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InvisibleBlimeyGrimey
Collector of Spores
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Registered: 08/24/05
Posts: 3,799
Loc: Puget Sound
15 year old Psilocybe stuntzii spore microscopy * 3
    #28616378 - 01/10/24 12:50 PM (4 months, 5 days ago)

I've recently been trying to revive some of my spore collection. The spores range from 17 years old to 8 years old.

I've had no success with germination of the 15 year old stuntzii spores and I'm down to my last two F1 print from my 2008/2009 indoor grow.

I decided to check them out under a scope now that I have received my new scope.

Here are what they look like after 15 years of storage on foil, stored in a zip lock type baggie at room temp.

Microscopy done at 1000x (25x by 40x) * waiting on my Cargille Oil to arrive to use the 100x objective







I was expecting them all to look like the one that is ruptured,  but was surprised to see that most look completely normal.

These spores were hydrated for 3 weeks in a solution of distilled water with 1.8mg of disodium adenosine triphoshate per ml with a tiny bit of jet-dry.

Scope used is a WJTQTBT W-120, which is a rebranded Amscope B-120.


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Invisiblethe_chosen_one
On the Darkslide
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Registered: 09/11/06
Posts: 3,250
Loc: 1984
Trusted Cultivator
OG Cultivator
Re: 15 year old Psilocybe stuntzii spore microscopy [Re: BlimeyGrimey]
    #28616502 - 01/10/24 02:57 PM (4 months, 5 days ago)

They look better than I do after 15 years. :lol: Cool to see you using the ATP! I've been playing with it a little myself.


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"Luck favors the observant." - Workman


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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,799
Loc: Puget Sound
Re: 15 year old Psilocybe stuntzii spore microscopy [Re: the_chosen_one]
    #28616603 - 01/10/24 04:34 PM (4 months, 5 days ago)

Quote:

the_chosen_one said:
They look better than I do after 15 years. :lol: Cool to see you using the ATP! I've been playing with it a little myself.




Same here, I've definitely had way less gray hairs when these spores were fresh.

Have you noticed any benefits to using the ATP in any way? I have 2.3 grams left and trying to come up with some good uses for it.

Does the ATP withstand sterilization temps?

I've been making solutions with distilled water and running it through a 0.22um wheel filter into a sterile glass vial. So far I haven't run into any contamination issues using filter sterilization, as long as I start with very clean starting materials.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

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Invisiblethe_chosen_one
On the Darkslide
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Registered: 09/11/06
Posts: 3,250
Loc: 1984
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Re: 15 year old Psilocybe stuntzii spore microscopy [Re: BlimeyGrimey]
    #28616747 - 01/10/24 06:46 PM (4 months, 5 days ago)

It binds to agarose beads and holds up to the standard 15 minutes. I've had mixed results. About a little over half have been very promising. It's very easy to use too much and cause an inhibitive environment though. Sounds like we're using about the same percentage per ml. I was going to reduce just slightly on my next round. What little I've done has been strictly with exotics. Mostly Zapotecorum but my most recent possible success was bringing Caerulescens Tonila out of a long sleep. I've had to rework it a bit but it's coming back strong now. :thumbup:


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OfflinePscientist
KushKaptain
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Registered: 11/13/09
Posts: 2,687
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Last seen: 3 hours, 3 minutes
Re: 15 year old Psilocybe stuntzii spore microscopy [Re: BlimeyGrimey]
    #28637110 - 01/27/24 04:15 PM (3 months, 19 days ago)

Quote:

BlimeyGrimey said:
Does the ATP withstand sterilization temps?






I can't really answer this, but in my experience I always filtered it with a 0.22 uM filter instead of autoclave, but that was with very pure water as well. If you have that option, it may be preferred over autoclaving.

Cool micrographs!


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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,799
Loc: Puget Sound
Re: 15 year old Psilocybe stuntzii spore microscopy [Re: Pscientist]
    #28637140 - 01/27/24 04:46 PM (3 months, 19 days ago)

Yup, that's what I've been doing with 0.22um wheel filters and grocery store distilled water. Haven't had a single contamination issue yet using that method. Though sterile wheel filters get expensive, so I make 30ml batches at a time into three sterile 10ml glass vials.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

Extras: Filter Print Post Top
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