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Offlinetylerisafunguy
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Registered: 09/06/23
Posts: 2
Last seen: 3 months, 18 days
Identidying Contamination On My Agar Plates (Condensations, polyfill, micropore tape)
    #28634569 - 01/25/24 02:18 PM (3 months, 21 days ago)

Hello everyone! This is my first post on Shroomery. I have been growing mushrooms for 2 years now. I am wanting your feedback on these 3 agar plates. I think the agar plate labeled "purple Mystic" with a number three on it is the only one without contamination. My friend, who used to grow mushrooms, told me that you don't want any condensation, which can be seen on the other two agar plates. If it has condensation, that normally means it's contaminated. Is that true? Any other helpful tips and tricks for identifying contamination would be great.

Also, I added the mushroom spores around December 7, 2023, and waited two weeks but didn't see any growth whatsoever. I forgot about these plates until I discovered substantial growth yesterday. I thought it normally only takes about two weeks to see growth, and I'm wondering if it has to do with the polyfill that I used to seal the agar plates. I thought polyfill has pores in it so that there can be gas exchange. Also, is gas exchange even necessary when spores are on an agar plate? Also, if you see on plate labeled number three I put micropore tape on it yesterday.

Thank you so much for taking the time to answer my questions



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OfflineScrewup
Googles your dumb questions
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Registered: 01/27/22
Posts: 6,340
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Re: Identidying Contamination On My Agar Plates (Condensations, polyfill, micropore tape) [Re: tylerisafunguy]
    #28635191 - 01/26/24 03:50 AM (3 months, 20 days ago)

Bump for you cause I’m too lazy to help


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Offline3.A.M
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Registered: 10/17/22
Posts: 1,240
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Re: Identidying Contamination On My Agar Plates (Condensations, polyfill, micropore tape) [Re: Screwup] * 1
    #28635219 - 01/26/24 04:40 AM (3 months, 20 days ago)

:
Quote:

Screwup said:
Bump for you cause I’m too lazy to help



:rofl:

Condensation on grain jars can potentially mean contamination but not on agar, simple environmental temp changes can cause that, don’t use poly fill for plates, there should be enough oxygen in a plate to see you through plenty of growth when sealed,  as to where and if to transfer? Sometimes it’s not obvious but fast, consistent, clean growth is always desirable, if you’re uncertain you can always transfer an ideal candidate to water agar or brf puck to clean it up and be certain, which I would definitely do with what you have there.
Also, micropore is a bitch (been there, done that) try either cling film cut down to size or even better, grafting tape πŸ‘


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Offlinetylerisafunguy
Stranger
Registered: 09/06/23
Posts: 2
Last seen: 3 months, 18 days
Re: Identidying Contamination On My Agar Plates (Condensations, polyfill, micropore tape) [Re: 3.A.M]
    #28635509 - 01/26/24 11:07 AM (3 months, 20 days ago)

Thank you so much! I really appreciate your help

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