Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Offlinedrbobgoatse
Stranger

Registered: 03/04/21
Posts: 36
Last seen: 2 years, 6 months
Agar growth ID
    #27285593 - 04/28/21 07:36 PM (2 years, 10 months ago)

Long time reader, first time poster.

I am having a go at growing mycelium from spores on agar, however I am not certain if what is growing here is very fluffy mycelium or mold.

The growth did all radiate out from where the spores were clumsily scraped onto the plates, however it looks a lot fluffier than most of the photos I have seen posted on the forum.

If this is indeed mycelium, should I select for a sector of rhizomorphic growth or just the fastest growing fluffy area?

Based on recent reading I believe my selection should have been made a few days ago.


Extras: Filter Print Post Top
InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Agar growth ID [Re: drbobgoatse] * 1
    #27285597 - 04/28/21 07:38 PM (2 years, 10 months ago)

Spore plates always looked fucked to me.
I'd jst start taking transfers. Grow em, clean em, drop em in grain.

Extras: Filter Print Post Top
Offlinetiptrippy
The Mechanic
Male


Registered: 09/09/20
Posts: 1,131
Loc: United States Flag
Last seen: 1 year, 8 months
Re: Agar growth ID [Re: Feasoghorm]
    #27285661 - 04/28/21 08:13 PM (2 years, 10 months ago)

:whathesaid:

Extras: Filter Print Post Top
InvisibleLenz
Misunderestimated
I'm a teapot User Gallery


Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: Agar growth ID [Re: tiptrippy]
    #27285688 - 04/28/21 08:39 PM (2 years, 10 months ago)

Yeah I'd try and take a decent amount of transfers from a few different spots just to be safe, I've accidentally transferred mold before from a germ plate and I was glad I decided to take a couple other transfers. Usually it's fine but sometimes people don't make the cleanest prints lol.


--------------------
:chems:

Extras: Filter Print Post Top
Offlinedrbobgoatse
Stranger

Registered: 03/04/21
Posts: 36
Last seen: 2 years, 6 months
Re: Agar growth ID [Re: Lenz]
    #27285733 - 04/28/21 09:21 PM (2 years, 10 months ago)

Thank you, will try 3 transfers from each plate and see how it goes.

Extras: Filter Print Post Top
OfflineNotarealguy
Stranger danger
 User Gallery

Registered: 02/10/21
Posts: 789
Last seen: 2 years, 2 months
Re: Agar growth ID [Re: drbobgoatse]
    #27285736 - 04/28/21 09:22 PM (2 years, 10 months ago)

I like the looks of the nickle sized dot on the bottom of right picture.  6 o'clock

Also the nickle 10 o'clock on the right picture.

I'd go for the rhizo growth.    Spores to agar tend to be fluffy until it grows out, or you make a transfer.

Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Agar growth ID [Re: Notarealguy]
    #27285742 - 04/28/21 09:25 PM (2 years, 10 months ago)

I'd take two transfers from the 12 position of the first plate.

Extras: Filter Print Post Top
OfflineNotarealguy
Stranger danger
 User Gallery

Registered: 02/10/21
Posts: 789
Last seen: 2 years, 2 months
Re: Agar growth ID [Re: drbobgoatse] * 1
    #27285751 - 04/28/21 09:37 PM (2 years, 10 months ago)

Verum has way more agar experience than i do, so I'd listen to his recommendation.

Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Agar growth ID [Re: Notarealguy] * 1
    #27285764 - 04/28/21 09:44 PM (2 years, 10 months ago)

Much obliged but without a better pic, it's just an edumacated guess.

Extras: Filter Print Post Top
InvisibleAtomsplit
 User Gallery


Registered: 01/16/21
Posts: 1,511
Loc: SAB
Re: Agar growth ID [Re: verum subsequentis]
    #27285765 - 04/28/21 09:45 PM (2 years, 10 months ago)

Quote:

verum subsequentis said:
I'd take two transfers from the 12 position of the first plate.




Wow took me a minute to figure out why. Is it because 12 has long fine hair like strands reaching out? :bow2:


--------------------
.

Edited by Atomsplit (04/28/21 09:46 PM)

Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Agar growth ID [Re: verum subsequentis] * 1
    #27285766 - 04/28/21 09:46 PM (2 years, 10 months ago)

If you post a nice clear backlit pick of each looking from the bottom side of the dish, I'll tell you what I really think.

Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Agar growth ID [Re: Atomsplit] * 1
    #27285769 - 04/28/21 09:48 PM (2 years, 10 months ago)

Quote:

Atomsplit said:
Quote:

verum subsequentis said:
I'd take two transfers from the 12 position of the first plate.




Wow took me a minute to figure out why. Is it because 12 has long fine hair like strands reaching out? :bow2:





Yes. Which translate to, less genetics (and possibly bacteria) piled up on each other. If I'm correct, Transferring from there leads to cleaner looking plates that are much easier to ID, much quicker.

Again, Would need better pics to confirm.

Extras: Filter Print Post Top
InvisibleAtomsplit
 User Gallery


Registered: 01/16/21
Posts: 1,511
Loc: SAB
Re: Agar growth ID [Re: verum subsequentis]
    #27285773 - 04/28/21 09:56 PM (2 years, 10 months ago)

Quote:

verum subsequentis said:
Quote:

Atomsplit said:
Quote:

verum subsequentis said:
I'd take two transfers from the 12 position of the first plate.




Wow took me a minute to figure out why. Is it because 12 has long fine hair like strands reaching out? :bow2:





Yes. Which translate to, less genetics (and possibly bacteria) piled up on each other. If I'm correct, Transferring from there leads to cleaner looking plates that are much easier to ID, much quicker.

Again, Would need better pics to confirm.




Thanks A LOT for your time and help explaining this. :mushroom2:


--------------------
.

Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Agar growth ID [Re: Atomsplit]
    #27285780 - 04/28/21 10:02 PM (2 years, 10 months ago)

:goodday:
My pleasure.

Extras: Filter Print Post Top
Offlinedrbobgoatse
Stranger

Registered: 03/04/21
Posts: 36
Last seen: 2 years, 6 months
Re: Agar growth ID [Re: verum subsequentis] * 1
    #27285877 - 04/28/21 11:46 PM (2 years, 10 months ago)

Quote:

verum subsequentis said:
If you post a nice clear backlit pick of each looking from the bottom side of the dish, I'll tell you what I really think.




Not going to turn down such a fine offer.

Is my ghetto light box bright enough on these?








Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Agar growth ID [Re: drbobgoatse]
    #27286377 - 04/29/21 09:56 AM (2 years, 10 months ago)

It's a little dull but still good. I like nice bright lights right behind her.




I still like the portion I said before but I can also now see that I'd take from the 8-9 portion of the last pic. You see were it falls away from the wall of the petri and leaves you with that nice little "leading edge"? I'd snatch one or two from there as well.

You can obviously grab a handful and then grab a handful from each of them on the next and so on and so on... But I'd just caution you not to get carried away. Taking a few at first to up your chances of success is fine but there are more than enough genetics available in each tiny transfer.

Extras: Filter Print Post Top
Offlinedrbobgoatse
Stranger

Registered: 03/04/21
Posts: 36
Last seen: 2 years, 6 months
Re: Agar growth ID [Re: verum subsequentis]
    #27286743 - 04/29/21 03:15 PM (2 years, 10 months ago)

Thank you very much.

Is the reasoning at this stage essentially that those sectors have not yet met up with others so will hold fewer genetics, or is there also a growth rate factor in play?

All the reading in the world about selecting for transfers did not really prepare me for what I saw directly from spores, nothing quite like first hand experience.

Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Agar growth ID [Re: drbobgoatse]
    #27286828 - 04/29/21 04:24 PM (2 years, 10 months ago)

Nothing like first hand experience, indeed.

Spores to grain is a mess. Sometimes because there are little nasty shits hiding in the mix, sometimes because there are genes on genes on genes on... and sometimes it's both. The reason I would choose those areas is just because they are growing from germ points outward into open space. This makes it easier to see what's up and means that you're are only grabbing one batch of thousands and thousands of genetics instead of two different batches. The sooner you get to fewer genetics available the sooner your plate will be able to be ID as clean.

This is why streaking plates is great. Spread that shit around and end up able to see everything and only grab what ya want.

Extras: Filter Print Post Top
Offlinedrbobgoatse
Stranger

Registered: 03/04/21
Posts: 36
Last seen: 2 years, 6 months
Re: Agar growth ID [Re: verum subsequentis] * 1
    #27296430 - 05/06/21 12:54 AM (2 years, 10 months ago)

Thanks to the guidance of forum members, two of my transfers are looking pretty good I think.

I should have kept track of which new plates came from where on the old ones, another lesson learned.

Do either of these now look appropriate to head to grain, or would another transfer be wise? I cannot see any obvious contamination:

Plate 1:


Plate 2 (please excuse clumsy first time agar pour):

Extras: Filter Print Post Top
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 1,852
Re: Agar growth ID [Re: drbobgoatse]
    #27296608 - 05/06/21 06:43 AM (2 years, 10 months ago)

Those look great, well done!

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* how long for agar growth? Anonymous 2,239 2 12/07/02 09:53 AM
by rhizo
* Agar Questions cantara 5,236 6 08/09/01 06:22 AM
by ar393
* Agar Questions Rohypnol 1,270 5 10/28/03 03:37 PM
by Mycomancer
* question about using agar to test my brew? crazytab 780 9 01/05/04 09:13 PM
by Yarry
* AGAR ENVY! (Anything and All things agar!)
( 1 2 3 4 ... 1280 1281 )
TormatoMFacebookDiscordReddit 552,671 25,612 03/26/24 08:12 PM
by trippytimes
* First Time Agar//Mold or Mycelium? Steez1747 594 11 05/07/21 08:19 PM
by Steez1747
* very slow, clear growth on agar *DELETED*
( 1 2 all )
psh 1,889 22 05/21/21 02:47 PM
by Mycelium Juice
* Benefits of Agar? celsius 9,457 10 12/01/19 12:36 AM
by wildernessjunkie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
620 topic views. 12 members, 74 guests and 113 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.005 seconds on 14 queries.