All you need to success in growing!
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tracerz
edible eater
Registered: 05/08/05
Posts: 17
Loc: wa forest
Last seen: 18 years, 1 month
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Re: Are ya board and have a few extra jars of brf around? [Re: Dr_Demented]
#4181189 - 05/16/05 02:59 AM (19 years, 8 days ago) |
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my friend got bacteria allot, with a tit with a few air holes room air Maybe you could super glue a filter on and micro the baggie, with or with out the pre sterile brf/wbs mix, Or just keep your tit in a filtered closet maybe but i don't see how this would work when you go in and out. i guess a room or big walk in closet would be best to filter.
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 11 months, 23 days
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Re: Are ya board and have a few extra jars of brf around? [Re: 4hodmt]
#4181417 - 05/16/05 07:13 AM (19 years, 8 days ago) |
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Quote:
4hodmt said: The question is can u just boil the PF jar with the little bit of birdseed in it.
and what are the specific ratios you want? do you do total volume divided by 5 and do 2 parts verm, 1 part flour, 1 part water and 1 part birdseed?
The more WBS the more Nutes, you decide...
And Grains have Endospores and Must be Pressure Cooked to kill them (after you let the endospores germ. of course)...
-Gnostic
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willmafingerdo
the noob


Registered: 02/08/05
Posts: 1,103
Last seen: 3 months, 2 days
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Re: Are ya board and have a few extra jars of brf around? [Re: tracerz]
#4181433 - 05/16/05 07:26 AM (19 years, 8 days ago) |
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you could just get some first aid tape and put over the holes or use some of the round bandaids over the holes for a quick filter.. ive been using first aid tape over my popcorn jars and kakes and havent had any problems yet..
--------------------
I am not lost.. i am going there looking for it.. and when i get there and find it, i will know what it is.. "The way to stop violence is not to go out and let your head be beaten in but to say, you want to take my life risk yours!" "are you sure u want to eat all them shrooms??" "yes i want to hear what my mind has to say..." remember opions are like assholes, everyone has one and they all stink.
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ClosetMonster
No Fear


Registered: 10/13/14
Posts: 238
Last seen: 9 years, 3 months
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Re: Are ya board and have a few extra jars of brf around? [Re: Magash]
#21112839 - 01/13/15 10:25 AM (9 years, 4 months ago) |
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um so what do you do with the bag once its fully done ?
where do you dump it to?
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Are ya board and have a few extra jars of brf around? [Re: ClosetMonster]
#21112856 - 01/13/15 10:31 AM (9 years, 4 months ago) |
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Little late to the party chief
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ClosetMonster
No Fear


Registered: 10/13/14
Posts: 238
Last seen: 9 years, 3 months
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Re: Are ya board and have a few extra jars of brf around? [Re: PirateSwazey]
#21112875 - 01/13/15 10:37 AM (9 years, 4 months ago) |
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Quote:
PirateSwazey said: Little late to the party chief 
I like to time travel
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