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Offlineotsilla
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Is it mold or myc and where to transfer from?
    #26509363 - 02/29/20 07:59 AM (4 years, 29 days ago)

So, it's my first time working on agar, did a MS print to agar, but the growth is either really fluffy myc or white mold, can't really tell. So my question is, if it's mold, then is there any way to get clean myc out of this or should I toss it? Or is there somewhere possible to do the transfer.

So I added pictures from front and back, also one with blue and green lines, where the myc seems to be the strongest in my opinion or where it would be possible to transfer.




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InvisibleLadysKnight
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Re: Is it mold or myc and where to transfer from? [Re: otsilla] * 2
    #26509368 - 02/29/20 08:03 AM (4 years, 29 days ago)

Looks fine. I like the area with green lines.

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: LadysKnight]
    #26530968 - 03/12/20 12:39 PM (4 years, 17 days ago)

So I did a transfer from both just in case, one seems to be faster than the other, so should I toss the slower one?

Also have a few other questions.

Is that little dot up right a bacterial colony in B dish? Only way is to transfer once again right, if so, then where would be the best place? From 1-3 o clock?

Also, A looks slower but otherwise healthy, should I maybe grow that out and transfer to grain and grow it out or will the growth stay slow and theres no bother?





Edited by otsilla (03/12/20 12:40 PM)

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26537777 - 03/16/20 02:16 AM (4 years, 14 days ago)

bump

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26539848 - 03/17/20 02:18 AM (4 years, 13 days ago)

up

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InvisibleLadysKnight
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26540109 - 03/17/20 07:09 AM (4 years, 12 days ago)

That dot could be bacteria, yes.
Go with your plan on B. I would keep transferring the best until it looks more geometrically balanced.
A is likely slower due to the ring of contamination around it, seen best in 3rd pic, 2:00. B has it too at 5:00

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: LadysKnight]
    #26540236 - 03/17/20 08:20 AM (4 years, 12 days ago)

Dont really care that much about geometry at this point, I wanna get my grains started and get some clones from grow and go from there.

But damn, you want to say that both of them are contaminated all around? What is that grey little stuff all around? It always seems to be a small line around the growing edge, so I thought it was mycelium or maybe lost moisture from agar, haven't seen any other pictures like that also when I searched for different agar contams. You can see it really well from behind pictures.

So should I toss those or how could I get them clean if its all around?

Edited by otsilla (03/17/20 08:23 AM)

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InvisibleLadysKnight
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26540255 - 03/17/20 08:30 AM (4 years, 12 days ago)

I'd attempt to clean it up with transfers, but also start new spores in case you can't.

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Offlinerumfor69
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Re: Is it mold or myc and where to transfer from? [Re: LadysKnight]
    #26540272 - 03/17/20 08:37 AM (4 years, 12 days ago)

They look pretty good just keep transferring and trying to
isolate that ropy rhizo mycelium.

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: rumfor69]
    #26540817 - 03/17/20 01:36 PM (4 years, 12 days ago)

Alright, so those two last posts are really confusing :laugh: So I took a couple of more shots in better lightning. As you can see that kind of ring is around every plate. How would I even clean that up if it's everywhere? Couldn't I take that vertical growth from A's plate, or would that be contaminated also? Or it isn't contamination afterall.

Up close it really looks like nothing, just a coarser surface. Suggestions? :confused:








Edited by otsilla (03/17/20 01:42 PM)

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Offlinerumfor69
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26540961 - 03/17/20 02:46 PM (4 years, 12 days ago)

I would let it keep going a little longer to see if it shoots out

further anywhere to get away from the ring and stuff.

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InvisibleLadysKnight
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26541135 - 03/17/20 04:31 PM (4 years, 12 days ago)

Those pics are better, but still can't clearly see what's up. But yes, they're contaminated. Considering it seems to have some form, I think it's mold. I don't think you'll be able to transfer away from it. I'd go back to spores on agar. If you have a syringe, make a few small brf cakes and noc those up at the same time, you should eventually get a fruit to clone, which will give you another simultaneous path to a clean culture.

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: rumfor69]
    #26542279 - 03/18/20 08:47 AM (4 years, 11 days ago)

Alright, so I did a bit more digging. And thats what I found.

https://www.shroomery.org/forums/showflat.php/Number/26067744#26067744
https://www.shroomery.org/forums/showflat.php/Number/20691796

Seems to be a normal thing that happens sometimes. I think I'll let the A plate grow out and trasnfer to grains, see what happens. I'll do one more transfer to B tho as it probably has a bacterial colony on top right, maybe transfer not from the very leading edge just to see? But as it is barely visible with a naked eye, and just like a coarser surface, nothing more I keep leaning towards a microscopic mycelium.

Also the agar is bought sterilized in a bottle, its made from potato starch, dextrose and agar.

Edited by otsilla (03/18/20 08:48 AM)

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InvisibleLadysKnight
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26542293 - 03/18/20 08:59 AM (4 years, 11 days ago)

To know for sure, take transfers of just the halo, see what grows on the plate.

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: LadysKnight]
    #26542304 - 03/18/20 09:08 AM (4 years, 11 days ago)

Alright, I might try that, if it's really a microscopic myc it should grow out as healthy myc?

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InvisibleLadysKnight
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26542311 - 03/18/20 09:12 AM (4 years, 11 days ago)

Yep. But my money says it will make a mostly clearish mold culture.

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: LadysKnight]
    #26542324 - 03/18/20 09:22 AM (4 years, 11 days ago)

Shouldn't mold be quicker and more unexpected than myc tho? Or grow bigger, change shape etc. I mean it's always this translucent ring around the leading edge, nothing more.

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InvisibleLadysKnight
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Re: Is it mold or myc and where to transfer from? [Re: otsilla]
    #26542329 - 03/18/20 09:28 AM (4 years, 11 days ago)

Some will say this is always the case. It is not. Some mold will grow just ahead of mush myc, in a clear halo. I'm working with several cultures like this right now.

That being said, your pics still don't show the halo very well, and there are many types. If you hold a plate with the light hitting the right angle, do they look like this:

https://www.shroomery.org/forums/showflat.php/Number/26506426#26506426

Edited by LadysKnight (03/18/20 09:47 AM)

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Offlineotsilla
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Re: Is it mold or myc and where to transfer from? [Re: LadysKnight]
    #26574802 - 04/03/20 08:56 AM (3 years, 11 months ago)

So, decided to wait a bit to see what will happen, meanwhile I started another two plates from MS, same problem, the growth is really translucent. But now its not so vague, I can see strands. Started them 26th of march and just yesterday I saw first white puffs. My concern is, is that myc or is it mold, and the translucent part is clean myc? It's strange because the white part doesn't start from directly where the spores were. Should I just transfer the transparent part now and see what happens? On my first plates the transparent parts were just a bit discolored, but now upon closer inspection the new ones have like strands, i can define it. Really confused at this point what would be my best bet.

Even though it's hard to catch it on camera exactly I'll add some pics. Also added my very first master, which still doesn't seem to have gone bad, another one is showing up mold on top left, and the other one I opened up to smell it, smelled like mushrooms, but after a day or so started smelling like mold, maybe it was because of the opening, i dont know.










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Offlinepoisoned
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Re: Is it mold or myc and where to transfer from? [Re: otsilla] * 1
    #26574908 - 04/03/20 09:58 AM (3 years, 11 months ago)

I often get that transparent leading edge. It turns out to be myc 99% of the time. Check against the light and if it has tiny filaments, you're probably good. Usually, it gets better if you lower your nutes and after a few transfers. What's your agar recipe?


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