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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 481
Last seen: 11 days, 9 hours
Making home made antibacterial agar any suggestions?
    #25166186 - 04/26/18 05:52 AM (5 years, 10 months ago)

So what I've come up with so far in my research phase of creating some antibacterial agar I found a research paper on the affects of temperature on the most common antibiotics and it showed that penicillin V is the most resistant to high heat followed by penicillin  B and the worst heat tolerant antibiotic was amoxicillin. (See screenshot)



So I ordered some penicillin V in fish medical form these are 250 mg of penicillin V so how much would I add to my agar mixture if I was to make the standard 500ml MAE agar? Can you over do it with antibiotics?



I believe i have a very nasty bacterial infection going on my agar, my wbs jars, my RGS jars, and my mono tubs all have a slight smell idk how to describe it I wanna say a fermented smell but not quite idk but I've been doing agar transfers about 8 times now and no matter how small a chunk from the leading edge it always get this odd smell to it so I think I got some bacteria that just grows along with the myc.

So I'm hoping to make some antibacterial agar and do a few hot pours over a dish that doesnt look contaminated but doesnt smell like fresh clean myc.

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InvisiblebodhisattaMDiscordReddit
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Re: Making home made antibacterial agar any suggestions? [Re: more4u2c]
    #25166380 - 04/26/18 08:36 AM (5 years, 10 months ago)

Most fermentation is of beer and wine so when people think fermentation smell its usually yeasty. Which antibiotics don't kill.

Post pics of the dishes. Antibiotics are almost always unnecessary

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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 481
Last seen: 11 days, 9 hours
Re: Making home made antibacterial agar any suggestions? [Re: bodhisatta]
    #25166883 - 04/26/18 01:53 PM (5 years, 10 months ago)

Sorry for sorta bad pics best I can do atm, it's all white all the same white viewing it from up top its thin and fuzzy when I shine light thru you can see the riznos(sp?) This is a PE variety this plate is 4 transfers from MS


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OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 10 months
Re: Making home made antibacterial agar any suggestions? [Re: more4u2c]
    #25166949 - 04/26/18 02:18 PM (5 years, 10 months ago)

What bod said, antibiotics in agar are mainly useless because big contam problems come from other fungi from the penicillium and aspergillus kind. Nasty mofuckers who grow fast, mix with your myc and sporulate all over the place.

Bacteria on the other side if you're lucky and catch it on time will make isolated colonies. Then you can transfer healthy myc to another dish without having to through out and no trouble opening it.


--------------------
:awecid:

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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 481
Last seen: 11 days, 9 hours
Re: Making home made antibacterial agar any suggestions? [Re: Madoxx]
    #25168337 - 04/27/18 04:00 AM (5 years, 10 months ago)

So what should I do with these agar dishes would a hot pour over an existing dish help or am I screwed and need new syringes

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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 481
Last seen: 11 days, 9 hours
Re: Making home made antibacterial agar any suggestions? [Re: more4u2c]
    #25168399 - 04/27/18 05:16 AM (5 years, 10 months ago)

so as I was taking samples a drip of antibac soap and/or bleach drips from the top of glove box right into the dish so I closed it off and was gonna throw it away well today I check it and it's got a lot of bluing but the suspected contam the fuzzier stuff that always grows out first before the thicker rinzo stuff catches up is still white. So could my drop of soap/bleach be showing me the actual myc (turning blue dying due to the bleach drop) and the contam that's still white and not effected as much by the bleach?

Would a hot pour over the newly made dishes (pictured below) once they grow a little bit more assist the myc and slow down the fuzzy white contam?



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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 481
Last seen: 11 days, 9 hours
Re: Making home made antibacterial agar any suggestions? [Re: more4u2c]
    #25168400 - 04/27/18 05:16 AM (5 years, 10 months ago)


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OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 2 years, 10 months
Re: Making home made antibacterial agar any suggestions? [Re: more4u2c]
    #25175534 - 04/30/18 12:53 PM (5 years, 10 months ago)

Hot pour will work for bacteria (as the dont spread well through semisolid media) but i the molds i mentioned above will destroy it. I can't quite make out what you're worried about on your plates man.

On the pictures two posts up, what is missing i guess is from doing the transfer? and if what you're worrying about it the second front of growth in front of the whiter myc, around the top it looks like filaments so its either myc or another fungi, no reason really to do a hot pour then. Or try getting some good pics of the exact area of the problem so i can help


--------------------
:awecid:

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