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OfflineUnagiShark
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Successful tissue clone on agar of Agaricus Bisporus (minis), I believe * 1
    #26420279 - 01/07/20 01:47 AM (4 years, 1 month ago)

I should be looking for a small circular Mycellium growth somewhat separated from the tissue used for the cloning, correct? I hardly think its an accidental cyan or allenii mycellium, as I do use proper sanitary practice (working over open stove on lowest heat possible and with fan on, gloves, mask, lab smock, high concentration isopropyl, pc as autoclave, etc.).

If its what I'm going for then I'm pretty satisfied. There's no substitute for the knowledge that would come from actually studying the science in full. Not a lot of information on the internet feels reliable when using a google search with not very much knowledge of the search subject at all, to begin with.


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OfflinecronicrFacebook
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Re: Successful tissue clone on agar of Agaricus Bisporus (minis), I believe (moved) [Re: UnagiShark]
    #26420617 - 01/07/20 09:15 AM (4 years, 1 month ago)

This thread was moved from Mushroom Cultivation.

Reason:
Better here


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Invisiblemurderlabz
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Registered: 05/18/19
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Re: Successful tissue clone on agar of Agaricus Bisporus (minis), I believe (moved) [Re: cronicr]
    #26422059 - 01/07/20 09:51 PM (4 years, 1 month ago)

Here is some examples to compare to.

http://www.journalijar.com/uploads/694_IJAR-4936.pdf

This strain kicked my ass, I never go it going. It's still on my bucket list.

I hope all go well.


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OfflineUnagiShark
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Registered: 04/28/18
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Loc: North San Francisco Bay Area, ... Flag
Last seen: 1 month, 23 days
Re: Successful tissue clone on agar of Agaricus Bisporus (minis), I believe (moved) [Re: murderlabz]
    #26427508 - 01/11/20 03:01 AM (4 years, 1 month ago)

Awesome! Thanks a bunch that sure saves time haha. My tissue clone didnt take. Very small mycellial growth and it all got contaminated before I could save it at all.


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