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OfflineKinichi
Stranger
Male

Registered: 12/13/23
Posts: 9
Loc: Midwest
Last seen: 2 months, 27 days
Concentrating Liquid Myc
    #28607164 - 01/02/24 02:04 PM (4 months, 13 days ago)

I have searched the posts and could not find anything that takes into account the concentration level of mycelium in a liquid cultures. There are many great recipes and techniques here, but there comes a point when any LC will slow or stop expanding in the media its growing. At this point there is a portion of the nutrient mixture that is not going to be consumed. This becomes the separator solution when shaking/mixing the LC.

MY THOUGHTS or QUESTIONS:
1. Some of the media will already be infused into the myco blob that is growing - IS ALL OF THE REMAINING NECESSARY OR BENEFICIAL TO INOCULATION?

2. If you pull out some of the unused media prior to shaking/mixing -
    WILL THE MYC STILL SEPARATE ENOUGH TO PULL THROUGH AN 18 GA SYRINGE?
    WILL THE INCREASED CONCENTRATION SPEED UP COLONIZATION?

My suspicion is that having a greater concentration of myco that can pass through the syringe might accomplish a couple things.

A. The nutrient solution will be used faster and the mycelium will search out new nutrient sources (Bulk Spawn or Cakes or whatever is being used).

B. The decrease in moisture added to the bulk could help reduce opportunistic contam that was waiting for a little more water to start growing. 

C. It might decrease total colonization time for the Bulk or Cake.

I am getting ready to run an experiment that will test this theory in a few ways over a few different Bulk set ups with some LC that i have that has just about stopped expanding. If you have any experience with this theory or any recomendations for possible vectors to address let me know.

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OfflineHelloImBob
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I'm a teapot
Registered: 03/30/08
Posts: 242
Last seen: 3 months, 15 days
Re: Concentrating Liquid Myc [Re: Kinichi]
    #28620756 - 01/14/24 10:03 AM (4 months, 1 day ago)

If your trying to experiment why wouldn't you start with the easiest possible method?

Get yourself some larger gauge needles such as 14ga for LC and before you shake your jar remove as much of the liquid as you can.

It may help in the future to get replies if your posts in advanced mycology sound like it's worth replying to. This isn't anything goes general cultivation and the people who would reply expect you to have a certain level of intelligence for it to be worth their time.

Sorry not trying to be rude or mean that's just how it is.


--------------------
Quote from Stipe-n-Cap

"You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."

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OfflineKinichi
Stranger
Male

Registered: 12/13/23
Posts: 9
Loc: Midwest
Last seen: 2 months, 27 days
Re: Concentrating Liquid Myc [Re: HelloImBob]
    #28622599 - 01/15/24 07:35 PM (4 months, 39 minutes ago)

No worries mate.

The forums used to be a place where you could bring up questions or theories about different approaches and discuss their possibilities. Definitely not that way anymore.

"If your trying to experiment why wouldn't you start with the easiest possible method?" 

Which method would that be?



All good

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OfflineHelloImBob
Old Guy
I'm a teapot
Registered: 03/30/08
Posts: 242
Last seen: 3 months, 15 days
Re: Concentrating Liquid Myc [Re: Kinichi]
    #28624652 - 01/17/24 02:21 PM (3 months, 29 days ago)

Get yourself some larger gauge needles such as 14ga for LC and before you shake your jar remove as much of the liquid as you can. (Possible contam issues if not done correctly)

Or let the mycelium settle to the bottom of your sryinge and push out the extra liquid before using and repeat

Personally I don't see much benefit to concentrating since you can colonize way faster than you can fruit already with LC unless I'm missing something

4-7 days for a quart seems fast enough


--------------------
Quote from Stipe-n-Cap

"You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."

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OfflineBlufuut180
professional moron

Registered: 11/14/23
Posts: 30
Last seen: 12 days, 12 hours
Re: Concentrating Liquid Myc [Re: HelloImBob]
    #28656707 - 02/12/24 12:13 PM (3 months, 3 days ago)

I used to use a higher nutrient liquid culture with a pinch of agar to make it a very soupy consistency. It grew insane amounts of mycelium for a liquid medium and was so aggressive it would creep up the jars if left unstirred for a long time. This however caused all of them to become contaminated after about a month. I do think that the higher concentration is good for colonization time, specifically that initial jump off. However due to the loss rate I have since gone back to a 4% corn syrup solution and a pinch of peptone. Have had much better results overall.

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