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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Offlineherby
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Registered: 07/25/04
Posts: 69
Last seen: 19 years, 3 months
reusing jars
    #3438664 - 12/02/04 04:48 PM (19 years, 4 months ago)

I think my spores died before mycelium could start growing due to a high incubation temperature. Would i be able to reuse the
substrate in the jars and resterilize them or do i have to start from the beginning?

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OfflineJacquesCousteau
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Registered: 06/10/03
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Re: reusing jars [Re: herby]
    #3438683 - 12/02/04 04:52 PM (19 years, 4 months ago)

How long has it been since inoculation? And what temperature did it get up to? You might be jumping the gun and might not even have to do anything but wait longer...

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Offlineherby
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Posts: 69
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Re: reusing jars [Re: JacquesCousteau]
    #3438724 - 12/02/04 04:59 PM (19 years, 4 months ago)

5 days ago and the temp was hot!! like really hot!! i was just cool enough to handle

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Offlinelesstutrey
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Re: reusing jars [Re: herby]
    #3438746 - 12/02/04 05:01 PM (19 years, 4 months ago)

hot? whats hot? 90? 100?

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OfflineJacquesCousteau
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Re: reusing jars [Re: herby]
    #3438934 - 12/02/04 05:39 PM (19 years, 4 months ago)

I would give it a few more days to be sure it's dead.

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Offlinedjred
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Registered: 08/27/04
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Re: reusing jars [Re: JacquesCousteau]
    #3439019 - 12/02/04 05:58 PM (19 years, 4 months ago)

yeah my jars of karo jumped up above 90 mid + so im sure i killed my spores. been over 2 weeks no growth so i gave up on them..

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InvisiblePrisoner#1
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Re: reusing jars [Re: herby]
    #3439143 - 12/02/04 06:20 PM (19 years, 4 months ago)

spores are pretty resilient, they can take temps over 105F without blinking an eye,
I gave a friend a dozen syringes that were stored in an outside shed for a couple
of years and the temps in there reached at least that through the hotter parts
of july and august, he's had no problem with them germinating...


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Offlinehyphae
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Re: reusing jars [Re: Prisoner#1]
    #3439466 - 12/02/04 07:31 PM (19 years, 4 months ago)

Myc can handle temps up to 106F. and survive and spores can handle 122F. maybe slightly higher so don't sweat it. Now if it felt warm (98.6+) or very warm (122-160F.) then you could have definitely fried your spores so next time remember in this hobby patience is a virtue! :wink: Better luck next time bro. :thumbup:


--------------------
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Offlinedjred
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Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
Re: reusing jars [Re: hyphae]
    #3439546 - 12/02/04 07:41 PM (19 years, 4 months ago)

oh i did have some growth..no the jars didn't get above 90+ so no worries ...well now i have them just siting on a dresser hanging out. so i might have a chance on my karo jars ,but 2 weeks seems kinda long.. well fuck it i still don't care its only time and effort..

well i dunno know for sure about the temps didn't have atemp gauge yet but my wbs seed jars are doing 86 easy hanging out in a tnt

Edited by djred (12/02/04 07:43 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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