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drbobgoatse
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Agar growth ID
#27285593 - 04/28/21 07:36 PM (2 years, 10 months ago) |
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Long time reader, first time poster.
I am having a go at growing mycelium from spores on agar, however I am not certain if what is growing here is very fluffy mycelium or mold.
The growth did all radiate out from where the spores were clumsily scraped onto the plates, however it looks a lot fluffier than most of the photos I have seen posted on the forum.
If this is indeed mycelium, should I select for a sector of rhizomorphic growth or just the fastest growing fluffy area?
Based on recent reading I believe my selection should have been made a few days ago.
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Feasoghorm
Registered: 10/24/18
Posts: 4,384
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Spore plates always looked fucked to me. I'd jst start taking transfers. Grow em, clean em, drop em in grain.
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tiptrippy
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Lenz
Misunderestimated
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Yeah I'd try and take a decent amount of transfers from a few different spots just to be safe, I've accidentally transferred mold before from a germ plate and I was glad I decided to take a couple other transfers. Usually it's fine but sometimes people don't make the cleanest prints lol.
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drbobgoatse
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Re: Agar growth ID [Re: Lenz]
#27285733 - 04/28/21 09:21 PM (2 years, 10 months ago) |
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Thank you, will try 3 transfers from each plate and see how it goes.
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Notarealguy
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I like the looks of the nickle sized dot on the bottom of right picture. 6 o'clock
Also the nickle 10 o'clock on the right picture.
I'd go for the rhizo growth. Spores to agar tend to be fluffy until it grows out, or you make a transfer.
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verum subsequentis
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I'd take two transfers from the 12 position of the first plate.
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Notarealguy
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Verum has way more agar experience than i do, so I'd listen to his recommendation.
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verum subsequentis
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Much obliged but without a better pic, it's just an edumacated guess.
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Atomsplit
Registered: 01/16/21
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Quote:
verum subsequentis said: I'd take two transfers from the 12 position of the first plate.
Wow took me a minute to figure out why. Is it because 12 has long fine hair like strands reaching out?
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Edited by Atomsplit (04/28/21 09:46 PM)
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verum subsequentis
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If you post a nice clear backlit pick of each looking from the bottom side of the dish, I'll tell you what I really think.
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verum subsequentis
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Re: Agar growth ID [Re: Atomsplit] 1
#27285769 - 04/28/21 09:48 PM (2 years, 10 months ago) |
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Quote:
Atomsplit said:
Quote:
verum subsequentis said: I'd take two transfers from the 12 position of the first plate.
Wow took me a minute to figure out why. Is it because 12 has long fine hair like strands reaching out?
Yes. Which translate to, less genetics (and possibly bacteria) piled up on each other. If I'm correct, Transferring from there leads to cleaner looking plates that are much easier to ID, much quicker.
Again, Would need better pics to confirm.
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Atomsplit
Registered: 01/16/21
Posts: 1,511
Loc: SAB
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Quote:
verum subsequentis said:
Quote:
Atomsplit said:
Quote:
verum subsequentis said: I'd take two transfers from the 12 position of the first plate.
Wow took me a minute to figure out why. Is it because 12 has long fine hair like strands reaching out?
Yes. Which translate to, less genetics (and possibly bacteria) piled up on each other. If I'm correct, Transferring from there leads to cleaner looking plates that are much easier to ID, much quicker.
Again, Would need better pics to confirm.
Thanks A LOT for your time and help explaining this.
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verum subsequentis
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My pleasure.
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drbobgoatse
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Quote:
verum subsequentis said: If you post a nice clear backlit pick of each looking from the bottom side of the dish, I'll tell you what I really think.
Not going to turn down such a fine offer.
Is my ghetto light box bright enough on these?
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verum subsequentis
seeker of truth
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It's a little dull but still good. I like nice bright lights right behind her.
I still like the portion I said before but I can also now see that I'd take from the 8-9 portion of the last pic. You see were it falls away from the wall of the petri and leaves you with that nice little "leading edge"? I'd snatch one or two from there as well.
You can obviously grab a handful and then grab a handful from each of them on the next and so on and so on... But I'd just caution you not to get carried away. Taking a few at first to up your chances of success is fine but there are more than enough genetics available in each tiny transfer.
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drbobgoatse
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Thank you very much.
Is the reasoning at this stage essentially that those sectors have not yet met up with others so will hold fewer genetics, or is there also a growth rate factor in play?
All the reading in the world about selecting for transfers did not really prepare me for what I saw directly from spores, nothing quite like first hand experience.
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verum subsequentis
seeker of truth
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Nothing like first hand experience, indeed.
Spores to grain is a mess. Sometimes because there are little nasty shits hiding in the mix, sometimes because there are genes on genes on genes on... and sometimes it's both. The reason I would choose those areas is just because they are growing from germ points outward into open space. This makes it easier to see what's up and means that you're are only grabbing one batch of thousands and thousands of genetics instead of two different batches. The sooner you get to fewer genetics available the sooner your plate will be able to be ID as clean.
This is why streaking plates is great. Spread that shit around and end up able to see everything and only grab what ya want.
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drbobgoatse
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Thanks to the guidance of forum members, two of my transfers are looking pretty good I think.
I should have kept track of which new plates came from where on the old ones, another lesson learned.
Do either of these now look appropriate to head to grain, or would another transfer be wise? I cannot see any obvious contamination:
Plate 1:
Plate 2 (please excuse clumsy first time agar pour):
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LadysKnight
Hello Ladies
Registered: 10/09/15
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Those look great, well done!
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