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 bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 10 months
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 Inoculating through polyfill? 
#25035056 - 03/03/18 01:19 AM (6 years, 1 month ago) |
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Would you all recommended inoculating through my polyfil stuffed hole in the top of the jar or opening the lid and inoculating in my SAB? Both would be done in my SAB. Just seeing which one is a better idea.
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 MrRabbit-HMR
Professional Duck Washer
Registered: 08/20/17
Posts: 262
Loc: UK 
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Re: Inoculating through polyfill? [Re: bandit864]
#25035105 - 03/03/18 02:55 AM (6 years, 1 month ago) |
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If you can ensure your polyfil doesn't get wet, then going through the polyfil is probably fine, though not ideal. You should look into using an injection port or getting some high temperature silicone and making your own injection port and inoculating through that.
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LemurLemur
Pray for Boog
Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof 
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Re: Inoculating through polyfill? [Re: bandit864]
#25035140 - 03/03/18 03:41 AM (6 years, 1 month ago) |
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Make a SHIP, wipe with bleach, flame needle, inoc, cover with tape of a tiny dab of silicone cooties get in even thfough the tiniest hair line cracks in glass so i dont trust an open nip.
I make my SHIPs 1 on top 1 on the bottom for added durability.
Dont stick the whole needle in just enough for you to inject the solution/ culture.
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  (when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 10 months
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Re: Inoculating through polyfill? [Re: LemurLemur]
#25036078 - 03/03/18 01:47 PM (6 years, 1 month ago) |
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Okay I will make a SHIP on my next jars. The 4 jars I have now with just polyfil should I just open them in my SAB tonight and inoculate them?
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Mateja
Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Inoculating through polyfill? [Re: bandit864]
#25036083 - 03/03/18 01:51 PM (6 years, 1 month ago) |
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That´s how you´re suppose to inoculate with a syringe, open the lid and inject the solution 
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Cakes inside Water Tub
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 10 months
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Re: Inoculating through polyfill? [Re: Mateja]
#25036088 - 03/03/18 01:54 PM (6 years, 1 month ago) |
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Okay cool thank you!
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bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Inoculating through polyfill? [Re: bandit864]
#25036090 - 03/03/18 01:56 PM (6 years, 1 month ago) |
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Poly should be so tight that when you try to inoculate thru it it pushes the polyfil into the jar.
Another issue is that a hot needle melts polyfil.
I don't suggest trying to inoculate thru polyfil tho it can be done
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36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,086
Loc: Diving into Mystical Territori...
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Re: Inoculating through polyfill? [Re: bodhisatta]
#25036103 - 03/03/18 02:04 PM (6 years, 1 month ago) |
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Quote:
bodhisatta said:
Poly should be so tight that when you try to inoculate thru it it pushes the polyfil into the jar.
Another issue is that a hot needle melts polyfil.
I don't suggest trying to inoculate thru polyfil tho it can be done
 exactly.
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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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grow4fun
Registered: 03/02/18
Posts: 171
Last seen: 3 years, 1 month
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Re: Inoculating through polyfill? [Re: 36fuckin5]
#25036124 - 03/03/18 02:15 PM (6 years, 1 month ago) |
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Using poly as a port and GE will work however is more prone to contamination and is a pain in the ass to work with. Buy some RTV and use that as a port or if you want to spend a few bucks buy a rubber SHIP. You can use poly for GE in a 1/4" drilled hole, you may also use tyvek, better yet, a synthetic filter disc.
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FeedYourMind
indiGlo
Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 18 hours
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Re: Inoculating through polyfill? [Re: 36fuckin5]
#25036126 - 03/03/18 02:17 PM (6 years, 1 month ago) |
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I've noc'd up many jars when I first started years ago with a single polyfil stuffed hole and had plenty of success (a couple failures as well). However, the success heavily outweighed any failures I had.
Some of my jars still have the same type of lids, except I mostly go from agar to grain or G2G these days. As Bod stated, if you do go this route, you want to ensure the polyfil is stuffed as tightly as possible.
Though, I've never had issues with the needle pushing the poly through the hole or even burning, as I allow the needle to cool for several seconds before inoculation.
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elasticaltiger
Like Tigers in Coitus
Registered: 06/24/13
Posts: 8,438
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Re: Inoculating through polyfill? [Re: FeedYourMind]
#25036200 - 03/03/18 02:55 PM (6 years, 1 month ago) |
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Just crack the lid and squirt sideways withiut letting anything (hands or syringe) over the opening. Very safe.
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First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it  Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 10 months
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Okay awesome thank y'all for your help. I just knocked up the 4 WBS jars I had with my MS syringes. One GT One PE. How long does it normally take for growth? I'm switching to grain because BRF seems to take a while!
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elasticaltiger
Like Tigers in Coitus
Registered: 06/24/13
Posts: 8,438
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Re: Inoculating through polyfill? [Re: bandit864]
#25036922 - 03/03/18 08:50 PM (6 years, 1 month ago) |
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4 to 7 days to see visible growth. 14 - 25 days for full colonization.
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First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
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Lokimlin
Stranger
Registered: 02/14/18
Posts: 11
Last seen: 3 years, 2 months
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I'm getting 3 days to show growth on my liquid cultures on sterilized milo. you'll definitely want to inject with a liquid culture through a silicone injection port
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36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,086
Loc: Diving into Mystical Territori...
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Re: Inoculating through polyfill? [Re: Lokimlin]
#25038419 - 03/04/18 02:32 PM (6 years, 1 month ago) |
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Quote:
Lokimlin said:
you'll definitely want to inject with a liquid culture through a silicone injection port
That's just the opposite of what I'd do. I'd inoculate grain with agar through a cracked lid. You should be inoculating your LC with agar anyway. Or inoculate grain with a spore syringe through a cracked lid.
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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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sandman420
Saint PP
Registered: 06/17/04
Posts: 5,384
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Re: Inoculating through polyfill? [Re: 36fuckin5]
#25039409 - 03/04/18 10:30 PM (6 years, 1 month ago) |
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I inoculate my grain jars through the poly with no problem. Less than 1% contams. Just keep the foil on 'til you are ready to inoculate.
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LemurLemur
Pray for Boog
Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof
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Re: Inoculating through polyfill? [Re: sandman420]
#25039415 - 03/04/18 10:38 PM (6 years, 1 month ago) |
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An in the pc^ if u go that route. Keep em em in the pc n move direct to the sab regardless
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(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 10 months
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Re: Inoculating through polyfill? [Re: LemurLemur]
#25039418 - 03/04/18 10:40 PM (6 years, 1 month ago) |
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I will post whenever I have an update. No signs of anything yet!
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rabiddoggy
Stranger
Registered: 01/24/18
Posts: 16
Last seen: 9 months, 17 days
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Re: Inoculating through polyfill? [Re: bandit864]
#25040336 - 03/05/18 11:58 AM (6 years, 1 month ago) |
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I have probably inoculated two dozen jars through polyfill without any issues. Good luck 
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 10 months
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Re: Inoculating through polyfill? [Re: rabiddoggy]
#25040422 - 03/05/18 12:59 PM (6 years, 1 month ago) |
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Oh okay next I will inject through the polyfil. The 4 WBS jars I did on the 3rd I opened the lid in my SAB and inoculated. No signs of growth yet though!
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