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Offlinecybork
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How to do needle sterilisation: from tip to end or the other way around?
    #28624185 - 01/17/24 04:19 AM (3 months, 29 days ago)

I have a question about needles. Quite sure this is a duplicate question. Sorry for that in advance.

When flame sterilising the needle for an already partly used (and still attached) syringe, do you go from the tip to the end, or from the end to the tip?

I go from end to tip, but I just wonder what is considered as best practice?

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OnlineAspectOfTheCreator
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Re: How to do needle sterilisation: from tip to end or the other way around? [Re: cybork] * 1
    #28624187 - 01/17/24 04:27 AM (3 months, 29 days ago)

It doesnt matter. The needle isnt long enough to have only one end hot enough. If any of it is glowing red then the whole thing is hot enough. Still, flame the whole thing.


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Edited by AspectOfTheCreator (01/17/24 05:26 AM)

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Offlinecybork
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Re: How to do needle sterilisation: from tip to end or the other way around? [Re: AspectOfTheCreator] * 1
    #28624242 - 01/17/24 06:53 AM (3 months, 29 days ago)

Ah yes, that sounds logical. Thanks!

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OfflineBlufuut180
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Re: How to do needle sterilisation: from tip to end or the other way around? [Re: cybork] * 3
    #28656873 - 02/12/24 01:57 PM (3 months, 3 days ago)

Be careful of overdoing it and melting the plastic where it mounts to the luer lock. ask me how I know lol.

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OfflineLogicaL ChaosM
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Re: How to do needle sterilization: from tip to end or the other way around? [Re: cybork]
    #28657769 - 02/12/24 11:00 PM (3 months, 2 days ago)

As Aspect said, it doesnt matter, just flame the whole tip.

I was considering getting new, unused tips to use right before inoculation as they are already sterile in the package.


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Offlinecybork
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Re: How to do needle sterilization: from tip to end or the other way around? [Re: LogicaL Chaos] * 1
    #28658295 - 02/13/24 11:20 AM (3 months, 2 days ago)

Yeah light it up!

My next set of syringes and needles will be seperate ones. Currently I have sets where both are packed together, so I don't have sterile needles on hand. My next purchase for those will be seperate ones, plus things to close the syringe. Thanks for the suggestion though!

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OfflineAndImTheHighOne
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Re: How to do needle sterilization: from tip to end or the other way around? [Re: cybork] * 2
    #28660473 - 02/15/24 07:58 AM (3 months, 12 hours ago)

One thing to note about this: if you're inoculating a PF jar, granules of brown rice flour have a tendency to get stuck in the syringe needle. Flaming obviously vaporizes the water present in the needle. If you start at the base of the needle, you will notice those granules are shot out of the tip from the pressure created by the steam. Flaming the tip first will sometimes cause those granules to be forced back into needle clogging it. Just something I have noticed about PF Tek.


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Invisiblebarfolomew
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Re: How to do needle sterilization: from tip to end or the other way around? [Re: AndImTheHighOne]
    #28662111 - 02/16/24 11:52 AM (2 months, 30 days ago)

For thick LC, you can also get stainless steel meat injectors online or stores like Walmart that you can seal in autoclave bags or myco bag and PC, then remove in front of laminar flowhood or with SAB + flame sterilization. Not for self sealing injection ports, but with proper FH or SAB technique sterile spawn jars can be opened and inoculated with minimal risk of contamination. 

https://www.walmart.com/ip/Heavy-Duty-304-Stainless-Steel-Meat-Injector-Kit-with-60ml-Large-Capacity-Barrel-with-3-Commercial-Marinade-Needles/476283928


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