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OfflineAnonymousNewbie
Mad Scientist
Registered: 06/20/04
Posts: 211
Loc: England
Last seen: 18 years, 9 months
Check out these syringes! (Pictures)
    #3348908 - 11/11/04 09:07 PM (19 years, 4 months ago)

There are huge clumps of what i assume is spores in these syringes i ordered from the lil guy. I was concerned that when i inoculate, these clumps might not come out? Will they come out - or should i shake them up a lil bit?
The picture isn't that great - but you can make out the Clumps of spores on the top 3 syringes.

Edited by Magash (11/12/04 12:24 PM)

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Offlineazurescens
member
Registered: 02/17/04
Posts: 717
Last seen: 15 years, 9 months
Re: Check out these syringes! (Pictures) [Re: AnonymousNewbie]
    #3348920 - 11/11/04 09:09 PM (19 years, 4 months ago)

shake em up my friend...shake em good

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OfflineCornelius
Heinous Anus
Male User Gallery

Registered: 02/12/04
Posts: 694
Loc: T.O.
Last seen: 9 years, 11 months
Re: Check out these syringes! (Pictures) [Re: AnonymousNewbie]
    #3348921 - 11/11/04 09:09 PM (19 years, 4 months ago)

shake them up really well before innoculating - don't worry too much if they don't comeout, cause there's lot's of spores elsewhere in the water...


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Even as the nineteenth century had to come to terms with the notion of human decent from apes, we must now come to terms with the fact that those apes were stoned apes. Being stoned seems to have been our unique characteristic.

- Terence McKenna



1946 - 2000

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Offlinejuende
feministpresence

Registered: 02/20/04
Posts: 729
Last seen: 9 years, 1 month
Re: Check out these syringes! (Pictures) [Re: AnonymousNewbie]
    #3348922 - 11/11/04 09:09 PM (19 years, 4 months ago)

shake them up[cap on of course] and good luck!

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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 9 years, 10 months
Re: Check out these syringes! (Pictures) [Re: AnonymousNewbie]
    #3348924 - 11/11/04 09:10 PM (19 years, 4 months ago)

breka up those spores.
simply:

light lighter, insert tip of needle into heart of flame, slowly draw back air through the flame into the needle...this makes it sterile.


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all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

- Baby Hitler

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Invisibleblissedout
Male User Gallery

Registered: 11/11/04
Posts: 22,320
Loc: Yonder Flag
Re: Check out these syringes! (Pictures) [Re: azurescens]
    #3348935 - 11/11/04 09:13 PM (19 years, 4 months ago)

shake those bad boys up to disperse the spores more evenly
peace


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:murray:

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Offline13eetleJuice
the ghost with the most
Male

Folding@home Statistics
Registered: 10/28/04
Posts: 2,253
Loc: 6' under pushin up shroom...
Last seen: 5 years, 4 months
Re: Check out these syringes! (Pictures) [Re: 4hodmt]
    #3348945 - 11/11/04 09:16 PM (19 years, 4 months ago)

It's a good thing. Spores naturally try to clump together in the suspension solution. And since spores are microscopic, if you see something you are actually seeing thousands of spores. Just shake VIGOROUSLY to break up some of the spores from the clump. Oh and props to www.thelittleguy.com for the potent syringes. TLG rocks!


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OfflineAnonymousNewbie
Mad Scientist
Registered: 06/20/04
Posts: 211
Loc: England
Last seen: 18 years, 9 months
Re: Check out these syringes! (Pictures) [Re: blissedout]
    #3348949 - 11/11/04 09:16 PM (19 years, 4 months ago)

Thanks for the advice everyone - My buddy isn't going to sterilize the needle that way though 4hodmt. He was told that wiping the needle with alcohol before injection was a good enough way to do it. He is also a recovering pyromaniac, so he has to keep his distance from lighters. heehee... j/k


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Don't grow mushrooms if it is illegal where you live.

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Offline13eetleJuice
the ghost with the most
Male

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Registered: 10/28/04
Posts: 2,253
Loc: 6' under pushin up shroom...
Last seen: 5 years, 4 months
Re: Check out these syringes! (Pictures) [Re: AnonymousNewbie]
    #3348980 - 11/11/04 09:22 PM (19 years, 4 months ago)

4hodmt was talking about sterilizing the "air" you pull back into the syringe body by sticking the needle in a flame while pulling back the plunger. That way you don't inadvertantly pull any bacteria into the syringe. It's best to use an alcohol flame or a butane lighter for this. As a regular bic lighter sometimes leaves behind that black residue.


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OfflineJAMIEK
member
Registered: 07/14/04
Posts: 174
Last seen: 14 years, 6 months
Re: Check out these syringes! (Pictures) [Re: blissedout]
    #3348992 - 11/11/04 09:24 PM (19 years, 4 months ago)

ditto

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OfflineAnonymousNewbie
Mad Scientist
Registered: 06/20/04
Posts: 211
Loc: England
Last seen: 18 years, 9 months
Re: Check out these syringes! (Pictures) [Re: 13eetleJuice]
    #3348995 - 11/11/04 09:25 PM (19 years, 4 months ago)

Why would i need to pull air into the syringe? I'm pretty sure there is enough air in those syringes for a good shaking. Check out that air bubble. Thanks anyway though - sorry i misunderstood 4hodmt.


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Don't grow mushrooms if it is illegal where you live.

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OfflineAnonymousNewbie
Mad Scientist
Registered: 06/20/04
Posts: 211
Loc: England
Last seen: 18 years, 9 months
Re: Check out these syringes! (Pictures) [Re: AnonymousNewbie]
    #3349024 - 11/11/04 09:31 PM (19 years, 4 months ago)

Is my buddy waisting his hard earned pimpin hoes money by buying syringes every time. They aren't that bad economically, and he feels safe from contams. - but just how easy is all this making your own syringes non-sence? If someone could explain to me the cost of making 5 or so syringes, and the (relative) simplicity of the process - that person might be saving a hoe or two a slapping this week.


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Don't grow mushrooms if it is illegal where you live.

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Offlineonetime
onetime
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Registered: 11/13/03
Posts: 3,609
Last seen: 13 years, 3 months
Re: Check out these syringes! (Pictures) [Re: AnonymousNewbie]
    #3349044 - 11/11/04 09:34 PM (19 years, 4 months ago)

yeah shake them good once i had a of pf brand syringe a few yrs ago right brfor he went out of beussness it was sooo dark it had brown stringy chunks in it and they cloged it and i had on jar get like 4-6cc's becouse it all shot out at once


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See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want

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Offline13eetleJuice
the ghost with the most
Male

Folding@home Statistics
Registered: 10/28/04
Posts: 2,253
Loc: 6' under pushin up shroom...
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Re: Check out these syringes! (Pictures) [Re: onetime]
    #3349073 - 11/11/04 09:39 PM (19 years, 4 months ago)

check out this tek for making syringes from prints. Quite simple yet effective. http://www.shroomery.org/index/par/7942


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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: Check out these syringes! (Pictures) [Re: 13eetleJuice]
    #3349571 - 11/11/04 11:29 PM (19 years, 4 months ago)

shake, but think about what you are saying, though...

If it went it through the needles, they can come out through the needles. :wink:

Sam


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Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.

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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Check out these syringes! (Pictures) [Re: 13eetleJuice]
    #3350291 - 11/12/04 05:31 AM (19 years, 4 months ago)

Quote:

13eetleJuice said:
4hodmt was talking about sterilizing the "air" you pull back into the syringe body by sticking the needle in a flame while pulling back the plunger. That way you don't inadvertantly pull any bacteria into the syringe. It's best to use an alcohol flame or a butane lighter for this. As a regular bic lighter sometimes leaves behind that black residue.


lol, a regular bic lighter is a butane lighter.

I use a propane torch. Just set it to an ultra-low setting. No residue, and a canister should last forever(if just doing syringe needles).

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OfflineDMTelepath
Nut

Registered: 10/29/04
Posts: 567
Loc: States of America
Last seen: 19 years, 1 month
Re: Check out these syringes! (Pictures) [Re: discman1]
    #3350329 - 11/12/04 06:01 AM (19 years, 4 months ago)

Is shaking the syringe before innoculating very important? Yesterday when injecting 5 quart jars with spores, i didn't shake the syringe. This grow is sort of an experiment for me, and i wouldn't be suprised if all 5 jars get contams and are failures (even though i took many steps to assure sterility). Peace


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Me, Myself, and GOD

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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: Check out these syringes! (Pictures) [Re: DMTelepath]
    #3350340 - 11/12/04 06:07 AM (19 years, 4 months ago)

It's very important for PF jars, because you only use 1cc per jar and only .25cc per site. For quart jars where you put 1-2cc in one place, you have a better chance. This is a common rookie and veteran error. The consequences can seem more severe on newbies because that's usually all they have. But it'll probably germinate for you, though. Good luck!

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.

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OfflineDMTelepath
Nut

Registered: 10/29/04
Posts: 567
Loc: States of America
Last seen: 19 years, 1 month
Re: Check out these syringes! (Pictures) [Re: Sam1912]
    #3350347 - 11/12/04 06:11 AM (19 years, 4 months ago)

I put about 1.3-1.4cc per quart size jar. Geez i really should read up some more before i attempt a real grow, i bet 6 months ago i was way more informed and retained a lot more mushroom growing knowledge. Ah well, if i wouldn't have used up my sterilized jars, they would have rotted and been thrown in the trash. Best thing i could have done...invitro growing.


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Me, Myself, and GOD

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Offlinecall_me_kido
Philosopher
 User Gallery

Registered: 10/26/04
Posts: 354
Loc: In your dreams
Last seen: 14 years, 3 months
Re: Check out these syringes! (Pictures) [Re: DMTelepath]
    #3350461 - 11/12/04 07:12 AM (19 years, 4 months ago)

Id say making liquid myc culture syringes with agar is better then making spore syringes, magash had a great tek on it. . .


Kido


--------------------
"If we knew what it was we were doing, it would not be called research, would it?"
- Albert Einstein (1875-1955)

"A is A" -Aristotle

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