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Offlinejethro
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Registered: 09/19/04
Posts: 43
Last seen: 18 years, 7 months
Producing Mycellial Syringes.....PMS
    #3154291 - 09/19/04 09:59 PM (19 years, 6 months ago)

What method of making mycelium syringes work the best for you?


-shooting spores into honey water and then sucking up mycellial growth

-taking a tissue culture from the inner meat of the mushroom

-growing cultures on agar and blending to make syringes


Which one produces the least contams.


My friend wants to do mycobags, but until now grain to grain was working fine. Every time he has tried honey water or tissue he has gotten contams. Is H2O2 needed?


Is a contam-free mycellial syringe possible

Edited by jethro (09/19/04 10:01 PM)

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Invisiblemuse_sick
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Registered: 12/15/03
Posts: 9,399
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Re: Producing Mycellial Syringes.....PMS [Re: jethro]
    #3154820 - 09/20/04 12:23 AM (19 years, 6 months ago)

IMO the best innovation for the home cultivator is the karo jars
just 100 mL h20/1 tablespoon karo
PC for 10 minutes
let cool,
shoot up with 2ccs of multispore or myc
let colonize
break up myc with a sterile syringe by sucking in and shooting out water
then fill syringe
shoot up substrate
colonization time is drastically cut
h202 is not needed but if you find it helps then i encourage you to experiment at low dilutions
h202 is not needed


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Offlinediscman1
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Registered: 08/24/04
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Last seen: 19 years, 3 months
Re: Producing Mycellial Syringes.....PMS [Re: muse_sick]
    #3154859 - 09/20/04 12:41 AM (19 years, 6 months ago)

Pardon my n00bage, but what is karo?

Links welcomed. :wink:

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Invisiblemuse_sick
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Re: Producing Mycellial Syringes.....PMS [Re: discman1]
    #3154888 - 09/20/04 12:48 AM (19 years, 6 months ago)

karo is light corn syrup
available in any supermarket


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Offlinediscman1
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Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Producing Mycellial Syringes.....PMS [Re: muse_sick]
    #3154955 - 09/20/04 01:16 AM (19 years, 6 months ago)

Ooh, duh. :wink: Interesting. So can you just innoculate the bottle as it comes, since you would assume that it is sterile?

I think I'm in need of a tek, here. I'm interested in this avenue.

Edit: On second look, you do say that you need to PC them. Ok.

As for the H2O2... I suppose it certainly doesen't hurt anything. H2O2 degrades rapidly into H2O and O when heated, so I guess you'll have to be the judge of whether it's worth it or not.

It might be better to add the H2O2 after being PC'd. It would help oxygenate the corn syrup. Adds contam risk, but it might speed up mycelium growth for a few days.

I'm under the assumption that the mycelium grows rather slowly in such liquid substrates. My guess would be due to lack of gas exchange.

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Invisiblemuse_sick
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Re: Producing Mycellial Syringes.....PMS [Re: discman1]
    #3155015 - 09/20/04 01:47 AM (19 years, 6 months ago)

no it grows fairly fast
5 days you will have a usable amount of myc
if you incubate


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Offlineonetime
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Registered: 11/13/03
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Re: Producing Mycellial Syringes.....PMS [Re: muse_sick]
    #3155043 - 09/20/04 09:07 AM (19 years, 6 months ago)

well one could also scrape spores in to a shot glass and then suck up stairal honey or kero and squirt that into the shot glass and suck it up again and incubate the sryinge and then inject couldnt you ?


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Offlinejethro
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Registered: 09/19/04
Posts: 43
Last seen: 18 years, 7 months
Re: Producing Mycellial Syringes.....PMS [Re: onetime]
    #3155144 - 09/20/04 09:59 AM (19 years, 6 months ago)

I am familiar on how to make syringes, but they always contam. Even with H2O2.

we are talking like ten tries with zero success.

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InvisibleMagash
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Re: Producing Mycellial Syringes.....PMS [Re: jethro]
    #3155164 - 09/20/04 10:06 AM (19 years, 6 months ago)

Ok, here is what I did and it's in the faq as the poorman's syringe tek. (it should be the lazy man's tek).

Stick syringes with steril water in em in a glove box. Put in a shotglass and a fully colonized cake.

Well if you know the cake is fully colonized you know it's not contamed. I just shoot the water into the shotglass and pull the plunger out of the syringe. Scrape a little myc off of the cake and put it in the syringe by hand. Put the plunger back in suck up the water and shake like a spaztic monkey before using them. This was tested on over 100 cakes by two different people.  :grin: :thumbup: :wink: :cool:


--------------------
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Offlinejethro
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Registered: 09/19/04
Posts: 43
Last seen: 18 years, 7 months
Re: Producing Mycellial Syringes.....PMS [Re: Magash]
    #3156759 - 09/20/04 04:28 PM (19 years, 6 months ago)

Thank you, I definately need something that works. Don't wanna waste those bags or all that grain.

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